Matches in SemOpenAlex for { <https://semopenalex.org/work/W2994839684> ?p ?o ?g. }
- W2994839684 endingPage "131" @default.
- W2994839684 startingPage "125" @default.
- W2994839684 abstract "Here we present a novel protocol for the construction of saturation single-site—and massive multisite—mutant libraries of a bacteriophage. We segmented the ΦX174 genome into 14 nontoxic and nonreplicative fragments compatible with Golden Gate assembly. We next used nicking mutagenesis with oligonucleotides prepared from unamplified oligo pools with individual segments as templates to prepare near-comprehensive single-site mutagenesis libraries of genes encoding the F capsid protein (421 amino acids scanned) and G spike protein (172 amino acids scanned). Libraries possessed greater than 99% of all 11 860 programmed mutations. Golden Gate cloning was then used to assemble the complete ΦX174 mutant genome and generate libraries of infective viruses. This protocol will enable reverse genetics experiments for studying viral evolution and, with some modifications, can be applied for engineering therapeutically relevant bacteriophages with larger genomes." @default.
- W2994839684 created "2019-12-26" @default.
- W2994839684 creator A5014658449 @default.
- W2994839684 creator A5016737202 @default.
- W2994839684 creator A5022039008 @default.
- W2994839684 creator A5047900245 @default.
- W2994839684 creator A5054775452 @default.
- W2994839684 creator A5070409869 @default.
- W2994839684 creator A5070975430 @default.
- W2994839684 creator A5084408934 @default.
- W2994839684 date "2019-12-11" @default.
- W2994839684 modified "2023-10-02" @default.
- W2994839684 title "Saturation Mutagenesis Genome Engineering of Infective ΦX174 Bacteriophage <i>via</i> Unamplified Oligo Pools and Golden Gate Assembly" @default.
- W2994839684 cites W1525355465 @default.
- W2994839684 cites W1614600542 @default.
- W2994839684 cites W1969094530 @default.
- W2994839684 cites W1974849183 @default.
- W2994839684 cites W1995039292 @default.
- W2994839684 cites W1997478924 @default.
- W2994839684 cites W2003393395 @default.
- W2994839684 cites W2006085890 @default.
- W2994839684 cites W2014470952 @default.
- W2994839684 cites W2026916707 @default.
- W2994839684 cites W2029583307 @default.
- W2994839684 cites W2034786222 @default.
- W2994839684 cites W2039761134 @default.
- W2994839684 cites W2045219947 @default.
- W2994839684 cites W2060588922 @default.
- W2994839684 cites W2092247774 @default.
- W2994839684 cites W2096666231 @default.
- W2994839684 cites W2104921450 @default.
- W2994839684 cites W2108666079 @default.
- W2994839684 cites W2115486909 @default.
- W2994839684 cites W2123123794 @default.
- W2994839684 cites W2136696257 @default.
- W2994839684 cites W2144893575 @default.
- W2994839684 cites W2167653189 @default.
- W2994839684 cites W2171197491 @default.
- W2994839684 cites W2337003117 @default.
- W2994839684 cites W2409985107 @default.
- W2994839684 cites W2530338180 @default.
- W2994839684 cites W2555700585 @default.
- W2994839684 cites W2603578729 @default.
- W2994839684 cites W2767892805 @default.
- W2994839684 cites W2796415147 @default.
- W2994839684 cites W2796780818 @default.
- W2994839684 cites W2797284528 @default.
- W2994839684 cites W2802851075 @default.
- W2994839684 cites W2906596870 @default.
- W2994839684 cites W2907505942 @default.
- W2994839684 cites W2943878465 @default.
- W2994839684 cites W2950672524 @default.
- W2994839684 cites W2958084955 @default.
- W2994839684 cites W2967635929 @default.
- W2994839684 cites W2977502951 @default.
- W2994839684 cites W3100998278 @default.
- W2994839684 cites W4239871258 @default.
- W2994839684 cites W4250114104 @default.
- W2994839684 doi "https://doi.org/10.1021/acssynbio.9b00411" @default.
- W2994839684 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7055157" @default.
- W2994839684 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31825605" @default.
- W2994839684 hasPublicationYear "2019" @default.
- W2994839684 type Work @default.
- W2994839684 sameAs 2994839684 @default.
- W2994839684 citedByCount "8" @default.
- W2994839684 countsByYear W29948396842020 @default.
- W2994839684 countsByYear W29948396842021 @default.
- W2994839684 countsByYear W29948396842023 @default.
- W2994839684 crossrefType "journal-article" @default.
- W2994839684 hasAuthorship W2994839684A5014658449 @default.
- W2994839684 hasAuthorship W2994839684A5016737202 @default.
- W2994839684 hasAuthorship W2994839684A5022039008 @default.
- W2994839684 hasAuthorship W2994839684A5047900245 @default.
- W2994839684 hasAuthorship W2994839684A5054775452 @default.
- W2994839684 hasAuthorship W2994839684A5070409869 @default.
- W2994839684 hasAuthorship W2994839684A5070975430 @default.
- W2994839684 hasAuthorship W2994839684A5084408934 @default.
- W2994839684 hasBestOaLocation W29948396842 @default.
- W2994839684 hasConcept C104317684 @default.
- W2994839684 hasConcept C109129352 @default.
- W2994839684 hasConcept C134486438 @default.
- W2994839684 hasConcept C13575015 @default.
- W2994839684 hasConcept C141231307 @default.
- W2994839684 hasConcept C143065580 @default.
- W2994839684 hasConcept C144501496 @default.
- W2994839684 hasConcept C145290725 @default.
- W2994839684 hasConcept C147816474 @default.
- W2994839684 hasConcept C16318435 @default.
- W2994839684 hasConcept C167625842 @default.
- W2994839684 hasConcept C181199279 @default.
- W2994839684 hasConcept C191908910 @default.
- W2994839684 hasConcept C202878990 @default.
- W2994839684 hasConcept C25365240 @default.
- W2994839684 hasConcept C2776441376 @default.
- W2994839684 hasConcept C515207424 @default.
- W2994839684 hasConcept C54355233 @default.
- W2994839684 hasConcept C547475151 @default.
- W2994839684 hasConcept C552990157 @default.