FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2990599110> ?p ?o ?g. }

• W2990599110 endingPage "414" @default.
• W2990599110 startingPage "403" @default.
• W2990599110 abstract "Abstract Nanodiscs (NDs) are an excellent alternative to small unilamellar vesicles (SUVs) for studies of membrane protein structure, but it has not yet been shown that membrane proteins are able to spontaneously fold and insert into a solution of freely diffusing NDs. In this article, we present SDS-PAGE differential mobility studies combined with fluorescence, circular dichroism, and ultraviolet resonance Raman spectroscopy to confirm the spontaneous folding of outer membrane protein A (OmpA) into preformed NDs. Folded OmpA in NDs was incubated with Arg-C protease, resulting in the digestion of OmpA to membrane-protected fragments with an apparent molecular mass of ∼26 kDa (major component) and ∼24 kDa (minor component). The OmpA folding yields were greater than 88% in both NDs and SUVs. An OmpA adsorbed intermediate on NDs could be isolated at low temperature and induced to fold via an increase in temperature, analogous to the temperature-jump experiments on SUVs. The circular dichroism spectra of OmpA in NDs and SUVs were similar and indicated β-barrel secondary structure. Further evidence of OmpA folding into NDs was provided by ultraviolet resonance Raman spectroscopy, which revealed the intense 785 cm−1 structural marker for folded OmpA in NDs. The primary difference between folding in NDs and SUVs was the kinetics; the rate of folding was two- to threefold slower in NDs compared to in SUVs, and this decreased rate can tentatively be attributed to the properties of NDs. These data indicate that NDs may be an excellent alternative to SUVs for folding experiments and offer benefits of optical clarity, sample homogeneity, control of ND:protein ratios, and greater stability." @default.
• W2990599110 created "2019-12-05" @default.
• W2990599110 creator A5070590926 @default.
• W2990599110 creator A5090646410 @default.
• W2990599110 creator A5091212016 @default.
• W2990599110 date "2020-01-01" @default.
• W2990599110 modified "2023-09-29" @default.
• W2990599110 title "Folding of the β-Barrel Membrane Protein OmpA into Nanodiscs" @default.
• W2990599110 cites W1584791707 @default.
• W2990599110 cites W165264657 @default.
• W2990599110 cites W1937307250 @default.
• W2990599110 cites W1965802152 @default.
• W2990599110 cites W1974061371 @default.
• W2990599110 cites W1975542665 @default.
• W2990599110 cites W1979387698 @default.
• W2990599110 cites W1985776027 @default.
• W2990599110 cites W1986665696 @default.
• W2990599110 cites W1987024731 @default.
• W2990599110 cites W1992819425 @default.
• W2990599110 cites W1997394451 @default.
• W2990599110 cites W2003712960 @default.
• W2990599110 cites W2004450155 @default.
• W2990599110 cites W2004688141 @default.
• W2990599110 cites W2004696942 @default.
• W2990599110 cites W2008887848 @default.
• W2990599110 cites W2009576890 @default.
• W2990599110 cites W2009581450 @default.
• W2990599110 cites W2011434357 @default.
• W2990599110 cites W2014424890 @default.
• W2990599110 cites W2033309842 @default.
• W2990599110 cites W2033693106 @default.
• W2990599110 cites W2041374005 @default.
• W2990599110 cites W2042473787 @default.
• W2990599110 cites W2045155470 @default.
• W2990599110 cites W2046612518 @default.
• W2990599110 cites W2050448756 @default.
• W2990599110 cites W2050880321 @default.
• W2990599110 cites W2052311523 @default.
• W2990599110 cites W2059182105 @default.
• W2990599110 cites W2061343602 @default.
• W2990599110 cites W2063868052 @default.
• W2990599110 cites W2080655949 @default.
• W2990599110 cites W2083258595 @default.
• W2990599110 cites W2105314627 @default.
• W2990599110 cites W2123067651 @default.
• W2990599110 cites W2129925452 @default.
• W2990599110 cites W2141375922 @default.
• W2990599110 cites W2147650747 @default.
• W2990599110 cites W2163863893 @default.
• W2990599110 cites W2192218325 @default.
• W2990599110 cites W2225375499 @default.
• W2990599110 cites W2302411709 @default.
• W2990599110 cites W2329237464 @default.
• W2990599110 cites W2587450560 @default.
• W2990599110 cites W2621338256 @default.
• W2990599110 cites W2622258449 @default.
• W2990599110 cites W2790536127 @default.
• W2990599110 cites W2840068146 @default.
• W2990599110 cites W2907116351 @default.
• W2990599110 cites W393553704 @default.
• W2990599110 doi "https://doi.org/10.1016/j.bpj.2019.11.3381" @default.
• W2990599110 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6976806" @default.
• W2990599110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31843264" @default.
• W2990599110 hasPublicationYear "2020" @default.
• W2990599110 type Work @default.
• W2990599110 sameAs 2990599110 @default.
• W2990599110 citedByCount "3" @default.
• W2990599110 countsByYear W29905991102021 @default.
• W2990599110 countsByYear W29905991102023 @default.
• W2990599110 crossrefType "journal-article" @default.
• W2990599110 hasAuthorship W2990599110A5070590926 @default.
• W2990599110 hasAuthorship W2990599110A5090646410 @default.
• W2990599110 hasAuthorship W2990599110A5091212016 @default.
• W2990599110 hasBestOaLocation W29905991101 @default.
• W2990599110 hasConcept C119599485 @default.
• W2990599110 hasConcept C12554922 @default.
• W2990599110 hasConcept C127413603 @default.
• W2990599110 hasConcept C159985019 @default.
• W2990599110 hasConcept C172227984 @default.
• W2990599110 hasConcept C185592680 @default.
• W2990599110 hasConcept C192562407 @default.
• W2990599110 hasConcept C204328495 @default.
• W2990599110 hasConcept C2776545253 @default.
• W2990599110 hasConcept C55493867 @default.
• W2990599110 hasConcept C86803240 @default.
• W2990599110 hasConceptScore W2990599110C119599485 @default.
• W2990599110 hasConceptScore W2990599110C12554922 @default.
• W2990599110 hasConceptScore W2990599110C127413603 @default.
• W2990599110 hasConceptScore W2990599110C159985019 @default.
• W2990599110 hasConceptScore W2990599110C172227984 @default.
• W2990599110 hasConceptScore W2990599110C185592680 @default.
• W2990599110 hasConceptScore W2990599110C192562407 @default.
• W2990599110 hasConceptScore W2990599110C204328495 @default.
• W2990599110 hasConceptScore W2990599110C2776545253 @default.
• W2990599110 hasConceptScore W2990599110C55493867 @default.
• W2990599110 hasConceptScore W2990599110C86803240 @default.
• W2990599110 hasFunder F4320332161 @default.
• W2990599110 hasIssue "2" @default.

• next