Matches in SemOpenAlex for { <https://semopenalex.org/work/W2992184771> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2992184771 abstract "Alpha-synuclein is a protein involved in several neurodegenerative diseases, such as Parkinson’s, where it has been observed to form amyloid fibrils or Lewy bodies. However, how or to what extent alpha-synuclein is involved is not known. I have in this thesis been conducting experiments in order to design an experimental model system to study how alpha-synuclein interacts with a cell membrane. This system consists of giant unilamellar vesicles (GUVs) made up of various lipid species, including 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE). The vesicles are loaded with a fluorescent dye (fluorescein) which starts to leak out if the membrane becomes permeable. It was observed that GUVs with a diameter exceeding 20 μm could be made of different lipids and filled with fluorescein. The GUVs were photobleached and the movement of fluorescein in and out of them was measured. Upon addition of alpha-synuclein it was observed it caused no detectable structural damage of the GUV membrane during 20 minutes. In summary, I have investigated the steps in setting up a general method of studying protein-lipid interactions, where the most ideal way to monitor GUV membrane permeability was found, with specific parameters regarding lipid concentrations to ensure membrane stability. (Less)" @default.
- W2992184771 created "2019-12-13" @default.
- W2992184771 creator A5077893942 @default.
- W2992184771 date "2019-01-01" @default.
- W2992184771 modified "2023-09-27" @default.
- W2992184771 title "Affinity of Alpha-Synuclein and Lipid Vesicle Interaction" @default.
- W2992184771 hasPublicationYear "2019" @default.
- W2992184771 type Work @default.
- W2992184771 sameAs 2992184771 @default.
- W2992184771 citedByCount "0" @default.
- W2992184771 crossrefType "journal-article" @default.
- W2992184771 hasAuthorship W2992184771A5077893942 @default.
- W2992184771 hasConcept C121332964 @default.
- W2992184771 hasConcept C12554922 @default.
- W2992184771 hasConcept C130316041 @default.
- W2992184771 hasConcept C142724271 @default.
- W2992184771 hasConcept C185592680 @default.
- W2992184771 hasConcept C2778015030 @default.
- W2992184771 hasConcept C2779134260 @default.
- W2992184771 hasConcept C2779734285 @default.
- W2992184771 hasConcept C2780943371 @default.
- W2992184771 hasConcept C2781449126 @default.
- W2992184771 hasConcept C39944091 @default.
- W2992184771 hasConcept C41625074 @default.
- W2992184771 hasConcept C55493867 @default.
- W2992184771 hasConcept C62520636 @default.
- W2992184771 hasConcept C71924100 @default.
- W2992184771 hasConcept C86803240 @default.
- W2992184771 hasConcept C91881484 @default.
- W2992184771 hasConceptScore W2992184771C121332964 @default.
- W2992184771 hasConceptScore W2992184771C12554922 @default.
- W2992184771 hasConceptScore W2992184771C130316041 @default.
- W2992184771 hasConceptScore W2992184771C142724271 @default.
- W2992184771 hasConceptScore W2992184771C185592680 @default.
- W2992184771 hasConceptScore W2992184771C2778015030 @default.
- W2992184771 hasConceptScore W2992184771C2779134260 @default.
- W2992184771 hasConceptScore W2992184771C2779734285 @default.
- W2992184771 hasConceptScore W2992184771C2780943371 @default.
- W2992184771 hasConceptScore W2992184771C2781449126 @default.
- W2992184771 hasConceptScore W2992184771C39944091 @default.
- W2992184771 hasConceptScore W2992184771C41625074 @default.
- W2992184771 hasConceptScore W2992184771C55493867 @default.
- W2992184771 hasConceptScore W2992184771C62520636 @default.
- W2992184771 hasConceptScore W2992184771C71924100 @default.
- W2992184771 hasConceptScore W2992184771C86803240 @default.
- W2992184771 hasConceptScore W2992184771C91881484 @default.
- W2992184771 hasLocation W29921847711 @default.
- W2992184771 hasOpenAccess W2992184771 @default.
- W2992184771 hasPrimaryLocation W29921847711 @default.
- W2992184771 hasRelatedWork W1455259219 @default.
- W2992184771 hasRelatedWork W1576697606 @default.
- W2992184771 hasRelatedWork W1808569818 @default.
- W2992184771 hasRelatedWork W1991826033 @default.
- W2992184771 hasRelatedWork W2020884457 @default.
- W2992184771 hasRelatedWork W2024223544 @default.
- W2992184771 hasRelatedWork W2039411159 @default.
- W2992184771 hasRelatedWork W2043358632 @default.
- W2992184771 hasRelatedWork W2075518645 @default.
- W2992184771 hasRelatedWork W2081148902 @default.
- W2992184771 hasRelatedWork W2108001717 @default.
- W2992184771 hasRelatedWork W2146439388 @default.
- W2992184771 hasRelatedWork W2148680997 @default.
- W2992184771 hasRelatedWork W2153157912 @default.
- W2992184771 hasRelatedWork W2186767169 @default.
- W2992184771 hasRelatedWork W2395091435 @default.
- W2992184771 hasRelatedWork W2560541065 @default.
- W2992184771 hasRelatedWork W2767553597 @default.
- W2992184771 hasRelatedWork W3133358203 @default.
- W2992184771 hasRelatedWork W3159947327 @default.
- W2992184771 isParatext "false" @default.
- W2992184771 isRetracted "false" @default.
- W2992184771 magId "2992184771" @default.
- W2992184771 workType "article" @default.