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• W2994085198 abstract "Background DNA methylation detection is indispensable for the diagnosis and prognosis of various diseases including malignancies. Hence, it is crucial to develop a simple, sensitive, and specific detection strategy. Methods A novel fluorescent biosensor was developed based on a simple dual signal amplification strategy using functional dendritic DNA nanostructure and signal-enriching polystyrene microbeads in combination with ligase detection reaction (LDR). Dendritic DNA self-assembled from Y-DNA and X-DNA through enzyme-free DNA catalysis of a hairpin structure, which was prevented from unwinding at high temperature by adding psoralen. Then dendritic DNA polymer labeled with fluorescent dye Cy5 was ligated with reporter probe into a conjugate. Avidin-labeled polystyrene microbeads were specifically bound to biotin-labeled capture probe, and hybridized with target sequence and dendritic DNA. LDR was triggered by adding Taq ligase. When methylated cytosine existed, the capture probe and reporter probe labeled with fluorescent dye perfectly matched the target sequence, forming a stable duplex to generate a fluorescence signal. However, after bisulfite treatment, unmethylated cytosine was converted into uracil, resulting in a single base mismatch. No fluorescence signal was detected due to the absence of duplex. Results The obtained dendritic DNA polymer had a large volume. This method was time-saving and low-cost. Under the optimal experimental conditions using avidin-labeled polystyrene microbeads, the fluorescence signal was amplified more obviously, and DNA methylation was quantified ultrasensitively and selectively. The detection range of this sensor was 10 −15 to 10 −7 M, and the limit of detection reached as low as 0.4 fM. The constructed biosensor was also successfully used to analyze actual samples. Conclusion This strategy has ultrasensitivity and high specificity for DNA methylation quantification, without requiring complex processes such as PCR and enzymatic digestion, which is thus of great value in tumor diagnosis and biomedical research." @default.
• W2994085198 created "2019-12-13" @default.
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• W2994085198 date "2019-12-01" @default.
• W2994085198 modified "2023-10-14" @default.
• W2994085198 title "A novel fluorescent biosensor based on dendritic DNA nanostructure in combination with ligase reaction for ultrasensitive detection of DNA methylation" @default.
• W2994085198 cites W1506411356 @default.
• W2994085198 cites W1967268303 @default.
• W2994085198 cites W1968271489 @default.
• W2994085198 cites W1970249377 @default.
• W2994085198 cites W1977222616 @default.
• W2994085198 cites W1987557775 @default.
• W2994085198 cites W1988017228 @default.
• W2994085198 cites W1997866877 @default.
• W2994085198 cites W2008820162 @default.
• W2994085198 cites W2010446213 @default.
• W2994085198 cites W2013510852 @default.
• W2994085198 cites W2017502278 @default.
• W2994085198 cites W2036828137 @default.
• W2994085198 cites W2039590701 @default.
• W2994085198 cites W2040507896 @default.
• W2994085198 cites W2046595393 @default.
• W2994085198 cites W2056264420 @default.
• W2994085198 cites W2085245717 @default.
• W2994085198 cites W2089647096 @default.
• W2994085198 cites W2090098058 @default.
• W2994085198 cites W2094471257 @default.
• W2994085198 cites W2109634436 @default.
• W2994085198 cites W2123032740 @default.
• W2994085198 cites W2124748744 @default.
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• W2994085198 cites W2771719355 @default.
• W2994085198 cites W2782702170 @default.
• W2994085198 cites W2785593678 @default.
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• W2994085198 doi "https://doi.org/10.1186/s12951-019-0552-5" @default.
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