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- W2988898885 abstract "Alcohol use disorder is one of the most disabling diseases worldwide. Glial-cell derived neurotrophic factor (Gdnf) shows promising results concerning the inhibition of alcohol consumption in rodent models. We investigated the epigenetic regulation of Gdnf following ethanol consumption and withdrawal in a rat model. 32 Wistar rats underwent 7 weeks of intermittent access to alcohol in a 2-bottle choice (IA2BC). Whole blood, Nucleus Accumbens (NAc) and Ventral Tegmental Area (VTA) were collected immediately after the last 24 h of an alcohol-drinking session (alcohol group, AG) or 24 h after withdrawal (withdrawal group, WG). MRNA levels were measured using real-time quantitative PCR. Bisulfite-conversion of DNA and capillary sequencing was used to determine methylation levels of the core promoter (CP) and the negative regulatory element (NRE). The CP of the AG in the NAc was significantly less methylated compared to controls (p < 0.05). In the NAc, mRNA expression was significantly higher in the WG (p < 0.05). In the WG, mRNA expression levels in the VTA were significantly lower (p < 0.05) and showed significantly less methylation in the NRE in the VTA (p < 0.001) and the NAc (p < 0.01) compared to controls. Changes in the cerebral mRNA expression correspond to alterations in DNA methylation of the Gdnf promoter in a rodent model. Our results hold clinical relevance since differences in Gdnf mRNA expression and DNA methylation could be a target for pharmacological interventions." @default.
- W2988898885 created "2019-11-22" @default.
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- W2988898885 date "2020-02-01" @default.
- W2988898885 modified "2023-09-23" @default.
- W2988898885 title "Alcohol consumption alters Gdnf promoter methylation and expression in rats" @default.
- W2988898885 cites W1427096520 @default.
- W2988898885 cites W1698620799 @default.
- W2988898885 cites W1898776365 @default.
- W2988898885 cites W1963841728 @default.
- W2988898885 cites W1968488639 @default.
- W2988898885 cites W1971208141 @default.
- W2988898885 cites W1972559070 @default.
- W2988898885 cites W1977731479 @default.
- W2988898885 cites W1979236238 @default.
- W2988898885 cites W1980277133 @default.
- W2988898885 cites W1981854032 @default.
- W2988898885 cites W1983444230 @default.
- W2988898885 cites W1988982907 @default.
- W2988898885 cites W1989457196 @default.
- W2988898885 cites W1994452959 @default.
- W2988898885 cites W1997008618 @default.
- W2988898885 cites W2005029096 @default.
- W2988898885 cites W2012246166 @default.
- W2988898885 cites W2017166426 @default.
- W2988898885 cites W2022090925 @default.
- W2988898885 cites W2026255880 @default.
- W2988898885 cites W2029725727 @default.
- W2988898885 cites W2033355894 @default.
- W2988898885 cites W2034210515 @default.
- W2988898885 cites W2036178921 @default.
- W2988898885 cites W2039276157 @default.
- W2988898885 cites W2041139441 @default.
- W2988898885 cites W2041709722 @default.
- W2988898885 cites W2043320027 @default.
- W2988898885 cites W2044806054 @default.
- W2988898885 cites W2049099403 @default.
- W2988898885 cites W2050061925 @default.
- W2988898885 cites W2050831289 @default.
- W2988898885 cites W2051455449 @default.
- W2988898885 cites W2054810445 @default.
- W2988898885 cites W2055319462 @default.
- W2988898885 cites W2055390989 @default.
- W2988898885 cites W2060342626 @default.
- W2988898885 cites W2061505188 @default.
- W2988898885 cites W2064756496 @default.
- W2988898885 cites W2066848874 @default.
- W2988898885 cites W2069210581 @default.
- W2988898885 cites W2074268143 @default.
- W2988898885 cites W2080133581 @default.
- W2988898885 cites W2082995490 @default.
- W2988898885 cites W2084118479 @default.
- W2988898885 cites W2088864744 @default.
- W2988898885 cites W2090148190 @default.
- W2988898885 cites W2092103977 @default.
- W2988898885 cites W2094138192 @default.
- W2988898885 cites W2098104823 @default.
- W2988898885 cites W2102035457 @default.
- W2988898885 cites W2102240317 @default.
- W2988898885 cites W2105696266 @default.
- W2988898885 cites W2108724455 @default.
- W2988898885 cites W2110673629 @default.
- W2988898885 cites W2114457069 @default.
- W2988898885 cites W2118428015 @default.
- W2988898885 cites W2120690833 @default.
- W2988898885 cites W2122026190 @default.
- W2988898885 cites W2129530731 @default.
- W2988898885 cites W2137385309 @default.
- W2988898885 cites W2142746129 @default.
- W2988898885 cites W2152451432 @default.
- W2988898885 cites W2153998371 @default.
- W2988898885 cites W2155971360 @default.
- W2988898885 cites W2161704465 @default.
- W2988898885 cites W2163712399 @default.
- W2988898885 cites W2164326183 @default.
- W2988898885 cites W2165398609 @default.
- W2988898885 cites W2170891278 @default.
- W2988898885 cites W2174200381 @default.
- W2988898885 cites W2189891878 @default.
- W2988898885 cites W2339186961 @default.
- W2988898885 cites W2479791564 @default.
- W2988898885 cites W2551528399 @default.
- W2988898885 cites W2589341684 @default.
- W2988898885 cites W2591643824 @default.
- W2988898885 cites W2617663206 @default.
- W2988898885 cites W2743932369 @default.
- W2988898885 cites W2781700392 @default.
- W2988898885 cites W2889166105 @default.