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• W2991844255 endingPage "105560" @default.
• W2991844255 startingPage "105560" @default.
• W2991844255 abstract "Progesterone receptor isoforms A and B exert different biological effects in breast cancer cells. Alteration of PRA/PRB ratio is often observed during breast cancer progression. High PRA/PRB ratios in breast cancer patients are associated with resistance to chemotherapy and poor prognosis. While it is well accepted that PRA and PRB regulate different sets of genes, how the expression of PRA and PRB alters breast cancer proteomes has not been fully investigated. To directly investigate the effects of PR isoform expression on the breast cancer proteome, both in the presence and absence of progestin, PRA and PRB were independently stably expressed in T47DC42 PR-null breast cancer cells using a doxycycline (Dox)-regulated promoter. Dox induction dose-dependently increased PRA and PRB expression. Dox-induced PRA and PRB showed normal receptor localization and were transcriptionally active. Differential quantitative proteomic analysis by stable isotope dimethyl labeling was performed to quantitatively examine how PR isoforms altered global breast cancer proteomes. Cells expressing PRA in the absence of progestin were enriched in proteins involved in the TCA cycle and enriched in proteins involved in glycolysis in the presence of progestin, whilst cells expressing PRB in the absence and presence progestin were significantly enriched in proteins involved in the cell cycle and cell apoptosis pathways. This proteomic data revealed a link between PR isoform expression and alteration in cell metabolism, cell proliferation, and apoptosis. The enrichment of proteins involved in the glycolytic pathway in breast cancer cells expressing PRA is consistent with stem cell-like properties, previously reported in PRA-rich breast cancer cells. Moreover, compared to liganded PRB, liganded PRA differentially upregulated proteins involved in chromatin remodeling, such as linker histone H1.2. Silencing H1.2 gene expression suppressed PRA-mediated cell proliferation and promoted G2/M and S phase entry of the cell cycle. Additionally, liganded PRA upregulated the expression of cathepsin D (CTSD) protease, whose expression is associated with poor prognosis in breast cancer patients. Together, our data demonstrated that the expression of PRA or PRB dramatically and differentially altered breast cancer cell proteomes. These isoform-specific changes in the breast cancer proteome will help to explain the distinct phenotypic properties of breast cancer cells expressing different levels of PRA and PRB." @default.
• W2991844255 created "2019-12-13" @default.
• W2991844255 creator A5002030977 @default.
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• W2991844255 date "2020-04-01" @default.
• W2991844255 modified "2023-09-24" @default.
• W2991844255 title "Differential quantitative proteomics reveals key proteins related to phenotypic changes of breast cancer cells expressing progesterone receptor A" @default.
• W2991844255 cites W1567018500 @default.
• W2991844255 cites W1569704074 @default.
• W2991844255 cites W1964490749 @default.
• W2991844255 cites W1965341356 @default.
• W2991844255 cites W1965768287 @default.
• W2991844255 cites W1974301460 @default.
• W2991844255 cites W1981153918 @default.
• W2991844255 cites W1987417875 @default.
• W2991844255 cites W1990379626 @default.
• W2991844255 cites W1994110056 @default.
• W2991844255 cites W1997535745 @default.
• W2991844255 cites W1999127637 @default.
• W2991844255 cites W2004829167 @default.
• W2991844255 cites W2012863911 @default.
• W2991844255 cites W2012904520 @default.
• W2991844255 cites W2017107870 @default.
• W2991844255 cites W2019033192 @default.
• W2991844255 cites W2021880374 @default.
• W2991844255 cites W2025995094 @default.
• W2991844255 cites W2026370277 @default.
• W2991844255 cites W2028133290 @default.
• W2991844255 cites W2030899358 @default.
• W2991844255 cites W2036408537 @default.
• W2991844255 cites W2042845922 @default.
• W2991844255 cites W2045493291 @default.
• W2991844255 cites W2054846759 @default.
• W2991844255 cites W2056294267 @default.
• W2991844255 cites W2057832407 @default.
• W2991844255 cites W2066716403 @default.
• W2991844255 cites W2069824481 @default.
• W2991844255 cites W2076448756 @default.
• W2991844255 cites W2077807043 @default.
• W2991844255 cites W2078232516 @default.
• W2991844255 cites W2078802143 @default.
• W2991844255 cites W2082326948 @default.
• W2991844255 cites W2082559087 @default.
• W2991844255 cites W2083179606 @default.
• W2991844255 cites W2094966013 @default.
• W2991844255 cites W2095447416 @default.
• W2991844255 cites W2099395323 @default.
• W2991844255 cites W2111317895 @default.
• W2991844255 cites W2119525258 @default.
• W2991844255 cites W2120891514 @default.
• W2991844255 cites W2123179063 @default.
• W2991844255 cites W2138053247 @default.
• W2991844255 cites W2141268120 @default.
• W2991844255 cites W2144160911 @default.
• W2991844255 cites W2158761186 @default.
• W2991844255 cites W2197675997 @default.
• W2991844255 cites W2281734632 @default.
• W2991844255 cites W2321570512 @default.
• W2991844255 cites W2326165980 @default.
• W2991844255 cites W2396007914 @default.
• W2991844255 cites W2419037911 @default.
• W2991844255 cites W2461857357 @default.
• W2991844255 cites W2469048362 @default.
• W2991844255 cites W2528752659 @default.
• W2991844255 cites W2531506996 @default.
• W2991844255 cites W2553756300 @default.
• W2991844255 cites W2569913966 @default.
• W2991844255 cites W2586763842 @default.
• W2991844255 cites W2594460083 @default.
• W2991844255 cites W2738647095 @default.
• W2991844255 cites W2745398296 @default.
• W2991844255 cites W2754520699 @default.
• W2991844255 cites W2760036295 @default.
• W2991844255 cites W2770275616 @default.
• W2991844255 cites W2804591825 @default.
• W2991844255 cites W2806142898 @default.
• W2991844255 cites W2807575739 @default.
• W2991844255 cites W2890999095 @default.
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• W2991844255 doi "https://doi.org/10.1016/j.jsbmb.2019.105560" @default.
• W2991844255 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31809870" @default.
• W2991844255 hasPublicationYear "2020" @default.
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