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- W2989774047 abstract "Abstract Cyclodextrin-modified micellar ultra pressure liquid chromatography (CD-MUPLC) was firstly developed and directly applied to the simultaneous determination of water-soluble vitamins thiamine hydrochloride (VB1), pyridoxine hydrochloride (VB6) and ascorbic acid (VC) in milk samples. A hybrid isocratic mobile phase consisting of β-cyclodextrin (β-CD, 5.0 mmol L−1) and cetyltrimethylammonium bromide (CTAB, 0.1 mol L−1) in the presence of acetic acid (0.1 mol L−1) at pH 2.9 on a RP-C18 column at 25.0°C was successfully used. The separation of vitamins was achieved in less than 10 min at a 0.2 mL min−1 flow rate showing adequate linearity at 245 nm in the ranges of 5.0–500.0 μg L−1 for VB1, 5.0–1000.0 μg L−1 for VB6 and 5.0–10000.0 μg L−1 for VC with coefficients of variation (r2) of 0.9999, 0.9987 and 0.9971, respectively. In addition, limits of detection obtained were 0.885, 1.352 and 1.358 μg L−1 and limits of quantification were 2.681, 4.096 and 4.115 μg L−1 for VB1, VB6 and VC, respectively. The high sensitivity of the proposed CD-MUPLC-UV method permitted its applications to the determination of water-soluble vitamins VB1 (32–488 μg L−1), VB6 (82–95 μg L−1) and VC (790–45000 μg L−1) in breast and bovine milk samples. The relative standard deviations and recoveries ranged between 0.07 and 2.14% and between 85.27 and 114.8%, respectively, indicating the accurate and precise measurements without any negative impact of matrix. The current analytical method illustrated several advantages including direct, sensitive, selective and non-consuming organic solvents over the hitherto published methods. These features could be attributed to the four-point competitive interactions among analytes, pseudostationary phases and modified C18 stationary phases." @default.
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- W2989774047 date "2019-11-26" @default.
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- W2989774047 title "Cyclodextrin-Modified Micellar UPLC for Direct, Sensitive and Selective Determination of Water Soluble Vitamins in Milk" @default.
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- W2989774047 doi "https://doi.org/10.1093/chromsci/bmz090" @default.
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