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- W2990291230 abstract "Monoclonal antibodies are conquering the biopharmaceutical market because they can be used to treat a variety of diseases. Therefore, it is very important to establish robust and optimized processes for their production. In this article, the first step of chromatography (Protein A chromatography) in monoclonal antibody purification was optimized with a focus on the critical elution step. Therefore, different buffers (citrate, glycine, acetate) were tested for chromatographic performance and product quality. Membrane chromatography was evaluated because it promises high throughputs and short cycle times. The membrane adsorber Sartobind® Protein A 2 mL was used to accelerate the purification procedure and was further used to perform a continuous chromatographic run with a four-membrane adsorber-periodic counter-current chromatography (4MA-PCCC) system. It was found that citrate buffer at pH 3.5 and 0.15 M NaCl enabled the highest recovery of >95% and lowest total aggregate content of 0.26%. In the continuous process, the capacity utilization of the membrane adsorber was increased by 20%." @default.
- W2990291230 created "2019-12-05" @default.
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- W2990291230 date "2019-11-27" @default.
- W2990291230 modified "2023-10-11" @default.
- W2990291230 title "Membrane Adsorber for the Fast Purification of a Monoclonal Antibody Using Protein A Chromatography" @default.
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- W2990291230 doi "https://doi.org/10.3390/membranes9120159" @default.
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