FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2991611088> ?p ?o ?g. }

• W2991611088 abstract "Abstract The mitochondrial calcium uniporter (MCU) plays a critical role in the mitochondrial calcium uptake into the matrix. In metazoans, the uniporter is a tightly regulated multi-component system including the pore-forming subunit MCU and several regulators (MICU1, MICU2, EMRE). The calcium-conducting activity of metazoan MCU requires the single-transmembrane protein EMRE. Dictyostelium discoideum (Dd), however, developed a simplified uniporter for which the pore-forming MCU (DdMCU) alone is necessary and sufficient for calcium influx. Here, we report a crystal structure of the N-terminal domain (NTD) of DdMCU at 1.7 Å resolution. The DdMCU-NTD contains four helices and two strands arranged in a fold that is completely different from the known structures of other MCU-NTD homologs. Biochemical and biophysical analyses of DdMCU-NTD in solution indicated that the domain exists as oligomers, most probably as a pentamer or hexamer. Mutagenesis showed that the acidic residues Asp60, Glu72 and Glu74, which appeared to mediate the parallel interface as observed in the crystal structure, participated in the self-assembly of DdMCU-NTD. Intriguingly, the oligomeric complex readily dissociated to lower-order oligomers in the presence of calcium. We propose that the calcium-triggered dissociation of NTD regulates the channel activity of DdMCU by a yet unknown mechanism." @default.
• W2991611088 created "2019-12-05" @default.
• W2991611088 creator A5003012923 @default.
• W2991611088 creator A5011540763 @default.
• W2991611088 creator A5013799554 @default.
• W2991611088 creator A5017666190 @default.
• W2991611088 creator A5029286635 @default.
• W2991611088 creator A5030377405 @default.
• W2991611088 creator A5040389294 @default.
• W2991611088 creator A5065440998 @default.
• W2991611088 creator A5069526821 @default.
• W2991611088 creator A5071924031 @default.
• W2991611088 creator A5085961838 @default.
• W2991611088 date "2019-11-20" @default.
• W2991611088 modified "2023-09-28" @default.
• W2991611088 title "Structural characterization of the N-terminal domain of theDictyostelium discoideummitochondrial calcium uniporter" @default.
• W2991611088 cites W1173363010 @default.
• W2991611088 cites W1616183675 @default.
• W2991611088 cites W1986213783 @default.
• W2991611088 cites W1991055482 @default.
• W2991611088 cites W2002791298 @default.
• W2991611088 cites W2010572143 @default.
• W2991611088 cites W2028452582 @default.
• W2991611088 cites W2030825961 @default.
• W2991611088 cites W2044021980 @default.
• W2991611088 cites W2058521767 @default.
• W2991611088 cites W2067841051 @default.
• W2991611088 cites W2078179312 @default.
• W2991611088 cites W2083990515 @default.
• W2991611088 cites W2085111073 @default.
• W2991611088 cites W2088481648 @default.
• W2991611088 cites W2097167248 @default.
• W2991611088 cites W2109184569 @default.
• W2991611088 cites W2142294730 @default.
• W2991611088 cites W2142903428 @default.
• W2991611088 cites W2144081223 @default.
• W2991611088 cites W2144171388 @default.
• W2991611088 cites W2147281292 @default.
• W2991611088 cites W2147874841 @default.
• W2991611088 cites W2151059887 @default.
• W2991611088 cites W2154557455 @default.
• W2991611088 cites W2168290086 @default.
• W2991611088 cites W2168974130 @default.
• W2991611088 cites W2169707043 @default.
• W2991611088 cites W2169821755 @default.
• W2991611088 cites W2225852931 @default.
• W2991611088 cites W2343726927 @default.
• W2991611088 cites W2415025662 @default.
• W2991611088 cites W2513627011 @default.
• W2991611088 cites W2603488123 @default.
• W2991611088 cites W2811291792 @default.
• W2991611088 cites W2846941494 @default.
• W2991611088 cites W2877383832 @default.
• W2991611088 cites W2882326707 @default.
• W2991611088 cites W2943983020 @default.
• W2991611088 doi "https://doi.org/10.1101/848002" @default.
• W2991611088 hasPublicationYear "2019" @default.
• W2991611088 type Work @default.
• W2991611088 sameAs 2991611088 @default.
• W2991611088 citedByCount "0" @default.
• W2991611088 crossrefType "posted-content" @default.
• W2991611088 hasAuthorship W2991611088A5003012923 @default.
• W2991611088 hasAuthorship W2991611088A5011540763 @default.
• W2991611088 hasAuthorship W2991611088A5013799554 @default.
• W2991611088 hasAuthorship W2991611088A5017666190 @default.
• W2991611088 hasAuthorship W2991611088A5029286635 @default.
• W2991611088 hasAuthorship W2991611088A5030377405 @default.
• W2991611088 hasAuthorship W2991611088A5040389294 @default.
• W2991611088 hasAuthorship W2991611088A5065440998 @default.
• W2991611088 hasAuthorship W2991611088A5069526821 @default.
• W2991611088 hasAuthorship W2991611088A5071924031 @default.
• W2991611088 hasAuthorship W2991611088A5085961838 @default.
• W2991611088 hasBestOaLocation W29916110881 @default.
• W2991611088 hasConcept C104292427 @default.
• W2991611088 hasConcept C104317684 @default.
• W2991611088 hasConcept C12554922 @default.
• W2991611088 hasConcept C178790620 @default.
• W2991611088 hasConcept C181199279 @default.
• W2991611088 hasConcept C185592680 @default.
• W2991611088 hasConcept C189754071 @default.
• W2991611088 hasConcept C2777331810 @default.
• W2991611088 hasConcept C35341161 @default.
• W2991611088 hasConcept C519063684 @default.
• W2991611088 hasConcept C55493867 @default.
• W2991611088 hasConcept C86803240 @default.
• W2991611088 hasConcept C95444343 @default.
• W2991611088 hasConcept C98539663 @default.
• W2991611088 hasConceptScore W2991611088C104292427 @default.
• W2991611088 hasConceptScore W2991611088C104317684 @default.
• W2991611088 hasConceptScore W2991611088C12554922 @default.
• W2991611088 hasConceptScore W2991611088C178790620 @default.
• W2991611088 hasConceptScore W2991611088C181199279 @default.
• W2991611088 hasConceptScore W2991611088C185592680 @default.
• W2991611088 hasConceptScore W2991611088C189754071 @default.
• W2991611088 hasConceptScore W2991611088C2777331810 @default.
• W2991611088 hasConceptScore W2991611088C35341161 @default.
• W2991611088 hasConceptScore W2991611088C519063684 @default.
• W2991611088 hasConceptScore W2991611088C55493867 @default.
• W2991611088 hasConceptScore W2991611088C86803240 @default.
• W2991611088 hasConceptScore W2991611088C95444343 @default.

• next