Matches in SemOpenAlex for { <https://semopenalex.org/work/W2993763753> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2993763753 abstract "Abstract Cryptococcus neoformans is an opportunistic human fungal pathogen commonly associated with infection in immunocompromised individuals (e.g., patients with HIV/AIDS). Important virulence determinants include the production of a polysaccharide capsule, melanin, and extracellular enzymes, as well as the ability to grow at 37°C. C. neoformans controls a plethora of host defense and evasion mechanisms to survive during infection and to proliferate within the host, causing meningoencephalitis and death. Traditionally, characterization of C. neoformans under different environmental conditions and stresses has relied on genetic and phenotypic analyses, as well as biochemical assays. However, advances in mass spectrometry instrumentation, sample preparation protocols, and bioinformatic tools and databases promote comprehensive profiling of fungal cellular processes, secretion or protein release into the extracellular environment, and vesicle contents. Moreover, proteomics provides insight into regulatory mechanisms influencing signal transduction cascades and protein complexes or networks through profiling of post‐translational modifications and protein–protein interactions. Given the medical impact of C. neoformans infections and the recent emergence of antifungal‐resistant strains, defining proteins produced in response to unique environments provides an opportunity to uncover antivirulence strategies and alternative therapeutic options to combat infection. Here, we describe culturing and sample preparation of C. neoformans and outline protocols for comprehensively profiling changes in protein abundance within the cellular proteome and secretome. © 2019 by John Wiley & Sons, Inc. Basic Protocol 1 : Growth and sample preparation of Cryptococcus neoformans Basic Protocol 2 : Protein extraction from supernatant Basic Protocol 3 : Protein extraction from cell pellet Basic Protocol 4 : Proteomic profiling and bioinformatics" @default.
- W2993763753 created "2019-12-13" @default.
- W2993763753 creator A5026472200 @default.
- W2993763753 creator A5090295118 @default.
- W2993763753 date "2019-12-01" @default.
- W2993763753 modified "2023-09-27" @default.
- W2993763753 title "Quantitative Proteomic Profiling of <i>Cryptococcus neoformans</i>" @default.
- W2993763753 cites W1912277387 @default.
- W2993763753 cites W1943178454 @default.
- W2993763753 cites W1953102621 @default.
- W2993763753 cites W1974043955 @default.
- W2993763753 cites W1981098361 @default.
- W2993763753 cites W1985214490 @default.
- W2993763753 cites W1986656413 @default.
- W2993763753 cites W2002155770 @default.
- W2993763753 cites W2036321220 @default.
- W2993763753 cites W2048280438 @default.
- W2993763753 cites W2056573634 @default.
- W2993763753 cites W2064631610 @default.
- W2993763753 cites W2065118231 @default.
- W2993763753 cites W2080752012 @default.
- W2993763753 cites W2113905763 @default.
- W2993763753 cites W2132664783 @default.
- W2993763753 cites W2152673223 @default.
- W2993763753 cites W2170776626 @default.
- W2993763753 cites W2171525151 @default.
- W2993763753 cites W2238545098 @default.
- W2993763753 cites W243079712 @default.
- W2993763753 cites W2461588269 @default.
- W2993763753 cites W2463195069 @default.
- W2993763753 cites W2519691041 @default.
- W2993763753 cites W2565480917 @default.
- W2993763753 cites W2611697423 @default.
- W2993763753 cites W2612414000 @default.
- W2993763753 cites W2772184539 @default.
- W2993763753 cites W2804198042 @default.
- W2993763753 cites W2887294942 @default.
- W2993763753 cites W2953080604 @default.
- W2993763753 cites W2976507922 @default.
- W2993763753 cites W3190406687 @default.
- W2993763753 doi "https://doi.org/10.1002/cpmc.94" @default.
- W2993763753 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31797572" @default.
- W2993763753 hasPublicationYear "2019" @default.
- W2993763753 type Work @default.
- W2993763753 sameAs 2993763753 @default.
- W2993763753 citedByCount "12" @default.
- W2993763753 countsByYear W29937637532020 @default.
- W2993763753 countsByYear W29937637532021 @default.
- W2993763753 countsByYear W29937637532022 @default.
- W2993763753 countsByYear W29937637532023 @default.
- W2993763753 crossrefType "journal-article" @default.
- W2993763753 hasAuthorship W2993763753A5026472200 @default.
- W2993763753 hasAuthorship W2993763753A5090295118 @default.
- W2993763753 hasConcept C104317684 @default.
- W2993763753 hasConcept C104397665 @default.
- W2993763753 hasConcept C2777893358 @default.
- W2993763753 hasConcept C2779413141 @default.
- W2993763753 hasConcept C46111723 @default.
- W2993763753 hasConcept C54355233 @default.
- W2993763753 hasConcept C60644358 @default.
- W2993763753 hasConcept C60987743 @default.
- W2993763753 hasConcept C70721500 @default.
- W2993763753 hasConcept C80311884 @default.
- W2993763753 hasConcept C86803240 @default.
- W2993763753 hasConcept C89423630 @default.
- W2993763753 hasConceptScore W2993763753C104317684 @default.
- W2993763753 hasConceptScore W2993763753C104397665 @default.
- W2993763753 hasConceptScore W2993763753C2777893358 @default.
- W2993763753 hasConceptScore W2993763753C2779413141 @default.
- W2993763753 hasConceptScore W2993763753C46111723 @default.
- W2993763753 hasConceptScore W2993763753C54355233 @default.
- W2993763753 hasConceptScore W2993763753C60644358 @default.
- W2993763753 hasConceptScore W2993763753C60987743 @default.
- W2993763753 hasConceptScore W2993763753C70721500 @default.
- W2993763753 hasConceptScore W2993763753C80311884 @default.
- W2993763753 hasConceptScore W2993763753C86803240 @default.
- W2993763753 hasConceptScore W2993763753C89423630 @default.
- W2993763753 hasFunder F4320310921 @default.
- W2993763753 hasFunder F4320320995 @default.
- W2993763753 hasFunder F4320334593 @default.
- W2993763753 hasIssue "1" @default.
- W2993763753 hasLocation W29937637531 @default.
- W2993763753 hasLocation W29937637532 @default.
- W2993763753 hasOpenAccess W2993763753 @default.
- W2993763753 hasPrimaryLocation W29937637531 @default.
- W2993763753 hasRelatedWork W1626468933 @default.
- W2993763753 hasRelatedWork W1967269679 @default.
- W2993763753 hasRelatedWork W1969416270 @default.
- W2993763753 hasRelatedWork W1974681747 @default.
- W2993763753 hasRelatedWork W2036920350 @default.
- W2993763753 hasRelatedWork W2075987129 @default.
- W2993763753 hasRelatedWork W2095908222 @default.
- W2993763753 hasRelatedWork W2142573692 @default.
- W2993763753 hasRelatedWork W2166040922 @default.
- W2993763753 hasRelatedWork W3212263526 @default.
- W2993763753 hasVolume "55" @default.
- W2993763753 isParatext "false" @default.
- W2993763753 isRetracted "false" @default.
- W2993763753 magId "2993763753" @default.
- W2993763753 workType "article" @default.