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- W2994162541 endingPage "e1008494" @default.
- W2994162541 startingPage "e1008494" @default.
- W2994162541 abstract "Faithful replication and repair of DNA lesions ensure genome maintenance. During replication in eukaryotic cells, DNA is unwound by the CMG helicase complex, which is composed of three major components: the Cdc45 protein, Mcm2-7, and the GINS complex. The CMG in complex with DNA polymerase epsilon (CMG-E) participates in the establishment and progression of the replisome. Impaired functioning of the CMG-E was shown to induce genomic instability and promote the development of various diseases. Therefore, CMG-E components play important roles as caretakers of the genome. In Saccharomyces cerevisiae, the GINS complex is composed of the Psf1, Psf2, Psf3, and Sld5 essential subunits. The Psf1-1 mutant form fails to interact with Psf3, resulting in impaired replisome assembly and chromosome replication. Here, we show increased instability of repeat tracts (mononucleotide, dinucleotide, trinucleotide and longer) in yeast psf1-1 mutants. To identify the mechanisms underlying this effect, we analyzed repeated sequence instability using derivatives of psf1-1 strains lacking genes involved in translesion synthesis, recombination, or mismatch repair. Among these derivatives, deletion of RAD52, RAD51, MMS2, POL32, or PIF1 significantly decreased DNA repeat instability. These results, together with the observed increased amounts of single-stranded DNA regions and Rfa1 foci suggest that recombinational mechanisms make important contributions to repeat tract instability in psf1-1 cells. We propose that defective functioning of the CMG-E complex in psf1-1 cells impairs the progression of DNA replication what increases the contribution of repair mechanisms such as template switch and break-induced replication. These processes require sequence homology search which in case of a repeated DNA tract may result in misalignment leading to its expansion or contraction." @default.
- W2994162541 created "2019-12-13" @default.
- W2994162541 creator A5026896710 @default.
- W2994162541 creator A5042194770 @default.
- W2994162541 creator A5053530523 @default.
- W2994162541 creator A5056513144 @default.
- W2994162541 creator A5063514634 @default.
- W2994162541 creator A5063618315 @default.
- W2994162541 creator A5080901908 @default.
- W2994162541 date "2019-12-09" @default.
- W2994162541 modified "2023-09-25" @default.
- W2994162541 title "Defects in the GINS complex increase the instability of repetitive sequences via a recombination-dependent mechanism" @default.
- W2994162541 cites W1496033005 @default.
- W2994162541 cites W1533555842 @default.
- W2994162541 cites W154063391 @default.
- W2994162541 cites W1611375148 @default.
- W2994162541 cites W170544940 @default.
- W2994162541 cites W1752388152 @default.
- W2994162541 cites W1788309009 @default.
- W2994162541 cites W1827569717 @default.
- W2994162541 cites W1880539669 @default.
- W2994162541 cites W1922072366 @default.
- W2994162541 cites W1945353891 @default.
- W2994162541 cites W1964831588 @default.
- W2994162541 cites W1965493201 @default.
- W2994162541 cites W1965593950 @default.
- W2994162541 cites W1968493165 @default.
- W2994162541 cites W1969737313 @default.
- W2994162541 cites W1970806530 @default.
- W2994162541 cites W1970814720 @default.
- W2994162541 cites W1972024140 @default.
- W2994162541 cites W1972561496 @default.
- W2994162541 cites W1974736523 @default.
- W2994162541 cites W1975001355 @default.
- W2994162541 cites W1977852064 @default.
- W2994162541 cites W1980042463 @default.
- W2994162541 cites W1982716670 @default.
- W2994162541 cites W1984680959 @default.
- W2994162541 cites W1984829206 @default.
- W2994162541 cites W1984865535 @default.
- W2994162541 cites W1985491895 @default.
- W2994162541 cites W1988780940 @default.
- W2994162541 cites W1990214856 @default.
- W2994162541 cites W1994775292 @default.
- W2994162541 cites W1997854301 @default.
- W2994162541 cites W1997859335 @default.
- W2994162541 cites W1998277140 @default.
- W2994162541 cites W1998758644 @default.
- W2994162541 cites W2002093858 @default.
- W2994162541 cites W2003023768 @default.
- W2994162541 cites W2003696878 @default.
- W2994162541 cites W2004102090 @default.
- W2994162541 cites W2006764478 @default.
- W2994162541 cites W2007888538 @default.
- W2994162541 cites W2008583061 @default.
- W2994162541 cites W2009999911 @default.
- W2994162541 cites W2011367808 @default.
- W2994162541 cites W2015181216 @default.
- W2994162541 cites W2016357039 @default.
- W2994162541 cites W2017431335 @default.
- W2994162541 cites W2018713333 @default.
- W2994162541 cites W2019932549 @default.
- W2994162541 cites W2024429438 @default.
- W2994162541 cites W2025327704 @default.
- W2994162541 cites W2028682744 @default.
- W2994162541 cites W2029369774 @default.
- W2994162541 cites W2034090934 @default.
- W2994162541 cites W2034216542 @default.
- W2994162541 cites W2038220637 @default.
- W2994162541 cites W2038909539 @default.
- W2994162541 cites W2042160009 @default.
- W2994162541 cites W2042854528 @default.
- W2994162541 cites W2048975947 @default.
- W2994162541 cites W2053903416 @default.
- W2994162541 cites W2057218806 @default.
- W2994162541 cites W2058598374 @default.
- W2994162541 cites W2059788298 @default.
- W2994162541 cites W2063847889 @default.
- W2994162541 cites W2065816205 @default.
- W2994162541 cites W2066618008 @default.
- W2994162541 cites W2069157381 @default.
- W2994162541 cites W2072295634 @default.
- W2994162541 cites W2072840521 @default.
- W2994162541 cites W2073568663 @default.
- W2994162541 cites W2073969486 @default.
- W2994162541 cites W2074451382 @default.
- W2994162541 cites W2075709117 @default.
- W2994162541 cites W2077570881 @default.
- W2994162541 cites W2082507962 @default.
- W2994162541 cites W2083344047 @default.
- W2994162541 cites W2097095628 @default.
- W2994162541 cites W2099540110 @default.
- W2994162541 cites W2105329904 @default.
- W2994162541 cites W2106837356 @default.
- W2994162541 cites W2107333316 @default.
- W2994162541 cites W2108485315 @default.
- W2994162541 cites W2108931268 @default.
- W2994162541 cites W2111434929 @default.