FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2989074879> ?p ?o ?g. }

• W2989074879 endingPage "709" @default.
• W2989074879 startingPage "697" @default.
• W2989074879 abstract "Aims The identification and differentiation of antibiotic-resistant bacteria by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) profiling remains a challenge due to the difficulty in detecting unique protein biomarkers associated with this trait. To expand the detectable proteome in antibiotic-resistant bacteria, we describe a method implementing offline LC protein separation/fractionation prior to MALDI-ToF-MS and top-down MALDI-ToF/ToF-MS (tandem MS or MS/MS) for the analysis of several antibiotic-resistant Escherichia coli isolates. Methods and Results Coupling offline LC with MALDI-ToF-MS increased the number of detected protein signals in the typically analyzed mass regions (m/z 3000–20 000) by a factor of 13. Using the developed LC-MALDI-ToF-MS protocol in conjunction with supervised principal components analysis, we detected a protein biomarker at m/z 9355 which correlated to β-lactam resistance among the E. coli bacteria tested. Implementing a top-down MALDI-ToF/ToF-MS approach, the prefractionated protein biomarker was inferred as a DNA-binding HU protein, likely translated from the blaCMY-2 gene (encoding AmpC-type β-lactamase) in the incompatibility plasmid complex A/C (IncA/C). Conclusions Our results demonstrate the utility of LC-MALDI-MS and MS/MS to extend the number of proteins detected and perform MALDI-accessible protein biomarker discovery in microorganisms. Significance and Impact of the Study This outcome is significant since it expands the detectable bacterial proteome via MALDI-ToF-MS." @default.
• W2989074879 created "2019-11-22" @default.
• W2989074879 creator A5017793557 @default.
• W2989074879 creator A5019478578 @default.
• W2989074879 creator A5067964329 @default.
• W2989074879 creator A5076054391 @default.
• W2989074879 date "2019-12-09" @default.
• W2989074879 modified "2023-09-24" @default.
• W2989074879 title "Detection and identification of a protein biomarker in antibiotic‐resistant <i>Escherichia coli</i> using intact protein LC offline MALDI‐MS and MS/MS" @default.
• W2989074879 cites W1960915086 @default.
• W2989074879 cites W1964343066 @default.
• W2989074879 cites W1969213091 @default.
• W2989074879 cites W1980350579 @default.
• W2989074879 cites W1993625157 @default.
• W2989074879 cites W2006481826 @default.
• W2989074879 cites W2015992367 @default.
• W2989074879 cites W2020270531 @default.
• W2989074879 cites W2023491969 @default.
• W2989074879 cites W2028392326 @default.
• W2989074879 cites W2037859787 @default.
• W2989074879 cites W2047679151 @default.
• W2989074879 cites W2058952776 @default.
• W2989074879 cites W2061626863 @default.
• W2989074879 cites W2065795307 @default.
• W2989074879 cites W2066579410 @default.
• W2989074879 cites W2067758630 @default.
• W2989074879 cites W2072455391 @default.
• W2989074879 cites W2074663945 @default.
• W2989074879 cites W2077541254 @default.
• W2989074879 cites W2099001508 @default.
• W2989074879 cites W2105829810 @default.
• W2989074879 cites W2115129497 @default.
• W2989074879 cites W2120645064 @default.
• W2989074879 cites W2126684840 @default.
• W2989074879 cites W2127072069 @default.
• W2989074879 cites W2129955120 @default.
• W2989074879 cites W2134139244 @default.
• W2989074879 cites W2134190791 @default.
• W2989074879 cites W2135790423 @default.
• W2989074879 cites W2142422928 @default.
• W2989074879 cites W2142919108 @default.
• W2989074879 cites W2150197625 @default.
• W2989074879 cites W2152127318 @default.
• W2989074879 cites W2155911157 @default.
• W2989074879 cites W2165055228 @default.
• W2989074879 cites W2165994761 @default.
• W2989074879 cites W2168086802 @default.
• W2989074879 cites W2168409658 @default.
• W2989074879 cites W2338433338 @default.
• W2989074879 cites W2492932366 @default.
• W2989074879 cites W2784079598 @default.
• W2989074879 cites W4206206329 @default.
• W2989074879 doi "https://doi.org/10.1111/jam.14507" @default.
• W2989074879 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7444928" @default.
• W2989074879 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31715076" @default.
• W2989074879 hasPublicationYear "2019" @default.
• W2989074879 type Work @default.
• W2989074879 sameAs 2989074879 @default.
• W2989074879 citedByCount "13" @default.
• W2989074879 countsByYear W29890748792020 @default.
• W2989074879 countsByYear W29890748792021 @default.
• W2989074879 countsByYear W29890748792022 @default.
• W2989074879 countsByYear W29890748792023 @default.
• W2989074879 crossrefType "journal-article" @default.
• W2989074879 hasAuthorship W2989074879A5017793557 @default.
• W2989074879 hasAuthorship W2989074879A5019478578 @default.
• W2989074879 hasAuthorship W2989074879A5067964329 @default.
• W2989074879 hasAuthorship W2989074879A5076054391 @default.
• W2989074879 hasBestOaLocation W29890748792 @default.
• W2989074879 hasConcept C104317684 @default.
• W2989074879 hasConcept C104397665 @default.
• W2989074879 hasConcept C124535831 @default.
• W2989074879 hasConcept C150394285 @default.
• W2989074879 hasConcept C162356407 @default.
• W2989074879 hasConcept C162711632 @default.
• W2989074879 hasConcept C178790620 @default.
• W2989074879 hasConcept C185592680 @default.
• W2989074879 hasConcept C2781197716 @default.
• W2989074879 hasConcept C43617362 @default.
• W2989074879 hasConcept C46111723 @default.
• W2989074879 hasConcept C523546767 @default.
• W2989074879 hasConcept C54355233 @default.
• W2989074879 hasConcept C547475151 @default.
• W2989074879 hasConcept C55493867 @default.
• W2989074879 hasConcept C75280812 @default.
• W2989074879 hasConcept C86803240 @default.
• W2989074879 hasConceptScore W2989074879C104317684 @default.
• W2989074879 hasConceptScore W2989074879C104397665 @default.
• W2989074879 hasConceptScore W2989074879C124535831 @default.
• W2989074879 hasConceptScore W2989074879C150394285 @default.
• W2989074879 hasConceptScore W2989074879C162356407 @default.
• W2989074879 hasConceptScore W2989074879C162711632 @default.
• W2989074879 hasConceptScore W2989074879C178790620 @default.
• W2989074879 hasConceptScore W2989074879C185592680 @default.
• W2989074879 hasConceptScore W2989074879C2781197716 @default.
• W2989074879 hasConceptScore W2989074879C43617362 @default.
• W2989074879 hasConceptScore W2989074879C46111723 @default.
• W2989074879 hasConceptScore W2989074879C523546767 @default.

• next