FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2990504909> ?p ?o ?g. }

• W2990504909 abstract "Abstract Background Neuraminidase (NA) is a sialidase present, among various locations, in the envelope/membrane of some bacteria/viruses (e.g., influenza virus), and is involved in infectiveness and/or dispersion. The administration of NA within the brain lateral ventricle represents a model of acute sterile inflammation. The relevance of the Toll-like receptors TLR2 and TLR4 (particularly those in microglial cells) in such process was investigated. Methods Mouse strains deficient in either TLR2 (TLR2 -/- ) or TLR4 (TLR4 -/- ) were used. NA was injected in the lateral ventricle, and the inflammatory reaction was studied by immunohistochemistry (IBA1 and IL-1β) and qPCR (cytokine response). Also, microglia was isolated from those strains and in vitro stimulated with NA, or with TLR2/TLR4 agonists as positive controls (P3C and LPS respectively). The relevance of the sialidase activity of NA was investigated by stimulating microglia with heat-inactivated NA, or with native NA in the presence of sialidase inhibitors (oseltamivir phosphate and N-acetyl-2,3-dehydro-2-deoxyneuraminic acid). Results In septofimbria and hypothalamus, IBA1-positive and IL-1β-positive cell counts increased after NA injection in wild type (WT) mice. In TLR4 -/- mice, such increases were largely abolished, while were only slightly diminished in TLR2 -/- mice. Similarly, the NA-induced expression of IL-1β, TNFα, and IL-6 was completely blocked in TLR4 -/- mice, and only partially reduced in TLR2 -/- mice. In isolated cultured microglia, NA induced a cytokine response (IL-1β, TNFα, and IL-6) in WT microglia, but was unable to do so in TLR4 -/- microglia; TLR2 deficiency partially affected the NA-induced microglial response. When WT microglia was exposed in vitro to heat-inactivated NA or to native NA along with sialidase inhibitors, the NA-induced microglia activation was almost completely abrogated. Conclusions NA is able to directly activate microglial cells, and it does so mostly acting through the TLR4 receptor, while TLR2 has a secondary role. Accordingly, the inflammatory reaction induced by NA in vivo is partially dependent on TLR2, while TLR4 plays a crucial role. Also, the sialidase activity of NA is critical for microglial activation. These results highlight the relevance of microbial NA in the neuroinflammation provoked by NA-bearing pathogens and the possibility of targeting its sialidase activity to ameliorate its impact." @default.
• W2990504909 created "2019-12-05" @default.
• W2990504909 creator A5017446338 @default.
• W2990504909 creator A5036579970 @default.
• W2990504909 creator A5062683632 @default.
• W2990504909 creator A5067514241 @default.
• W2990504909 date "2019-12-01" @default.
• W2990504909 modified "2023-10-18" @default.
• W2990504909 title "Microglial activation by microbial neuraminidase through TLR2 and TLR4 receptors" @default.
• W2990504909 cites W1483512866 @default.
• W2990504909 cites W1559052010 @default.
• W2990504909 cites W1565619760 @default.
• W2990504909 cites W1740130847 @default.
• W2990504909 cites W1768846296 @default.
• W2990504909 cites W1798758220 @default.
• W2990504909 cites W1965820060 @default.
• W2990504909 cites W1974728866 @default.
• W2990504909 cites W1978536616 @default.
• W2990504909 cites W1981182624 @default.
• W2990504909 cites W1981408589 @default.
• W2990504909 cites W1989569828 @default.
• W2990504909 cites W1995744610 @default.
• W2990504909 cites W2004313038 @default.
• W2990504909 cites W2015827876 @default.
• W2990504909 cites W2018716110 @default.
• W2990504909 cites W2041886194 @default.
• W2990504909 cites W2045098547 @default.
• W2990504909 cites W2045475619 @default.
• W2990504909 cites W2050046993 @default.
• W2990504909 cites W2050205112 @default.
• W2990504909 cites W2059275657 @default.
• W2990504909 cites W2060633883 @default.
• W2990504909 cites W2064815909 @default.
• W2990504909 cites W2065549997 @default.
• W2990504909 cites W2066730813 @default.
• W2990504909 cites W2067267561 @default.
• W2990504909 cites W2069981713 @default.
• W2990504909 cites W2077019112 @default.
• W2990504909 cites W2079605309 @default.
• W2990504909 cites W2085662515 @default.
• W2990504909 cites W2086358466 @default.
• W2990504909 cites W2086580991 @default.
• W2990504909 cites W2092196742 @default.
• W2990504909 cites W2095598034 @default.
• W2990504909 cites W2096109794 @default.
• W2990504909 cites W2107651145 @default.
• W2990504909 cites W2108244474 @default.
• W2990504909 cites W2110572579 @default.
• W2990504909 cites W2111083760 @default.
• W2990504909 cites W2115515575 @default.
• W2990504909 cites W2122017644 @default.
• W2990504909 cites W2124667416 @default.
• W2990504909 cites W2137003603 @default.
• W2990504909 cites W2142153279 @default.
• W2990504909 cites W2143516276 @default.
• W2990504909 cites W2148387062 @default.
• W2990504909 cites W2154663804 @default.
• W2990504909 cites W2159297309 @default.
• W2990504909 cites W2161208630 @default.
• W2990504909 cites W2163169397 @default.
• W2990504909 cites W2168393359 @default.
• W2990504909 cites W234950505 @default.
• W2990504909 cites W2401873130 @default.
• W2990504909 cites W2590500739 @default.
• W2990504909 cites W2594284639 @default.
• W2990504909 cites W2742827847 @default.
• W2990504909 cites W2745849139 @default.
• W2990504909 cites W4246077736 @default.
• W2990504909 doi "https://doi.org/10.1186/s12974-019-1643-9" @default.
• W2990504909 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6889729" @default.
• W2990504909 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31791382" @default.
• W2990504909 hasPublicationYear "2019" @default.
• W2990504909 type Work @default.
• W2990504909 sameAs 2990504909 @default.
• W2990504909 citedByCount "28" @default.
• W2990504909 countsByYear W29905049092020 @default.
• W2990504909 countsByYear W29905049092021 @default.
• W2990504909 countsByYear W29905049092022 @default.
• W2990504909 countsByYear W29905049092023 @default.
• W2990504909 crossrefType "journal-article" @default.
• W2990504909 hasAuthorship W2990504909A5017446338 @default.
• W2990504909 hasAuthorship W2990504909A5036579970 @default.
• W2990504909 hasAuthorship W2990504909A5062683632 @default.
• W2990504909 hasAuthorship W2990504909A5067514241 @default.
• W2990504909 hasBestOaLocation W29905049091 @default.
• W2990504909 hasConcept C126322002 @default.
• W2990504909 hasConcept C134018914 @default.
• W2990504909 hasConcept C136449434 @default.
• W2990504909 hasConcept C153911025 @default.
• W2990504909 hasConcept C170493617 @default.
• W2990504909 hasConcept C185592680 @default.
• W2990504909 hasConcept C203014093 @default.
• W2990504909 hasConcept C2776070231 @default.
• W2990504909 hasConcept C2776709828 @default.
• W2990504909 hasConcept C2776914184 @default.
• W2990504909 hasConcept C2778690821 @default.
• W2990504909 hasConcept C2779830541 @default.
• W2990504909 hasConcept C2781236682 @default.
• W2990504909 hasConcept C55493867 @default.
• W2990504909 hasConcept C71924100 @default.

• next