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- W2994493705 abstract "[Objective] The aim was to establish an event-specific qualitative PCR method for transgenic soybean MON89788.[Method] Firstly,the 3′-junction sequence between host plant DNA and integrated DNA of transgenic MON89788 soybean was isolated using thermal asymmetric interlaced-PCR (TAIL-PCR),and the specific PCR primers were designed based on the 3′-junction sequence.Secondly,the specificity and sensitivity of the qualitative PCR detection methods employing these primers were tested.[Result] 1 142-bp 3′-junction sequence was obtained.According to the sequence,event-specific qualitative PCR method was established,amplifying a 170-bp product specifically from MON89788 event,and the limit of detection was 0.05%,approximately 40 initial template copies.[Conclusion] The method was highly specific,sensitive,and suitable for detection of MON89788 event." @default.
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- W2994493705 date "2010-01-01" @default.
- W2994493705 modified "2023-10-03" @default.
- W2994493705 title "Establishment of Event-specific Qualitative PCR Method for Genetically Modified Soybean MON 89788" @default.
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