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• W2989833958 abstract "Purpose MRI‐based cell tracking identifies the location of magnetically labeled cells with hypointense voxels. Here we demonstrate a strain‐dependent effect of liver MRI background on the feasibility of MRI‐based cell tracking of transplanted cells in the mouse liver. Methods FVB mice (GFP‐LUC and NOG) and C57BL/6 mice (GFP+ and wild‐type) were fed 3 different diets with varying iron content. In vivo ‐weighted images and maps of the liver were acquired at different ages. Magnetically labeled cancer cells were injected intrasplenically for hepatic migration; then, mice were imaged by in vivo MRI and bioluminescence imaging. Livers were also imaged ex vivo by magnetic particle imaging. Results increased with age in FVB NOG and FVB GFP‐LUC mice that were fed diets sufficient in iron. FVB NOG mice developed a mottled appearance in their livers with age that did not occur in FVB GFP‐LUC mice. was unchanging with age in C57BL/6 GFP mice, and the liver remained bright and homogenous. Labeled cells were not detectable by MRI in some livers despite successful engraftment as shown by bioluminescence imaging and magnetic particle imaging. Conclusion Strain, diet, and age are important considerations for MRI‐based cell tracking in the liver. If a model with excessive liver iron must be used, alternative imaging methods such as magnetic particle imaging can be considered." @default.
• W2989833958 created "2019-12-05" @default.
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• W2989833958 date "2019-11-25" @default.
• W2989833958 modified "2023-10-14" @default.
• W2989833958 title "Effect of mouse strain and diet on feasibility of MRI‐based cell tracking in the liver" @default.
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• W2989833958 doi "https://doi.org/10.1002/mrm.28081" @default.
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