FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2993197026> ?p ?o ?g. }

• W2993197026 endingPage "107814" @default.
• W2993197026 startingPage "107814" @default.
• W2993197026 abstract "Giardia duodenalis is one of the most important intestinal parasites globally, especially in children, and in Cuba is the leading cause of chronic paediatric diarrhoea in this population. G. duodenalis is composed of eight genetic groups (or assemblages), two of which (A and B) are apparently zoonotic, occurring in both humans and other animals. However, consensus on the most appropriate genotyping scheme for optimal characterization of G. duodenalis isolates is lacking. In this article we present the results of three descriptive observational studies conducted in Havana, Cuba between 2010 and 2013, with the aim of comparing the results from molecular (PCR) approaches targeting different genes in order to assign with confidence 224 isolates of G. duodenalis to the correct assemblages. In each sub-study, following DNA isolation by the phenol/chloroform/isoamyl alcohol extraction method, PCR targeting the triose phosphate isomerase (tpi) gene was used for molecular characterization, as well as one additional PCR-method targeting another gene or pair of genes. DNA amplification was obtained in 87%, 83%, and 80% in the three sub-studies. Although excellent agreement (kappa index = 1) was recorded between results from some pairs of genes, for other combinations only moderate or substantial agreement was achieved. These results highlight the importance of interpretation of genotyping data, especially when single genetic markers are used. From the results of our studies, PCR targeting a combination of the tpi gene and the intergenic spacer region of rDNA may be a useful approach for the molecular characterization of G. duodenalis isolates." @default.
• W2993197026 created "2019-12-13" @default.
• W2993197026 creator A5000256495 @default.
• W2993197026 creator A5012283927 @default.
• W2993197026 creator A5014865194 @default.
• W2993197026 creator A5023670682 @default.
• W2993197026 creator A5033618596 @default.
• W2993197026 creator A5046670002 @default.
• W2993197026 creator A5053218696 @default.
• W2993197026 creator A5054194070 @default.
• W2993197026 creator A5056361034 @default.
• W2993197026 date "2020-02-01" @default.
• W2993197026 modified "2023-09-27" @default.
• W2993197026 title "Concordance of Giardia duodenalis assemblages determined by different PCR methodologies in three observational studies in Cuba" @default.
• W2993197026 cites W1527595659 @default.
• W2993197026 cites W1968644762 @default.
• W2993197026 cites W1968710639 @default.
• W2993197026 cites W1999166897 @default.
• W2993197026 cites W2001600427 @default.
• W2993197026 cites W2015289159 @default.
• W2993197026 cites W2015400330 @default.
• W2993197026 cites W2019858741 @default.
• W2993197026 cites W2021869123 @default.
• W2993197026 cites W2022752337 @default.
• W2993197026 cites W2035931483 @default.
• W2993197026 cites W2051037737 @default.
• W2993197026 cites W2054053744 @default.
• W2993197026 cites W2058850623 @default.
• W2993197026 cites W2062553257 @default.
• W2993197026 cites W2069976801 @default.
• W2993197026 cites W2075683777 @default.
• W2993197026 cites W2082120087 @default.
• W2993197026 cites W2086930596 @default.
• W2993197026 cites W2089600442 @default.
• W2993197026 cites W2120510546 @default.
• W2993197026 cites W2134036032 @default.
• W2993197026 cites W2147043577 @default.
• W2993197026 cites W2192659091 @default.
• W2993197026 cites W2257721737 @default.
• W2993197026 cites W2491401860 @default.
• W2993197026 cites W2566592218 @default.
• W2993197026 cites W2594536859 @default.
• W2993197026 cites W2870334649 @default.
• W2993197026 cites W2886839934 @default.
• W2993197026 cites W2900650528 @default.
• W2993197026 cites W4236111915 @default.
• W2993197026 doi "https://doi.org/10.1016/j.exppara.2019.107814" @default.
• W2993197026 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31816280" @default.
• W2993197026 hasPublicationYear "2020" @default.
• W2993197026 type Work @default.
• W2993197026 sameAs 2993197026 @default.
• W2993197026 citedByCount "2" @default.
• W2993197026 countsByYear W29931970262021 @default.
• W2993197026 countsByYear W29931970262022 @default.
• W2993197026 crossrefType "journal-article" @default.
• W2993197026 hasAuthorship W2993197026A5000256495 @default.
• W2993197026 hasAuthorship W2993197026A5012283927 @default.
• W2993197026 hasAuthorship W2993197026A5014865194 @default.
• W2993197026 hasAuthorship W2993197026A5023670682 @default.
• W2993197026 hasAuthorship W2993197026A5033618596 @default.
• W2993197026 hasAuthorship W2993197026A5046670002 @default.
• W2993197026 hasAuthorship W2993197026A5053218696 @default.
• W2993197026 hasAuthorship W2993197026A5054194070 @default.
• W2993197026 hasAuthorship W2993197026A5056361034 @default.
• W2993197026 hasBestOaLocation W29931970261 @default.
• W2993197026 hasConcept C104317684 @default.
• W2993197026 hasConcept C135763542 @default.
• W2993197026 hasConcept C144024400 @default.
• W2993197026 hasConcept C149923435 @default.
• W2993197026 hasConcept C159047783 @default.
• W2993197026 hasConcept C2777271458 @default.
• W2993197026 hasConcept C2908647359 @default.
• W2993197026 hasConcept C31467283 @default.
• W2993197026 hasConcept C49105822 @default.
• W2993197026 hasConcept C54355233 @default.
• W2993197026 hasConcept C78063203 @default.
• W2993197026 hasConcept C86803240 @default.
• W2993197026 hasConceptScore W2993197026C104317684 @default.
• W2993197026 hasConceptScore W2993197026C135763542 @default.
• W2993197026 hasConceptScore W2993197026C144024400 @default.
• W2993197026 hasConceptScore W2993197026C149923435 @default.
• W2993197026 hasConceptScore W2993197026C159047783 @default.
• W2993197026 hasConceptScore W2993197026C2777271458 @default.
• W2993197026 hasConceptScore W2993197026C2908647359 @default.
• W2993197026 hasConceptScore W2993197026C31467283 @default.
• W2993197026 hasConceptScore W2993197026C49105822 @default.
• W2993197026 hasConceptScore W2993197026C54355233 @default.
• W2993197026 hasConceptScore W2993197026C78063203 @default.
• W2993197026 hasConceptScore W2993197026C86803240 @default.
• W2993197026 hasFunder F4320322836 @default.
• W2993197026 hasFunder F4320335353 @default.
• W2993197026 hasLocation W29931970261 @default.
• W2993197026 hasLocation W29931970262 @default.
• W2993197026 hasLocation W29931970263 @default.
• W2993197026 hasOpenAccess W2993197026 @default.
• W2993197026 hasPrimaryLocation W29931970261 @default.
• W2993197026 hasRelatedWork W1150796139 @default.
• W2993197026 hasRelatedWork W119708504 @default.

• next