Matches in SemOpenAlex for { <https://semopenalex.org/work/W2989787547> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2989787547 abstract "Background and Methods: Human airway trypsin-like protease (HAT) was isolated from the mucoid sputum in airway diseases like bronchial asthma and have indicated that HAT achieves its biological effects via activation of protease activated receptor (PAR)2 in several airway cells. In this work, we examined whether HAT can activate PAR receptors in human primary bronchial epithelial cells (HBECs) by transfecting siRNA of PAR1, PAR2 and PAR3 into HBECs, real-time PCR and immunofluorescence. Results: 1) In control siRNA transfected-HBECs, HAT significantly enhanced expression of PAR1, PAR2, PAR3, and IL-8 mRNAs, PAR1 agonist peptide (PAR1 AP) enhanced PAR1, PAR2 and IL-8 mRNAs, and PAR2 AP enhanced PAR2 and IL-8 mRNAs, but did not enhance PAR1 mRNA. 2) In PAR2 siRNA-transfected HBECs, HAT-induced PAR1, PAR2, PAR3 and IL-8 mRNA elevations and PAR2 AP-induced PAR2 and IL-8 mRNA elevations were almost completely suppressed. 3) In the PAR3 siRNA-transfected HBECs, HAT-induced PAR3 and IL-8 mRNAs were completely suppressed, but PAR1 AP-induced PAR1 mRNA elevation and PAR2 AP-induced PAR2 mRNA were not significantly suppressed. 4) In PAR1 siRNA-transfected HBECs, the PAR1-induced PAR1, PAR2 mRNA elevation were completely suppressed, and both HAT-induced PAR2 mRNA and IL-8 mRNA elevation also suppressed. Immunofluorescence methods showed that PAR2 and PAR3 were localized to the cell surface of HBECs and were internalized by stimulation of HAT. Conclusions: These results indicate that HAT may attack and activate PAR1, PAR2 and PAR3 in HBECs. These PARs, particularly PAR2 and PAR3, are intimately associated with IL-8 synthesis in HBECs, by mutual interrelations." @default.
- W2989787547 created "2019-12-05" @default.
- W2989787547 creator A5015229005 @default.
- W2989787547 creator A5037899102 @default.
- W2989787547 creator A5059674666 @default.
- W2989787547 creator A5080656304 @default.
- W2989787547 creator A5082347184 @default.
- W2989787547 creator A5089304690 @default.
- W2989787547 date "2019-09-28" @default.
- W2989787547 modified "2023-09-27" @default.
- W2989787547 title "Human airway trypsin-like protease stimulates protease activated receptors and interactions of these receptors" @default.
- W2989787547 doi "https://doi.org/10.1183/13993003.congress-2019.pa4090" @default.
- W2989787547 hasPublicationYear "2019" @default.
- W2989787547 type Work @default.
- W2989787547 sameAs 2989787547 @default.
- W2989787547 citedByCount "0" @default.
- W2989787547 crossrefType "proceedings-article" @default.
- W2989787547 hasAuthorship W2989787547A5015229005 @default.
- W2989787547 hasAuthorship W2989787547A5037899102 @default.
- W2989787547 hasAuthorship W2989787547A5059674666 @default.
- W2989787547 hasAuthorship W2989787547A5080656304 @default.
- W2989787547 hasAuthorship W2989787547A5082347184 @default.
- W2989787547 hasAuthorship W2989787547A5089304690 @default.
- W2989787547 hasConcept C104317684 @default.
- W2989787547 hasConcept C105580179 @default.
- W2989787547 hasConcept C125268873 @default.
- W2989787547 hasConcept C149577978 @default.
- W2989787547 hasConcept C153911025 @default.
- W2989787547 hasConcept C170493617 @default.
- W2989787547 hasConcept C181199279 @default.
- W2989787547 hasConcept C203014093 @default.
- W2989787547 hasConcept C2776714187 @default.
- W2989787547 hasConcept C2777292125 @default.
- W2989787547 hasConcept C54009773 @default.
- W2989787547 hasConcept C54355233 @default.
- W2989787547 hasConcept C55493867 @default.
- W2989787547 hasConcept C75184817 @default.
- W2989787547 hasConcept C81885089 @default.
- W2989787547 hasConcept C86803240 @default.
- W2989787547 hasConcept C89560881 @default.
- W2989787547 hasConcept C95444343 @default.
- W2989787547 hasConceptScore W2989787547C104317684 @default.
- W2989787547 hasConceptScore W2989787547C105580179 @default.
- W2989787547 hasConceptScore W2989787547C125268873 @default.
- W2989787547 hasConceptScore W2989787547C149577978 @default.
- W2989787547 hasConceptScore W2989787547C153911025 @default.
- W2989787547 hasConceptScore W2989787547C170493617 @default.
- W2989787547 hasConceptScore W2989787547C181199279 @default.
- W2989787547 hasConceptScore W2989787547C203014093 @default.
- W2989787547 hasConceptScore W2989787547C2776714187 @default.
- W2989787547 hasConceptScore W2989787547C2777292125 @default.
- W2989787547 hasConceptScore W2989787547C54009773 @default.
- W2989787547 hasConceptScore W2989787547C54355233 @default.
- W2989787547 hasConceptScore W2989787547C55493867 @default.
- W2989787547 hasConceptScore W2989787547C75184817 @default.
- W2989787547 hasConceptScore W2989787547C81885089 @default.
- W2989787547 hasConceptScore W2989787547C86803240 @default.
- W2989787547 hasConceptScore W2989787547C89560881 @default.
- W2989787547 hasConceptScore W2989787547C95444343 @default.
- W2989787547 hasLocation W29897875471 @default.
- W2989787547 hasOpenAccess W2989787547 @default.
- W2989787547 hasPrimaryLocation W29897875471 @default.
- W2989787547 hasRelatedWork W1972160241 @default.
- W2989787547 hasRelatedWork W1990155987 @default.
- W2989787547 hasRelatedWork W1994684158 @default.
- W2989787547 hasRelatedWork W2015011901 @default.
- W2989787547 hasRelatedWork W2035964953 @default.
- W2989787547 hasRelatedWork W2045866508 @default.
- W2989787547 hasRelatedWork W2080678631 @default.
- W2989787547 hasRelatedWork W2085624690 @default.
- W2989787547 hasRelatedWork W2088625991 @default.
- W2989787547 hasRelatedWork W2135599949 @default.
- W2989787547 hasRelatedWork W2158343448 @default.
- W2989787547 hasRelatedWork W2164402028 @default.
- W2989787547 hasRelatedWork W2317459888 @default.
- W2989787547 hasRelatedWork W2372462022 @default.
- W2989787547 hasRelatedWork W2595546980 @default.
- W2989787547 hasRelatedWork W2754820021 @default.
- W2989787547 hasRelatedWork W2912987259 @default.
- W2989787547 hasRelatedWork W3030363155 @default.
- W2989787547 hasRelatedWork W3199539825 @default.
- W2989787547 hasRelatedWork W3209310016 @default.
- W2989787547 isParatext "false" @default.
- W2989787547 isRetracted "false" @default.
- W2989787547 magId "2989787547" @default.
- W2989787547 workType "article" @default.