FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2993485258> ?p ?o ?g. }

• W2993485258 endingPage "26504" @default.
• W2993485258 startingPage "26497" @default.
• W2993485258 abstract "The molybdenum storage protein (MoSto) deposits large amounts of molybdenum as polyoxomolybdate clusters in a heterohexameric (αβ)3 cage-like protein complex under ATP consumption. Here, we suggest a unique mechanism for the ATP-powered molybdate pumping process based on X-ray crystallography, cryoelectron microscopy, hydrogen-deuterium exchange mass spectrometry, and mutational studies of MoSto from Azotobacter vinelandii. First, we show that molybdate, ATP, and Mg2+ consecutively bind into the open ATP-binding groove of the β-subunit, which thereafter becomes tightly locked by fixing the previously disordered N-terminal arm of the α-subunit over the β-ATP. Next, we propose a nucleophilic attack of molybdate onto the γ-phosphate of β-ATP, analogous to the similar reaction of the structurally related UMP kinase. The formed instable phosphoric-molybdic anhydride becomes immediately hydrolyzed and, according to the current data, the released and accelerated molybdate is pressed through the cage wall, presumably by turning aside the Metβ149 side chain. A structural comparison between MoSto and UMP kinase provides valuable insight into how an enzyme is converted into a molecular machine during evolution. The postulated direct conversion of chemical energy into kinetic energy via an activating molybdate kinase and an exothermic pyrophosphatase reaction to overcome a proteinous barrier represents a novelty in ATP-fueled biochemistry, because normally, ATP hydrolysis initiates large-scale conformational changes to drive a distant process." @default.
• W2993485258 created "2019-12-13" @default.
• W2993485258 creator A5000745618 @default.
• W2993485258 creator A5005562570 @default.
• W2993485258 creator A5035071340 @default.
• W2993485258 creator A5041207309 @default.
• W2993485258 creator A5071428495 @default.
• W2993485258 creator A5073528993 @default.
• W2993485258 creator A5083445146 @default.
• W2993485258 date "2019-12-06" @default.
• W2993485258 modified "2023-10-10" @default.
• W2993485258 title "Molybdate pumping into the molybdenum storage protein via an ATP-powered piercing mechanism" @default.
• W2993485258 cites W1548269463 @default.
• W2993485258 cites W1924988108 @default.
• W2993485258 cites W1983396218 @default.
• W2993485258 cites W1991535573 @default.
• W2993485258 cites W2000352572 @default.
• W2993485258 cites W2002995532 @default.
• W2993485258 cites W2016787579 @default.
• W2993485258 cites W2026583779 @default.
• W2993485258 cites W2036194106 @default.
• W2993485258 cites W2052095869 @default.
• W2993485258 cites W2059918071 @default.
• W2993485258 cites W2071950147 @default.
• W2993485258 cites W2082814709 @default.
• W2993485258 cites W2084023045 @default.
• W2993485258 cites W2090691537 @default.
• W2993485258 cites W2101713007 @default.
• W2993485258 cites W2104234755 @default.
• W2993485258 cites W2115481724 @default.
• W2993485258 cites W2122263221 @default.
• W2993485258 cites W2131057470 @default.
• W2993485258 cites W2131918208 @default.
• W2993485258 cites W2132629607 @default.
• W2993485258 cites W2134432941 @default.
• W2993485258 cites W2144081223 @default.
• W2993485258 cites W2147932547 @default.
• W2993485258 cites W2148900757 @default.
• W2993485258 cites W2155590507 @default.
• W2993485258 cites W2163341755 @default.
• W2993485258 cites W2213835809 @default.
• W2993485258 cites W2301903195 @default.
• W2993485258 cites W2325627160 @default.
• W2993485258 cites W2327301431 @default.
• W2993485258 cites W2346085858 @default.
• W2993485258 cites W2432427150 @default.
• W2993485258 cites W2593511418 @default.
• W2993485258 cites W2794208885 @default.
• W2993485258 cites W2801456442 @default.
• W2993485258 cites W2892175279 @default.
• W2993485258 cites W2898360243 @default.
• W2993485258 cites W2924581088 @default.
• W2993485258 cites W4248872320 @default.
• W2993485258 doi "https://doi.org/10.1073/pnas.1913031116" @default.
• W2993485258 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6936712" @default.
• W2993485258 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31811022" @default.
• W2993485258 hasPublicationYear "2019" @default.
• W2993485258 type Work @default.
• W2993485258 sameAs 2993485258 @default.
• W2993485258 citedByCount "6" @default.
• W2993485258 countsByYear W29934852582020 @default.
• W2993485258 countsByYear W29934852582022 @default.
• W2993485258 countsByYear W29934852582023 @default.
• W2993485258 crossrefType "journal-article" @default.
• W2993485258 hasAuthorship W2993485258A5000745618 @default.
• W2993485258 hasAuthorship W2993485258A5005562570 @default.
• W2993485258 hasAuthorship W2993485258A5035071340 @default.
• W2993485258 hasAuthorship W2993485258A5041207309 @default.
• W2993485258 hasAuthorship W2993485258A5071428495 @default.
• W2993485258 hasAuthorship W2993485258A5073528993 @default.
• W2993485258 hasAuthorship W2993485258A5083445146 @default.
• W2993485258 hasBestOaLocation W29934852581 @default.
• W2993485258 hasConcept C12554922 @default.
• W2993485258 hasConcept C141280058 @default.
• W2993485258 hasConcept C141315368 @default.
• W2993485258 hasConcept C178790620 @default.
• W2993485258 hasConcept C179104552 @default.
• W2993485258 hasConcept C181199279 @default.
• W2993485258 hasConcept C181440489 @default.
• W2993485258 hasConcept C185592680 @default.
• W2993485258 hasConcept C23265538 @default.
• W2993485258 hasConcept C2775981058 @default.
• W2993485258 hasConcept C2776159390 @default.
• W2993485258 hasConcept C2779564974 @default.
• W2993485258 hasConcept C2780091537 @default.
• W2993485258 hasConcept C537208039 @default.
• W2993485258 hasConcept C549387045 @default.
• W2993485258 hasConcept C55493867 @default.
• W2993485258 hasConcept C8010536 @default.
• W2993485258 hasConcept C86803240 @default.
• W2993485258 hasConceptScore W2993485258C12554922 @default.
• W2993485258 hasConceptScore W2993485258C141280058 @default.
• W2993485258 hasConceptScore W2993485258C141315368 @default.
• W2993485258 hasConceptScore W2993485258C178790620 @default.
• W2993485258 hasConceptScore W2993485258C179104552 @default.
• W2993485258 hasConceptScore W2993485258C181199279 @default.
• W2993485258 hasConceptScore W2993485258C181440489 @default.
• W2993485258 hasConceptScore W2993485258C185592680 @default.

• next