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- W2991014377 abstract "Abstract Ligand-directed protein labelling allows the introduction of diverse chemical functionalities onto proteins without the need for genetically encoded tags. Here we report a method for the rapid labelling of a protein using a ruthenium-bipyridyl (Ru(II)(bpy) 3 )-modified peptide designed to mimic an interacting BH3 ligand within a BCL-2 family protein-protein interactions. Using sub-stoichiometric quantities of (Ru(II)(bpy) 3 )-modified NOXA-B and irradiation with visible light for 1 min, the anti-apoptotic protein MCL-1 can be photolabelled with a variety of functional tags. In contrast with previous reports on Ru(II)(bpy) 3 -mediated photolabelling, tandem mass spectrometry experiments reveal that the labelling site is a cysteine residue of MCL-1. MCL-1 can be labelled selectively in mixtures with other proteins, including the structurally related BCL-2 member, BCL-x L . These results demonstrate that proximity-induced photolabelling is applicable to interfaces that mediate protein-protein interactions, and pave the way towards future use of ligand-directed proximity labelling for dynamic analysis of the interactome of BCL-2 family proteins." @default.
- W2991014377 created "2019-12-05" @default.
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- W2991014377 date "2019-11-21" @default.
- W2991014377 modified "2023-10-16" @default.
- W2991014377 title "Photocatalytic proximity labelling of MCL-1 by a BH3 ligand" @default.
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- W2991014377 doi "https://doi.org/10.1038/s42004-019-0235-z" @default.
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