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• W2989403646 abstract "Abstract Background Osteoarthritis (OA) is characterized by degeneration of articular cartilage. Studies have found that enhancement of autophagy, an intracellular catabolic process, may limit the pathologic progression of OA. Chloramphenicol is a potent activator of autophagy; however, the effects of chloramphenicol on articular cartilage are unknown. Questions/purposes Using human OA knee chondrocytes in vitro, we asked, does chloramphenicol (1) activate autophagy in chondrocytes; (2) protect chondrocytes from IL-1β-induced apoptosis; and (3) reduce the expression of matrix metallopeptidase (MMP)-13 and IL-6 (markers associated with articular cartilage degradation and joint inflammation). Using an in vivo rabbit model of OA, we asked, does an intra-articular injection of chloramphenicol in the knee (4) induce autophagy; (5) reduce OA severity; and (6) reduce MMP-13 expression? Methods Human chondrocytes were extracted from 10 men with OA undergoing TKA. After treatment with 25 μg/mL, 50 μg/mL, or 100μg/mL chloramphenicol, the autophagy of chondrocytes was detected with Western blotting, transmission electron microscopy, or an autophagy detection kit. There were four groups in our study: one group was untreated, one was treated with 100 μg/mL chloramphenicol, another was treated with 10 ng/mL of IL-1β, and the final group was treated with 10 ng/mL of IL-1β and 100 μg/mL of chloramphenicol. All groups were treated for 48 hours; cell apoptosis was detected with Western blotting and flow cytometry. Inflammation marker IL-6 in the cell culture supernatant was detected with an ELISA. Articular cartilage degradation-related enzyme MMP-13 was analyzed with Western blotting. A rabbit model of OA was induced by intra-articular injection of type II collagenase in 20 male 3-month-old New Zealand White rabbits' right hind leg knees; the left hind leg knees served as controls. Rabbits were treated by intra-articular injection of saline or chloramphenicol once a week for 8 weeks. Autophagy of the articular cartilage was detected with Western blotting and transmission electron microscopy. Degeneration of articular cartilage was analyzed with Safranin O-fast green staining and the semi-quantitative index Osteoarthritis Research Society International (OARSI) grading system. Degeneration of articular cartilage was evaluated using the OARSI grading system. The expression of MMP-13 in articular cartilage was detected with immunohistochemistry. Results Chloramphenicol activated autophagy in vitro in the chondrocytes of humans with OA and in an in vivo rabbit model of OA. Chloramphenicol inhibited IL-1-induced apoptosis (flow cytometry results with chloramphenicol, 25.33 ± 3.51%, and without chloramphenicol, 44.00 ± 3.61%, mean difference, 18.67% [95% CI 10.60 to 26.73]; p = 0.003) and the production of proinflammatory cytokine IL-6 (ELISA results, with chloramphenicol, 720.00 ± 96.44 pg/mL, without chloramphenicol, 966.67 ± 85.05 pg/mL; mean difference 74.24 pg/mL [95% CI 39.28 to 454.06]; p = 0.029) in chondrocytes. After chloramphenicol treatment, the severity of cartilage degradation was reduced in the treatment group (OARSI 6.80 ± 2.71) compared with the control group (12.30 ± 2.77), (mean difference 5.50 [95% CI 1.50 to 9.50]; p = 0.013). Furthermore, chloramphenicol treatment also decreased the production of MMP-13 in vitro and in vivo. Conclusions Chloramphenicol reduced the severity of cartilage degradation in a type II collagen-induced rabbit model of OA, which may be related to induction of autophagy and inhibition of MMP-13 and IL-6. Clinical Relevance Our study suggests that an intra-articular injection of chloramphenicol may reduce degeneration of articular cartilage and that induction of autophagy may be a method for treating OA. The animal model we used was type II collagen-induced OA, which was different from idiopathic OA and post-traumatic OA. Therefore, we need to use other types of OA models (idiopathic OA or a surgically induced OA model) to further verify its effect, and the side effects of chloramphenicol also need to be considered, such as myelosuppression." @default.
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• W2989403646 date "2019-10-22" @default.
• W2989403646 modified "2023-09-25" @default.
• W2989403646 title "Intra-articular Injection of Chloramphenicol Reduces Articular Cartilage Degeneration in a Rabbit Model of Osteoarthritis" @default.
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• W2989403646 cites W1551310212 @default.
• W2989403646 cites W1554947160 @default.
• W2989403646 cites W1963315781 @default.
• W2989403646 cites W1965307247 @default.
• W2989403646 cites W1977767269 @default.
• W2989403646 cites W1981399177 @default.
• W2989403646 cites W1983351509 @default.
• W2989403646 cites W1985004392 @default.
• W2989403646 cites W1993990835 @default.
• W2989403646 cites W1999307390 @default.
• W2989403646 cites W2004752183 @default.
• W2989403646 cites W2009928065 @default.
• W2989403646 cites W2014140858 @default.
• W2989403646 cites W202319641 @default.
• W2989403646 cites W2027537340 @default.
• W2989403646 cites W2042814728 @default.
• W2989403646 cites W2042932723 @default.
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• W2989403646 cites W2055361189 @default.
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• W2989403646 cites W2080202564 @default.
• W2989403646 cites W2099427592 @default.
• W2989403646 cites W2101471525 @default.
• W2989403646 cites W2108086395 @default.
• W2989403646 cites W2108888253 @default.
• W2989403646 cites W2109034822 @default.
• W2989403646 cites W2113503012 @default.
• W2989403646 cites W2119418077 @default.
• W2989403646 cites W2127631759 @default.
• W2989403646 cites W2186268084 @default.
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• W2989403646 cites W2338986048 @default.
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• W2989403646 cites W2567357359 @default.
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• W2989403646 doi "https://doi.org/10.1097/corr.0000000000001016" @default.
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