FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2990644827> ?p ?o ?g. }

• W2990644827 abstract "Oocyte competence is one of the key factors determining the proportion of embryos that develop to the blastocyst stage. There is vast evidence that IVM oocytes exhibit less developmental potential than their invivo counterparts. Here, we tested whether supplementation of three cytokines [FGF2 (40 ngmL−1), LIF (20ngmL−1), and IGF1 (20 ngmL−1), termed FLI] improved oocyte maturation, and as a consequence, preimplantation development of bovine embryos invitro. In the first experiment, cumulus-oocyte complexes (COCs) were collected from abattoir-derived ovaries and placed in maturation medium,±FLI, for 18 to 22h. At the end of maturation, COCs were fertilized with sperm from a single Holstein bull known to have high fertility. After an 18- to 20-h fertilization period, putative zygotes were cultured in synthetic oviductal fluid for 8 days. The number of embryos that underwent at least one cellular division (cleavage) and the number of embryos that developed to the blastocyst stage was recorded on Days 3 and 8 after insemination, respectively. The COCs supplemented with FLI (n=554) and controls (n=534) were evaluated across 5 replicates. There was no difference in the cleavage rate (P&gt;0.05) between the two treatments. Development to the blastocyst stage was higher (P=0.05) for FLI-treated COCs (34.9%±1.96) than for the control group (23.9%±1.96). In a second experiment, COCs (n=204) supplemented±FLI were collected and fixed at 6, 12, 18, and 24h after placement in maturation medium. The number of transzonal projections in the COCs was determined by localization of actin filaments by using confocal microscopy. Data were analysed by ANOVA using the GLM procedure of SAS software (version 9.4; SAS Institute Inc.). The model included treatment, time, and the interaction of treatment×time as fixed effects. There was no difference (P&gt;0.05) in the number of transzonal projections at 6h (166.3±8.6 vs. 143.9±8.8) and 12h (107.8±8.4 vs. 128.3±7.7) between FLI-treated and control COCs. However, FLI-treated COCs had fewer (P&lt;0.05) transzonal projections at 18h (67.9±7.8 vs. 100.1±7.7) and 24h (56.4±7.2 vs. 80.6±7.4) compared with the controls. There was a significant treatment×time interaction (P=0.006). In a third experiment, we tested whether the timing of transzonal projection disassociation affected lipid accumulation in the embryo. Blastocysts (n=59) on Day 8 produced from COCs matured±FLI were collected and lipid content was determined by using Nile Red staining. There was no difference (P&gt;0.05) in lipid content between treatments. Thus, supplementation of maturation medium with FLI accelerates the disassociation of transzonal projections in COCs and improves subsequent embryonic development to the blastocyst stage while having no detectable effect on lipid content. Further research is necessary to understand how these cytokines modulate IVM of bovine oocytes. This project was supported by Food for the 21st Century and the Clifton Murphy scholarship fund." @default.
• W2990644827 created "2019-12-05" @default.
• W2990644827 creator A5010157169 @default.
• W2990644827 creator A5047646039 @default.
• W2990644827 creator A5050988587 @default.
• W2990644827 creator A5066185112 @default.
• W2990644827 creator A5080355462 @default.
• W2990644827 date "2020-01-01" @default.
• W2990644827 modified "2023-09-28" @default.
• W2990644827 title "188 Improvement of bovine oocyte maturation invitro through cytokine supplementation" @default.
• W2990644827 doi "https://doi.org/10.1071/rdv32n2ab188" @default.
• W2990644827 hasPublicationYear "2020" @default.
• W2990644827 type Work @default.
• W2990644827 sameAs 2990644827 @default.
• W2990644827 citedByCount "0" @default.
• W2990644827 crossrefType "journal-article" @default.
• W2990644827 hasAuthorship W2990644827A5010157169 @default.
• W2990644827 hasAuthorship W2990644827A5047646039 @default.
• W2990644827 hasAuthorship W2990644827A5050988587 @default.
• W2990644827 hasAuthorship W2990644827A5066185112 @default.
• W2990644827 hasAuthorship W2990644827A5080355462 @default.
• W2990644827 hasConcept C105702510 @default.
• W2990644827 hasConcept C16685009 @default.
• W2990644827 hasConcept C196843134 @default.
• W2990644827 hasConcept C2775966511 @default.
• W2990644827 hasConcept C2776690073 @default.
• W2990644827 hasConcept C2777005246 @default.
• W2990644827 hasConcept C2778177303 @default.
• W2990644827 hasConcept C2781087480 @default.
• W2990644827 hasConcept C28748434 @default.
• W2990644827 hasConcept C512716672 @default.
• W2990644827 hasConcept C54355233 @default.
• W2990644827 hasConcept C71924100 @default.
• W2990644827 hasConcept C86803240 @default.
• W2990644827 hasConcept C87073359 @default.
• W2990644827 hasConcept C88972607 @default.
• W2990644827 hasConceptScore W2990644827C105702510 @default.
• W2990644827 hasConceptScore W2990644827C16685009 @default.
• W2990644827 hasConceptScore W2990644827C196843134 @default.
• W2990644827 hasConceptScore W2990644827C2775966511 @default.
• W2990644827 hasConceptScore W2990644827C2776690073 @default.
• W2990644827 hasConceptScore W2990644827C2777005246 @default.
• W2990644827 hasConceptScore W2990644827C2778177303 @default.
• W2990644827 hasConceptScore W2990644827C2781087480 @default.
• W2990644827 hasConceptScore W2990644827C28748434 @default.
• W2990644827 hasConceptScore W2990644827C512716672 @default.
• W2990644827 hasConceptScore W2990644827C54355233 @default.
• W2990644827 hasConceptScore W2990644827C71924100 @default.
• W2990644827 hasConceptScore W2990644827C86803240 @default.
• W2990644827 hasConceptScore W2990644827C87073359 @default.
• W2990644827 hasConceptScore W2990644827C88972607 @default.
• W2990644827 hasLocation W29906448271 @default.
• W2990644827 hasOpenAccess W2990644827 @default.
• W2990644827 hasPrimaryLocation W29906448271 @default.
• W2990644827 hasRelatedWork W12648440 @default.
• W2990644827 hasRelatedWork W16982084 @default.
• W2990644827 hasRelatedWork W19494045 @default.
• W2990644827 hasRelatedWork W28220017 @default.
• W2990644827 hasRelatedWork W29679740 @default.
• W2990644827 hasRelatedWork W30201213 @default.
• W2990644827 hasRelatedWork W30913171 @default.
• W2990644827 hasRelatedWork W34211087 @default.
• W2990644827 hasRelatedWork W440951 @default.
• W2990644827 hasRelatedWork W5535762 @default.
• W2990644827 isParatext "false" @default.
• W2990644827 isRetracted "false" @default.
• W2990644827 magId "2990644827" @default.
• W2990644827 workType "article" @default.