FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2989822644> ?p ?o ?g. }

• W2989822644 endingPage "60" @default.
• W2989822644 startingPage "45" @default.
• W2989822644 abstract "Resonance assignments are challenging for membrane proteins due to the size of the lipid/detergent-protein complex and the presence of line-broadening from conformational exchange. As a consequence, many correlations are missing in the triple-resonance NMR experiments typically used for assignments. Herein, we present an approach in which correlations from these solution-state NMR experiments are supplemented by data from 13C unlabeling, single-amino acid type labeling, 4D NOESY data and proximity of moieties to lipids or water in combination with a structure of the protein. These additional data are used to edit the expected peaklists for the automated assignment protocol FLYA, a module of the program package CYANA. We demonstrate application of the protocol to the 262-residue proton pump from archaeal bacteriorhodopsin (bR) in lipid nanodiscs. The lipid-protein assembly is characterized by an overall correlation time of 44 ns. The protocol yielded assignments for 62% of all backbone (H, N, Cα, Cβ, C') resonances of bR, corresponding to 74% of all observed backbone spin systems, and 60% of the Ala, Met, Ile (δ1), Leu and Val methyl groups, thus enabling to assign a large fraction of the protein without mutagenesis data. Most missing resonances stem from the extracellular half, likely due intermediate exchange line-broadening. Further analysis revealed that missing information of the amino acid type of the preceding residue is the largest problem, and that 4D NOESY experiments are particularly helpful to compensate for that information loss." @default.
• W2989822644 created "2019-12-05" @default.
• W2989822644 creator A5006950257 @default.
• W2989822644 creator A5007360864 @default.
• W2989822644 creator A5049373174 @default.
• W2989822644 creator A5059078778 @default.
• W2989822644 creator A5063892106 @default.
• W2989822644 creator A5083189052 @default.
• W2989822644 creator A5085798885 @default.
• W2989822644 creator A5088501228 @default.
• W2989822644 date "2019-11-21" @default.
• W2989822644 modified "2023-10-16" @default.
• W2989822644 title "Backbone and methyl assignment of bacteriorhodopsin incorporated into nanodiscs" @default.
• W2989822644 cites W1964549109 @default.
• W2989822644 cites W1968827114 @default.
• W2989822644 cites W1971026764 @default.
• W2989822644 cites W1981021420 @default.
• W2989822644 cites W1984484995 @default.
• W2989822644 cites W1984788802 @default.
• W2989822644 cites W1990675281 @default.
• W2989822644 cites W199186236 @default.
• W2989822644 cites W1999768562 @default.
• W2989822644 cites W2004079991 @default.
• W2989822644 cites W2009077514 @default.
• W2989822644 cites W2009973554 @default.
• W2989822644 cites W2014745838 @default.
• W2989822644 cites W2017668510 @default.
• W2989822644 cites W2017710713 @default.
• W2989822644 cites W2019306938 @default.
• W2989822644 cites W2021782267 @default.
• W2989822644 cites W2023490737 @default.
• W2989822644 cites W2034766560 @default.
• W2989822644 cites W2047291465 @default.
• W2989822644 cites W2048775155 @default.
• W2989822644 cites W2048837038 @default.
• W2989822644 cites W2053202752 @default.
• W2989822644 cites W2065868638 @default.
• W2989822644 cites W2066757464 @default.
• W2989822644 cites W2072124763 @default.
• W2989822644 cites W2078892740 @default.
• W2989822644 cites W2091741783 @default.
• W2989822644 cites W2094242581 @default.
• W2989822644 cites W2097692518 @default.
• W2989822644 cites W2112418305 @default.
• W2989822644 cites W2115357061 @default.
• W2989822644 cites W2120373381 @default.
• W2989822644 cites W2122632516 @default.
• W2989822644 cites W2127654342 @default.
• W2989822644 cites W2134976856 @default.
• W2989822644 cites W2137076167 @default.
• W2989822644 cites W2145076926 @default.
• W2989822644 cites W2147281292 @default.
• W2989822644 cites W2148395120 @default.
• W2989822644 cites W2153502397 @default.
• W2989822644 cites W2155350436 @default.
• W2989822644 cites W2155608563 @default.
• W2989822644 cites W2159059233 @default.
• W2989822644 cites W2166025323 @default.
• W2989822644 cites W2169462961 @default.
• W2989822644 cites W2174960973 @default.
• W2989822644 cites W2206954826 @default.
• W2989822644 cites W2316378209 @default.
• W2989822644 cites W2320903312 @default.
• W2989822644 cites W2415222418 @default.
• W2989822644 cites W2476271551 @default.
• W2989822644 cites W2555624997 @default.
• W2989822644 cites W2595870221 @default.
• W2989822644 cites W2735746061 @default.
• W2989822644 cites W2750063845 @default.
• W2989822644 cites W2766230524 @default.
• W2989822644 cites W2769297176 @default.
• W2989822644 cites W2779964566 @default.
• W2989822644 cites W2888788869 @default.
• W2989822644 cites W2940704580 @default.
• W2989822644 cites W2946407865 @default.
• W2989822644 cites W2948073409 @default.
• W2989822644 cites W2950508986 @default.
• W2989822644 cites W68540787 @default.
• W2989822644 doi "https://doi.org/10.1007/s10858-019-00289-7" @default.
• W2989822644 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7015963" @default.
• W2989822644 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31754899" @default.
• W2989822644 hasPublicationYear "2019" @default.
• W2989822644 type Work @default.
• W2989822644 sameAs 2989822644 @default.
• W2989822644 citedByCount "13" @default.
• W2989822644 countsByYear W29898226442020 @default.
• W2989822644 countsByYear W29898226442021 @default.
• W2989822644 countsByYear W29898226442022 @default.
• W2989822644 countsByYear W29898226442023 @default.
• W2989822644 crossrefType "journal-article" @default.
• W2989822644 hasAuthorship W2989822644A5006950257 @default.
• W2989822644 hasAuthorship W2989822644A5007360864 @default.
• W2989822644 hasAuthorship W2989822644A5049373174 @default.
• W2989822644 hasAuthorship W2989822644A5059078778 @default.
• W2989822644 hasAuthorship W2989822644A5063892106 @default.
• W2989822644 hasAuthorship W2989822644A5083189052 @default.
• W2989822644 hasAuthorship W2989822644A5085798885 @default.
• W2989822644 hasAuthorship W2989822644A5088501228 @default.

• next