FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2994424043> ?p ?o ?g. }

• W2994424043 abstract "Abstract Bioproduction of chemical compounds is of great interest for modern industries, as it reduces their production costs and ecological impact. With the use of synthetic biology, metabolic engineering and enzyme engineering tools, the yield of production can be improved to reach mass production and cost-effectiveness expectations. In this study, we explore the bioproduction of D-psicose, also known as D-allulose, a rare non-toxic sugar and a sweetener present in nature in low amounts. D-psicose has interesting properties and seemingly the ability to fight against obesity and type 2 diabetes. We developed a biosensor-based enzyme screening approach as a tool for enzyme selection that we benchmarked with the Clostridium cellulolyticum D-psicose 3-epimerase for the production of D-psicose from D-fructose. For this purpose, we constructed and characterized seven psicose responsive biosensors based on previously uncharacterized transcription factors and either their predicted promoters or an engineered promoter. In order to standardize our system, we created the Universal Biosensor Chassis, a construct with a highly modular architecture that allows rapid engineering of any transcription factor-based biosensor. Among the seven biosensors, we chose the one displaying the most linear behavior and the highest increase in fluorescence fold change. Next, we generated a library of D-psicose 3-epimerase mutants by error-prone PCR and screened it using the biosensor to select gain of function enzyme mutants, thus demonstrating the framework’s efficiency." @default.
• W2994424043 created "2019-12-13" @default.
• W2994424043 creator A5006931152 @default.
• W2994424043 creator A5028709322 @default.
• W2994424043 creator A5029640814 @default.
• W2994424043 creator A5033348600 @default.
• W2994424043 creator A5033517557 @default.
• W2994424043 creator A5080916918 @default.
• W2994424043 creator A5084508175 @default.
• W2994424043 date "2019-01-01" @default.
• W2994424043 modified "2023-10-16" @default.
• W2994424043 title "Biosensor-based enzyme engineering approach applied to psicose biosynthesis" @default.
• W2994424043 cites W1151175600 @default.
• W2994424043 cites W1606435569 @default.
• W2994424043 cites W1789943808 @default.
• W2994424043 cites W1902653299 @default.
• W2994424043 cites W1948571680 @default.
• W2994424043 cites W1969996020 @default.
• W2994424043 cites W1994286229 @default.
• W2994424043 cites W2008470319 @default.
• W2994424043 cites W2014133014 @default.
• W2994424043 cites W2016052066 @default.
• W2994424043 cites W2016667239 @default.
• W2994424043 cites W2035425324 @default.
• W2994424043 cites W2043462765 @default.
• W2994424043 cites W2066529216 @default.
• W2994424043 cites W2081799587 @default.
• W2994424043 cites W2107405889 @default.
• W2994424043 cites W2115734610 @default.
• W2994424043 cites W2129660964 @default.
• W2994424043 cites W2130708697 @default.
• W2994424043 cites W2130879035 @default.
• W2994424043 cites W2160253662 @default.
• W2994424043 cites W2161273461 @default.
• W2994424043 cites W2161782059 @default.
• W2994424043 cites W2163679306 @default.
• W2994424043 cites W2258180218 @default.
• W2994424043 cites W2260617737 @default.
• W2994424043 cites W2276368784 @default.
• W2994424043 cites W2315357306 @default.
• W2994424043 cites W2329160228 @default.
• W2994424043 cites W2335566724 @default.
• W2994424043 cites W2341006608 @default.
• W2994424043 cites W2476717220 @default.
• W2994424043 cites W2501356178 @default.
• W2994424043 cites W2616983714 @default.
• W2994424043 cites W2724107504 @default.
• W2994424043 cites W2748778291 @default.
• W2994424043 cites W2766823458 @default.
• W2994424043 cites W2770836247 @default.
• W2994424043 cites W2779501236 @default.
• W2994424043 cites W2792271439 @default.
• W2994424043 cites W2792415449 @default.
• W2994424043 cites W2800383367 @default.
• W2994424043 cites W2867072378 @default.
• W2994424043 cites W2897029973 @default.
• W2994424043 cites W2904752569 @default.
• W2994424043 cites W2963737470 @default.
• W2994424043 cites W2970341269 @default.
• W2994424043 cites W744818514 @default.
• W2994424043 cites W847394227 @default.
• W2994424043 doi "https://doi.org/10.1093/synbio/ysz028" @default.
• W2994424043 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7445875" @default.
• W2994424043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32995548" @default.
• W2994424043 hasPublicationYear "2019" @default.
• W2994424043 type Work @default.
• W2994424043 sameAs 2994424043 @default.
• W2994424043 citedByCount "13" @default.
• W2994424043 countsByYear W29944240432021 @default.
• W2994424043 countsByYear W29944240432022 @default.
• W2994424043 countsByYear W29944240432023 @default.
• W2994424043 crossrefType "journal-article" @default.
• W2994424043 hasAuthorship W2994424043A5006931152 @default.
• W2994424043 hasAuthorship W2994424043A5028709322 @default.
• W2994424043 hasAuthorship W2994424043A5029640814 @default.
• W2994424043 hasAuthorship W2994424043A5033348600 @default.
• W2994424043 hasAuthorship W2994424043A5033517557 @default.
• W2994424043 hasAuthorship W2994424043A5080916918 @default.
• W2994424043 hasAuthorship W2994424043A5084508175 @default.
• W2994424043 hasBestOaLocation W29944240431 @default.
• W2994424043 hasConcept C104317684 @default.
• W2994424043 hasConcept C143065580 @default.
• W2994424043 hasConcept C147816474 @default.
• W2994424043 hasConcept C160756335 @default.
• W2994424043 hasConcept C181199279 @default.
• W2994424043 hasConcept C185592680 @default.
• W2994424043 hasConcept C191908910 @default.
• W2994424043 hasConcept C2779227428 @default.
• W2994424043 hasConcept C41008148 @default.
• W2994424043 hasConcept C55493867 @default.
• W2994424043 hasConcept C6350086 @default.
• W2994424043 hasConcept C70721500 @default.
• W2994424043 hasConcept C86803240 @default.
• W2994424043 hasConcept C9418097 @default.
• W2994424043 hasConceptScore W2994424043C104317684 @default.
• W2994424043 hasConceptScore W2994424043C143065580 @default.
• W2994424043 hasConceptScore W2994424043C147816474 @default.
• W2994424043 hasConceptScore W2994424043C160756335 @default.
• W2994424043 hasConceptScore W2994424043C181199279 @default.
• W2994424043 hasConceptScore W2994424043C185592680 @default.

• next