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- W2990274361 abstract "105 Mitochondria, the power houses of the cell, are at the cross-road of many cellular pathways. They play central roles in energy metabolism, maintenance of ion homeostasis and redox balance, and regulation of apoptosis. Mitochondrial DNA (mtDNA) mutations are involved in a variety of pathologies including neurodegenerative diseases, cancer and aging. Here we report the inducible expression of a dominant negative form of the mitochondrial replication enzyme, DNA polymerase (pol γ), in cell culture and the biological consequence. T-ReX293 cells were transfected with a plasmid contains a D1153A substitution in the pol γ gene that encodes a dominant negative form of the enzyme (POLGdn). Severe mitochondrial dysfunction was observed upon the induction of the transgene expression. A decrease in mtDNA content was detected by PCR analysis, and suppressed mtDNA replication activity was revealed by an in vitro replication assay. The mitochondrial encoded protein cytochrome c oxidase II (COII) was substantially reduced at day 6 of the transgene expression, and became undetectable within two weeks. By day 13 of the transgene induction, the cells showed ρ° phenol type, characterized by a lack of electron transport chain activity and deficiency in respiration, auxotrophic for pyruvate and uridine, and a decrease in superoxide radical generation. The cells became dependent on glycolysis and exhibited increased glucose uptake and enhanced lactate production. Mitochondrial mass was increased in cells expressing POLGdn. Interestingly, a concurrent reduction of nucleus-encoded protein APE, cyclophilin D and an elevation of HKII protein were also observed. The data indicate that expression of POLGdn not only caused mitochondrial dysfunction but also affected the nucleus encoded gene expression, leading to profound change in cellular functions. This inducible POLGdn expression system provides a unique experimental model to investigate the roles of mitochondrial dysfunction in causing changes in nuclear gene expression and cellular behaviors, and alterations in apoptosis and cellular response to anticancer drugs." @default.
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- W2990274361 date "2006-04-15" @default.
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- W2990274361 title "Inducible expression of dominant negative DNA polymerase gamma(POLGdn) in human cells: characterizations and applications" @default.
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