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- W2994829044 abstract "Establishing how water, or the absence of water, affects the structure, dynamics, and function of proteins in contact with inorganic surfaces is critical to developing successful protein immobilization strategies. In the present article, the quantity of water hydrating a monolayer of helical peptides covalently attached to self-assembled monolayers (SAMs) of alkyl thiols on Au was measured using neutron reflectometry (NR). The peptide sequence was composed of repeating LLKK units in which the leucines were aligned to face the SAM. When immersed in water, NR measured 2.7 ± 0.9 water molecules per thiol in the SAM layer and between 75 ± 13 and 111 ± 13 waters around each peptide. The quantity of water in the SAM was nearly twice that measured prior to peptide functionalization, suggesting that the peptide disrupted the structure of the SAM. To identify the location of water molecules around the peptide, we compared our NR data to previously published molecular dynamics simulations of the same peptide on a hydrophobic SAM in water, revealing that 49 ± 5 of 95 ± 8 total nearby water molecules were directly hydrogen-bound to the peptide. Finally, we show that immersing the peptide in water compressed its structure into the SAM surface. Together, these results demonstrate that there is sufficient water to fully hydrate a surface-bound peptide even at hydrophobic interfaces. Given the critical role that water plays in biomolecular structure and function, these results are expected to be informative for a broad array of applications involving proteins at the bio/abio interface." @default.
- W2994829044 created "2019-12-26" @default.
- W2994829044 creator A5034034924 @default.
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- W2994829044 creator A5063363672 @default.
- W2994829044 creator A5082332911 @default.
- W2994829044 date "2019-12-17" @default.
- W2994829044 modified "2023-10-16" @default.
- W2994829044 title "Quantifying the Extent of Hydration of a Surface-Bound Peptide Using Neutron Reflectometry" @default.
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- W2994829044 cites W1624860839 @default.
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- W2994829044 cites W1973574290 @default.
- W2994829044 cites W1974730206 @default.
- W2994829044 cites W1978633332 @default.
- W2994829044 cites W1981021420 @default.
- W2994829044 cites W1983945471 @default.
- W2994829044 cites W1989515624 @default.
- W2994829044 cites W2018437827 @default.
- W2994829044 cites W2021630428 @default.
- W2994829044 cites W2023022008 @default.
- W2994829044 cites W2027670638 @default.
- W2994829044 cites W2029667189 @default.
- W2994829044 cites W2031342733 @default.
- W2994829044 cites W2037134261 @default.
- W2994829044 cites W2038668766 @default.
- W2994829044 cites W2042848406 @default.
- W2994829044 cites W2044252957 @default.
- W2994829044 cites W2047154723 @default.
- W2994829044 cites W2047981591 @default.
- W2994829044 cites W2051232092 @default.
- W2994829044 cites W2057347415 @default.
- W2994829044 cites W2057742133 @default.
- W2994829044 cites W2057876883 @default.
- W2994829044 cites W2059754495 @default.
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- W2994829044 cites W2065571647 @default.
- W2994829044 cites W2067910314 @default.
- W2994829044 cites W2078224817 @default.
- W2994829044 cites W2082063506 @default.
- W2994829044 cites W2091176202 @default.
- W2994829044 cites W2092758215 @default.
- W2994829044 cites W2107239101 @default.
- W2994829044 cites W2107573334 @default.
- W2994829044 cites W2122455742 @default.
- W2994829044 cites W2123768693 @default.
- W2994829044 cites W2126971049 @default.
- W2994829044 cites W2134819724 @default.
- W2994829044 cites W2141723860 @default.
- W2994829044 cites W2145665280 @default.
- W2994829044 cites W2158558808 @default.
- W2994829044 cites W2160544821 @default.
- W2994829044 cites W2164269590 @default.
- W2994829044 cites W2166806803 @default.
- W2994829044 cites W2171268876 @default.
- W2994829044 cites W2172945004 @default.
- W2994829044 cites W2313959357 @default.
- W2994829044 cites W2327808910 @default.
- W2994829044 cites W2332928107 @default.
- W2994829044 cites W2333557439 @default.
- W2994829044 cites W2333573414 @default.
- W2994829044 cites W2333779976 @default.
- W2994829044 cites W2340639121 @default.
- W2994829044 cites W2395521783 @default.
- W2994829044 cites W2511028525 @default.
- W2994829044 cites W2529012845 @default.
- W2994829044 cites W2550436958 @default.
- W2994829044 cites W2596060510 @default.
- W2994829044 cites W2607313338 @default.
- W2994829044 cites W2782674897 @default.
- W2994829044 cites W2788246252 @default.
- W2994829044 cites W2809466999 @default.
- W2994829044 cites W2886510904 @default.
- W2994829044 cites W2896882920 @default.
- W2994829044 cites W2909618425 @default.
- W2994829044 cites W2917398733 @default.
- W2994829044 cites W2935156750 @default.
- W2994829044 cites W2942532320 @default.
- W2994829044 cites W2954074974 @default.
- W2994829044 cites W2962696297 @default.
- W2994829044 cites W848448822 @default.
- W2994829044 cites W97269328 @default.
- W2994829044 cites W2332513905 @default.
- W2994829044 doi "https://doi.org/10.1021/acs.langmuir.9b02559" @default.
- W2994829044 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31846580" @default.
- W2994829044 hasPublicationYear "2019" @default.
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