FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2989662905> ?p ?o ?g. }

• W2989662905 abstract "Some Brucella isolates are known to require an increased concentration of CO2 for growth, especially in the case of primary cultures obtained directly from infected animals. Moreover, the different Brucella species and biovars show a characteristic pattern of CO2 requirement, and this trait has been included among the routine typing tests used for species and biovar differentiation. By comparing the differences in gene content among different CO2-dependent and CO2-independent Brucella strains, we have confirmed that carbonic anhydrase (CA) II is the enzyme responsible for this phenotype in all the Brucella strains tested. Brucella species contain two CAs of the β family, CA I and CA II; genetic polymorphisms exist for both of them in different isolates, but only those putatively affecting the activity of CA II correlate with the CO2 requirement of the corresponding isolate. Analysis of these polymorphisms does not allow the determination of CA I functionality, while the polymorphisms in CA II consist of small deletions that cause a frameshift that changes the C-terminus of the protein, probably affecting its dimerization status, essential for the activity. CO2-independent mutants arise easily in vitro, although with a low frequency ranging from 10-6 to 10-10 depending on the strain. These mutants carry compensatory mutations that produce a full-length CA II. At the same time, no change was observed in the sequence coding for CA I. A competitive index assay designed to evaluate the fitness of a CO2-dependent strain compared to its corresponding CO2-independent strain revealed that while there is no significant difference when the bacteria are grown in culture plates, growth in vivo in a mouse model of infection provides a significant advantage to the CO2-dependent strain. This could explain why some Brucella isolates are CO2 dependent in primary isolation. The polymorphism described here also allows the in silico determination of the CO2 requirement status of any Brucella strain." @default.
• W2989662905 created "2019-12-05" @default.
• W2989662905 creator A5000420275 @default.
• W2989662905 creator A5004944363 @default.
• W2989662905 creator A5014507674 @default.
• W2989662905 creator A5020001150 @default.
• W2989662905 creator A5050199516 @default.
• W2989662905 creator A5066824821 @default.
• W2989662905 date "2019-12-10" @default.
• W2989662905 modified "2023-10-03" @default.
• W2989662905 title "Polymorphisms in Brucella Carbonic Anhydrase II Mediate CO2 Dependence and Fitness in vivo" @default.
• W2989662905 cites W1493243104 @default.
• W2989662905 cites W1803102843 @default.
• W2989662905 cites W1965807526 @default.
• W2989662905 cites W1966864970 @default.
• W2989662905 cites W1983219311 @default.
• W2989662905 cites W1993879983 @default.
• W2989662905 cites W1996929743 @default.
• W2989662905 cites W2002584091 @default.
• W2989662905 cites W2006208227 @default.
• W2989662905 cites W2016525828 @default.
• W2989662905 cites W2025291938 @default.
• W2989662905 cites W2031463179 @default.
• W2989662905 cites W2032909659 @default.
• W2989662905 cites W2043207210 @default.
• W2989662905 cites W2051470178 @default.
• W2989662905 cites W2056177496 @default.
• W2989662905 cites W2057327357 @default.
• W2989662905 cites W2057928779 @default.
• W2989662905 cites W2061690705 @default.
• W2989662905 cites W2075012623 @default.
• W2989662905 cites W2081549496 @default.
• W2989662905 cites W2089554660 @default.
• W2989662905 cites W2104988217 @default.
• W2989662905 cites W2108449242 @default.
• W2989662905 cites W2119785299 @default.
• W2989662905 cites W2120889005 @default.
• W2989662905 cites W2124951680 @default.
• W2989662905 cites W2136882867 @default.
• W2989662905 cites W2137015675 @default.
• W2989662905 cites W2145154689 @default.
• W2989662905 cites W2152895327 @default.
• W2989662905 cites W2161651656 @default.
• W2989662905 cites W2170143290 @default.
• W2989662905 cites W2471456962 @default.
• W2989662905 cites W2513506562 @default.
• W2989662905 cites W2524341143 @default.
• W2989662905 cites W2567193360 @default.
• W2989662905 cites W2568548070 @default.
• W2989662905 cites W2769755511 @default.
• W2989662905 cites W2792231238 @default.
• W2989662905 cites W2806600957 @default.
• W2989662905 cites W2889889209 @default.
• W2989662905 cites W2970178408 @default.
• W2989662905 cites W4211040199 @default.
• W2989662905 cites W4255550846 @default.
• W2989662905 doi "https://doi.org/10.3389/fmicb.2019.02751" @default.
• W2989662905 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6915039" @default.
• W2989662905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31921002" @default.
• W2989662905 hasPublicationYear "2019" @default.
• W2989662905 type Work @default.
• W2989662905 sameAs 2989662905 @default.
• W2989662905 citedByCount "6" @default.
• W2989662905 countsByYear W29896629052021 @default.
• W2989662905 countsByYear W29896629052022 @default.
• W2989662905 countsByYear W29896629052023 @default.
• W2989662905 crossrefType "journal-article" @default.
• W2989662905 hasAuthorship W2989662905A5000420275 @default.
• W2989662905 hasAuthorship W2989662905A5004944363 @default.
• W2989662905 hasAuthorship W2989662905A5014507674 @default.
• W2989662905 hasAuthorship W2989662905A5020001150 @default.
• W2989662905 hasAuthorship W2989662905A5050199516 @default.
• W2989662905 hasAuthorship W2989662905A5066824821 @default.
• W2989662905 hasBestOaLocation W29896629051 @default.
• W2989662905 hasConcept C104317684 @default.
• W2989662905 hasConcept C105702510 @default.
• W2989662905 hasConcept C127716648 @default.
• W2989662905 hasConcept C143065580 @default.
• W2989662905 hasConcept C159047783 @default.
• W2989662905 hasConcept C16318435 @default.
• W2989662905 hasConcept C181199279 @default.
• W2989662905 hasConcept C207001950 @default.
• W2989662905 hasConcept C2775914622 @default.
• W2989662905 hasConcept C2778022156 @default.
• W2989662905 hasConcept C2778579951 @default.
• W2989662905 hasConcept C2778967756 @default.
• W2989662905 hasConcept C2780767784 @default.
• W2989662905 hasConcept C2781429187 @default.
• W2989662905 hasConcept C29906990 @default.
• W2989662905 hasConcept C54355233 @default.
• W2989662905 hasConcept C55493867 @default.
• W2989662905 hasConcept C86803240 @default.
• W2989662905 hasConcept C89423630 @default.
• W2989662905 hasConceptScore W2989662905C104317684 @default.
• W2989662905 hasConceptScore W2989662905C105702510 @default.
• W2989662905 hasConceptScore W2989662905C127716648 @default.
• W2989662905 hasConceptScore W2989662905C143065580 @default.
• W2989662905 hasConceptScore W2989662905C159047783 @default.
• W2989662905 hasConceptScore W2989662905C16318435 @default.
• W2989662905 hasConceptScore W2989662905C181199279 @default.

• next