Matches in SemOpenAlex for { <https://semopenalex.org/work/W2989025252> ?p ?o ?g. }
- W2989025252 abstract "Abstract Unfertilised eggs (oocytes) release chemical biomarkers into the medium surrounding them. This provides an opportunity to monitor cell health and development during assisted reproductive processes if detected in a non‐invasive manner. Here we report the measurement of pH using an optical fibre probe, OFP1, in 5 μL drops of culture medium containing single mouse cumulus oocyte complexes (COCs). This allowed for the detection of statistically significant differences in pH between COCs in culture medium with no additives and those incubated with either a chemical (cobalt chloride) or hormonal treatment (follicle stimulating hormone); both of which serve to induce the release of lactic acid into the medium immediately surrounding the COC. Importantly, OFP1 was shown to be cell‐safe with no inherent cell toxicity or light‐induced phototoxicity indicated by negative DNA damage staining. Pre‐measurement photobleaching of the probe reduced fluorescence signal variability, providing improved measurement precision (0.01‐0.05 pH units) compared to previous studies. This optical technology presents a promising platform for the measurement of pH and the detection of other extracellular biomarkers to assess cell health during assisted reproduction." @default.
- W2989025252 created "2019-11-22" @default.
- W2989025252 creator A5004353622 @default.
- W2989025252 creator A5033537928 @default.
- W2989025252 creator A5039901364 @default.
- W2989025252 creator A5040076923 @default.
- W2989025252 creator A5043932518 @default.
- W2989025252 creator A5062716203 @default.
- W2989025252 creator A5071910490 @default.
- W2989025252 creator A5075273734 @default.
- W2989025252 creator A5085173358 @default.
- W2989025252 date "2019-12-04" @default.
- W2989025252 modified "2023-09-26" @default.
- W2989025252 title "A biophotonic approach to measure pH in small volumes in vitro: Quantifiable differences in metabolic flux around the cumulus‐oocyte‐complex (COC)" @default.
- W2989025252 cites W1486131515 @default.
- W2989025252 cites W1535466351 @default.
- W2989025252 cites W1606010310 @default.
- W2989025252 cites W1975298497 @default.
- W2989025252 cites W1995618372 @default.
- W2989025252 cites W2005958031 @default.
- W2989025252 cites W2009146329 @default.
- W2989025252 cites W2015301454 @default.
- W2989025252 cites W2044959366 @default.
- W2989025252 cites W2054167608 @default.
- W2989025252 cites W2075551125 @default.
- W2989025252 cites W2079157561 @default.
- W2989025252 cites W2087674181 @default.
- W2989025252 cites W2089247592 @default.
- W2989025252 cites W2099210523 @default.
- W2989025252 cites W2106114981 @default.
- W2989025252 cites W2121070680 @default.
- W2989025252 cites W2139500938 @default.
- W2989025252 cites W2144457833 @default.
- W2989025252 cites W2163299851 @default.
- W2989025252 cites W2164690264 @default.
- W2989025252 cites W2169523801 @default.
- W2989025252 cites W2170530908 @default.
- W2989025252 cites W2171599768 @default.
- W2989025252 cites W2205274219 @default.
- W2989025252 cites W2298206083 @default.
- W2989025252 cites W2327407167 @default.
- W2989025252 cites W2343552610 @default.
- W2989025252 cites W2346683277 @default.
- W2989025252 cites W2461066519 @default.
- W2989025252 cites W2489923389 @default.
- W2989025252 cites W2559323362 @default.
- W2989025252 cites W2626797397 @default.
- W2989025252 cites W2750911893 @default.
- W2989025252 cites W2762807699 @default.
- W2989025252 cites W2890317363 @default.
- W2989025252 cites W2906133203 @default.
- W2989025252 cites W2910038554 @default.
- W2989025252 cites W2915287769 @default.
- W2989025252 doi "https://doi.org/10.1002/jbio.201960038" @default.
- W2989025252 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31725948" @default.
- W2989025252 hasPublicationYear "2019" @default.
- W2989025252 type Work @default.
- W2989025252 sameAs 2989025252 @default.
- W2989025252 citedByCount "6" @default.
- W2989025252 countsByYear W29890252522021 @default.
- W2989025252 countsByYear W29890252522022 @default.
- W2989025252 countsByYear W29890252522023 @default.
- W2989025252 crossrefType "journal-article" @default.
- W2989025252 hasAuthorship W2989025252A5004353622 @default.
- W2989025252 hasAuthorship W2989025252A5033537928 @default.
- W2989025252 hasAuthorship W2989025252A5039901364 @default.
- W2989025252 hasAuthorship W2989025252A5040076923 @default.
- W2989025252 hasAuthorship W2989025252A5043932518 @default.
- W2989025252 hasAuthorship W2989025252A5062716203 @default.
- W2989025252 hasAuthorship W2989025252A5071910490 @default.
- W2989025252 hasAuthorship W2989025252A5075273734 @default.
- W2989025252 hasAuthorship W2989025252A5085173358 @default.
- W2989025252 hasBestOaLocation W29890252522 @default.
- W2989025252 hasConcept C120665830 @default.
- W2989025252 hasConcept C121332964 @default.
- W2989025252 hasConcept C12554922 @default.
- W2989025252 hasConcept C16685009 @default.
- W2989025252 hasConcept C185592680 @default.
- W2989025252 hasConcept C196843134 @default.
- W2989025252 hasConcept C202751555 @default.
- W2989025252 hasConcept C2775920511 @default.
- W2989025252 hasConcept C2776690073 @default.
- W2989025252 hasConcept C28406088 @default.
- W2989025252 hasConcept C31332276 @default.
- W2989025252 hasConcept C523546767 @default.
- W2989025252 hasConcept C54355233 @default.
- W2989025252 hasConcept C55493867 @default.
- W2989025252 hasConcept C71924100 @default.
- W2989025252 hasConcept C86803240 @default.
- W2989025252 hasConcept C91881484 @default.
- W2989025252 hasConcept C95444343 @default.
- W2989025252 hasConceptScore W2989025252C120665830 @default.
- W2989025252 hasConceptScore W2989025252C121332964 @default.
- W2989025252 hasConceptScore W2989025252C12554922 @default.
- W2989025252 hasConceptScore W2989025252C16685009 @default.
- W2989025252 hasConceptScore W2989025252C185592680 @default.
- W2989025252 hasConceptScore W2989025252C196843134 @default.
- W2989025252 hasConceptScore W2989025252C202751555 @default.
- W2989025252 hasConceptScore W2989025252C2775920511 @default.
- W2989025252 hasConceptScore W2989025252C2776690073 @default.