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- W2989370972 abstract "One of the main questions addressed in the Nationale Wetenschapsagenda (NWA) is our ability to construct a synthetic minimal cell from lifeless, individual components. In biology, this challenge can be compared to bringing a man to the moon. Various international consortia now aim to engineer such synthetic cells, among which the Dutch initiative BaSyC that is supported by a NWO gravity program. The work presented in this thesis served as a pilot study for BaSyC, and focused on the construction and growth of a boundary layer (membrane) that should surround the synthetic cell. In living cells, membranes are composed of various phospholipids that spontaneously orient in a bilayer-like structure that separates the inside from the outside of the cell. Here, we describe the construction of a biosynthetic pathway that yields two phospholipid species critical for life. By combining catabolic and anabolic enzymes purified from various bacteria, a cascade-like biosynthetic pathway could be assembled capable of forming phospholipids from simple water-soluble building blocks that are fed to the system. The synthesis of these phospholipids resulted in the growth of a pre-established membrane that not only served as a barrier, but also supported the function of membrane embedded enzymes, including a system that is responsible for the translocation of proteins across the membrane. Sustainable production of phospholipids and growth of membranes, as well as the functional integration of membrane growth and membrane protein function, presents a first but important step towards the construction of synthetic minimal cells." @default.
- W2989370972 created "2019-11-22" @default.
- W2989370972 creator A5015204933 @default.
- W2989370972 date "2019-10-11" @default.
- W2989370972 modified "2023-10-03" @default.
- W2989370972 title "Sustainable membrane biosynthesis for synthetic minimal cells" @default.
- W2989370972 cites W1031578623 @default.
- W2989370972 cites W11547850 @default.
- W2989370972 cites W1484198893 @default.
- W2989370972 cites W1485543948 @default.
- W2989370972 cites W1486852747 @default.
- W2989370972 cites W1495024113 @default.
- W2989370972 cites W1514825360 @default.
- W2989370972 cites W1518505333 @default.
- W2989370972 cites W1520271242 @default.
- W2989370972 cites W1534336925 @default.
- W2989370972 cites W1541184580 @default.
- W2989370972 cites W1541497469 @default.
- W2989370972 cites W1552354282 @default.
- W2989370972 cites W1555008776 @default.
- W2989370972 cites W1563955673 @default.
- W2989370972 cites W1564194038 @default.
- W2989370972 cites W1569612573 @default.
- W2989370972 cites W1585341765 @default.
- W2989370972 cites W1587481053 @default.
- W2989370972 cites W1594287794 @default.
- W2989370972 cites W1597921041 @default.
- W2989370972 cites W1607921146 @default.
- W2989370972 cites W1643603004 @default.
- W2989370972 cites W1652876740 @default.
- W2989370972 cites W181452442 @default.
- W2989370972 cites W1816891935 @default.
- W2989370972 cites W1846301815 @default.
- W2989370972 cites W1849927510 @default.
- W2989370972 cites W1908859527 @default.
- W2989370972 cites W1940257192 @default.
- W2989370972 cites W1952600287 @default.
- W2989370972 cites W1966843959 @default.
- W2989370972 cites W1968636401 @default.
- W2989370972 cites W1968874686 @default.
- W2989370972 cites W1972624067 @default.
- W2989370972 cites W1975993749 @default.
- W2989370972 cites W1976034271 @default.
- W2989370972 cites W1976499671 @default.
- W2989370972 cites W1976577540 @default.
- W2989370972 cites W1979591640 @default.
- W2989370972 cites W1981010770 @default.
- W2989370972 cites W1982225654 @default.
- W2989370972 cites W1984097346 @default.
- W2989370972 cites W1985442179 @default.
- W2989370972 cites W1987445483 @default.
- W2989370972 cites W1992033739 @default.
- W2989370972 cites W1992470956 @default.
- W2989370972 cites W1993562651 @default.
- W2989370972 cites W1994874499 @default.
- W2989370972 cites W1994978452 @default.
- W2989370972 cites W1998777312 @default.
- W2989370972 cites W2002100464 @default.
- W2989370972 cites W2002374533 @default.
- W2989370972 cites W2004189813 @default.
- W2989370972 cites W2004863833 @default.
- W2989370972 cites W2005252032 @default.
- W2989370972 cites W2006921115 @default.
- W2989370972 cites W2008223866 @default.
- W2989370972 cites W2008415333 @default.
- W2989370972 cites W2010201275 @default.
- W2989370972 cites W2010374104 @default.
- W2989370972 cites W2012767138 @default.
- W2989370972 cites W2014625274 @default.
- W2989370972 cites W2020417127 @default.
- W2989370972 cites W2020579993 @default.
- W2989370972 cites W2021027911 @default.
- W2989370972 cites W2023186532 @default.
- W2989370972 cites W2023826879 @default.
- W2989370972 cites W2023956010 @default.
- W2989370972 cites W2027408247 @default.
- W2989370972 cites W2027952437 @default.
- W2989370972 cites W2029265973 @default.
- W2989370972 cites W2030915307 @default.
- W2989370972 cites W2033238750 @default.
- W2989370972 cites W2035595494 @default.
- W2989370972 cites W2038567799 @default.
- W2989370972 cites W2044217069 @default.
- W2989370972 cites W2044483591 @default.
- W2989370972 cites W2047051687 @default.
- W2989370972 cites W2047662989 @default.
- W2989370972 cites W2050339362 @default.
- W2989370972 cites W2050573212 @default.
- W2989370972 cites W2051636768 @default.
- W2989370972 cites W2056882263 @default.
- W2989370972 cites W2056891601 @default.
- W2989370972 cites W2057251045 @default.
- W2989370972 cites W2057477511 @default.
- W2989370972 cites W2059013803 @default.
- W2989370972 cites W2066919614 @default.
- W2989370972 cites W2068578978 @default.
- W2989370972 cites W2070898798 @default.
- W2989370972 cites W2071865201 @default.
- W2989370972 cites W2073075045 @default.
- W2989370972 cites W2073726493 @default.