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• W29921343 startingPage "2082" @default.
• W29921343 abstract "Abstract A semiquantitative estimation of human T-lymphotropic virus type I (HTLV-I) integration by peripheral blood mononuclear cells (PBMC) was performed. Genomic DNA samples derived from 134 HTLV-I carriers were subjected to 40 or 60 cycles of the polymerase chain reaction to amplify the pol region of HTLV-I. The HTLV-I genome was detected by dot hybridization using a 32P-labeled oligonucleotide probe for the pol region. The radioactivity of hybridized dot membranes was then counted with an RI Imaging System (Ambis Inc, San Diego, CA) and the HTLV-I genome dose was determined by comparison with standard curve for serially diluted HTLV-I genome-positive DNA. A wide range of variation of HTLV-I genome integration was observed. When the integrated genome dose was calculated as the number of HTLV-I copies per 100 PBMC, 7 carriers (5%) had more than 10 copies, 56 (42%) had 1 to 10 copies, 46 (34%) had 0.1 to 1 copy, and 24 (18%) had less than 0.1 copy. In one sample, the HTLV-I genome was undetectable, which may indicate that the integrated genome was present at less than 0.01 copies per 100 PBMC. Age- or sex-related variations in the distribution of individuals with different HTLV-I genome were rather limited. However, carriers with a high level of the HTLV-I genome were always more than 30 years old and were predominantly male (six of seven)." @default.
• W29921343 created "2016-06-24" @default.
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• W29921343 date "1991-10-15" @default.
• W29921343 modified "2023-10-16" @default.
• W29921343 title "Semiquantitative analysis of integrated genomes of human T-lymphotropic virus type I in asymptomatic virus carriers" @default.
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• W29921343 doi "https://doi.org/10.1182/blood.v78.8.2082.2082" @default.
• W29921343 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1680498" @default.
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