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- W2990148059 abstract "Abstract Environmental applications of synthetic biology such as water remediation require engineered strains to function robustly in a fluctuating and potentially hostile environment. The construction of synthetic biofilm formation circuits could potentially alleviate this issue by promoting cell survival. Towards this end, we construct a xylose-inducible system for the expression of the functional amyloids CsgA and TasA in the soil bacterium Bacillus megaterium . We find that although both amyloids are expressed, only TasA is successfully exported from the cells. Furthermore, expression of CsgA results in a significant growth penalty for the cells while expression of TasA does not. Finally, we show that TasA expression conveys a small but detectable increase in cells’ adhesion to nickel beads. These results suggest that TasA is a promising candidate for future work on synthetic biofilm formation in B. megaterium ." @default.
- W2990148059 created "2019-12-05" @default.
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- W2990148059 date "2019-11-29" @default.
- W2990148059 modified "2023-09-23" @default.
- W2990148059 title "Construction of an inducible amyloid expression circuit in <i>Bacillus megaterium:</i> A case study with CsgA and TasA" @default.
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- W2990148059 doi "https://doi.org/10.1101/858266" @default.
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