FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2988804610> ?p ?o ?g. }

• W2988804610 endingPage "9631" @default.
• W2988804610 startingPage "9619" @default.
• W2988804610 abstract "Type I polyketide synthases (PKSs) are large multi-domain proteins converting simple acyl-CoA thioesters such as acetyl-CoA and malonyl-CoA to a large diversity of biotechnologically interesting molecules. Such multi-step reaction cascades are of particular interest for applications in engineered microbial cell factories, as the introduction of a single protein with many enzymatic activities does not require balancing of several individual enzymatic activities. However, functional introduction of type I PKSs into heterologous hosts is very challenging as the large polypeptide chains often do not fold properly. In addition, PKS usually require post-translational activation by dedicated 4′-phosphopantetheinyl transferases (PPTases). Here, we introduce an engineered Corynebacterium glutamicum strain as a novel microbial cell factory for type I PKS-derived products. Suitability of C. glutamicum for polyketide synthesis could be demonstrated by the functional introduction of the 6-methylsalicylic acid synthase ChlB1 from Streptomyces antibioticus. Challenges related to protein folding could be overcome by translation fusion of ChlB1Sa to the C-terminus of the maltose-binding protein MalE from Escherichia coli. Surprisingly, ChlB1Sa was also active in the absence of a heterologous PPTase, which finally led to the discovery that the endogenous PPTase PptACg of C. glutamicum can also activate ChlB1Sa. The best strain, engineered to provide increased levels of acetyl-CoA and malonyl-CoA, accumulated up to 41 mg/L (0.27 mM) 6-methylsalicylic acid within 48 h of cultivation. Further experiments showed that PptACg of C. glutamicum can also activate nonribosomal peptide synthetases (NRPSs), rendering C. glutamicum a promising microbial cell factory for the production of several fine chemicals and medicinal drugs." @default.
• W2988804610 created "2019-11-22" @default.
• W2988804610 creator A5019423861 @default.
• W2988804610 creator A5026546428 @default.
• W2988804610 creator A5035131482 @default.
• W2988804610 creator A5073767583 @default.
• W2988804610 creator A5085419424 @default.
• W2988804610 date "2019-11-04" @default.
• W2988804610 modified "2023-09-29" @default.
• W2988804610 title "Microbial synthesis of the type I polyketide 6-methylsalicylate with Corynebacterium glutamicum" @default.
• W2988804610 cites W1527054665 @default.
• W2988804610 cites W1592573739 @default.
• W2988804610 cites W1677600872 @default.
• W2988804610 cites W1842912074 @default.
• W2988804610 cites W1943327251 @default.
• W2988804610 cites W1951619777 @default.
• W2988804610 cites W1966376668 @default.
• W2988804610 cites W1969915196 @default.
• W2988804610 cites W1969953411 @default.
• W2988804610 cites W1972226822 @default.
• W2988804610 cites W1972740600 @default.
• W2988804610 cites W1978047260 @default.
• W2988804610 cites W1981252549 @default.
• W2988804610 cites W1988948560 @default.
• W2988804610 cites W1994418197 @default.
• W2988804610 cites W2000176823 @default.
• W2988804610 cites W2004064903 @default.
• W2988804610 cites W2009471270 @default.
• W2988804610 cites W2010200303 @default.
• W2988804610 cites W2011631283 @default.
• W2988804610 cites W2013426695 @default.
• W2988804610 cites W2016667166 @default.
• W2988804610 cites W2023229596 @default.
• W2988804610 cites W2023950913 @default.
• W2988804610 cites W2036080658 @default.
• W2988804610 cites W2040686371 @default.
• W2988804610 cites W2044697909 @default.
• W2988804610 cites W2045785445 @default.
• W2988804610 cites W2047172279 @default.
• W2988804610 cites W2047431936 @default.
• W2988804610 cites W2064099757 @default.
• W2988804610 cites W2067943237 @default.
• W2988804610 cites W2072082034 @default.
• W2988804610 cites W2077361309 @default.
• W2988804610 cites W2077736362 @default.
• W2988804610 cites W2078560823 @default.
• W2988804610 cites W2082529739 @default.
• W2988804610 cites W2085943554 @default.
• W2988804610 cites W2101111788 @default.
• W2988804610 cites W2107713423 @default.
• W2988804610 cites W2115955917 @default.
• W2988804610 cites W2116137883 @default.
• W2988804610 cites W2122874689 @default.
• W2988804610 cites W2123607713 @default.
• W2988804610 cites W2126770666 @default.
• W2988804610 cites W2127028584 @default.
• W2988804610 cites W2130127223 @default.
• W2988804610 cites W2144885296 @default.
• W2988804610 cites W2148520571 @default.
• W2988804610 cites W2150158773 @default.
• W2988804610 cites W2159489517 @default.
• W2988804610 cites W2164679484 @default.
• W2988804610 cites W2172492490 @default.
• W2988804610 cites W2291242534 @default.
• W2988804610 cites W2301253691 @default.
• W2988804610 cites W2328992150 @default.
• W2988804610 cites W2411717244 @default.
• W2988804610 cites W2538460648 @default.
• W2988804610 cites W2560074434 @default.
• W2988804610 cites W2787940687 @default.
• W2988804610 cites W2803978374 @default.
• W2988804610 cites W2894520557 @default.
• W2988804610 cites W2912306503 @default.
• W2988804610 cites W2938200072 @default.
• W2988804610 cites W565446105 @default.
• W2988804610 cites W70082989 @default.
• W2988804610 doi "https://doi.org/10.1007/s00253-019-10121-9" @default.
• W2988804610 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31686146" @default.
• W2988804610 hasPublicationYear "2019" @default.
• W2988804610 type Work @default.
• W2988804610 sameAs 2988804610 @default.
• W2988804610 citedByCount "14" @default.
• W2988804610 countsByYear W29888046102020 @default.
• W2988804610 countsByYear W29888046102021 @default.
• W2988804610 countsByYear W29888046102022 @default.
• W2988804610 countsByYear W29888046102023 @default.
• W2988804610 crossrefType "journal-article" @default.
• W2988804610 hasAuthorship W2988804610A5019423861 @default.
• W2988804610 hasAuthorship W2988804610A5026546428 @default.
• W2988804610 hasAuthorship W2988804610A5035131482 @default.
• W2988804610 hasAuthorship W2988804610A5073767583 @default.
• W2988804610 hasAuthorship W2988804610A5085419424 @default.
• W2988804610 hasBestOaLocation W29888046101 @default.
• W2988804610 hasConcept C104317684 @default.
• W2988804610 hasConcept C108305142 @default.
• W2988804610 hasConcept C134651460 @default.
• W2988804610 hasConcept C147816474 @default.
• W2988804610 hasConcept C181199279 @default.

• next