Matches in SemOpenAlex for { <https://semopenalex.org/work/W2994876269> ?p ?o ?g. }
- W2994876269 endingPage "e201900542" @default.
- W2994876269 startingPage "e201900542" @default.
- W2994876269 abstract "Host interferon-induced transmembrane proteins (IFITMs) are broad-spectrum antiviral restriction factors. Of these, IFITM3 potently inhibits viruses that enter cells through acidic endosomes, many of which are zoonotic and emerging viruses with bats (order Chiroptera) as their natural hosts. We previously demonstrated that microbat IFITM3 is antiviral. Here, we show that bat IFITMs are characterized by strong adaptive evolution and identify a highly variable and functionally important site—codon 70—within the conserved CD225 domain of IFITMs. Mutation of this residue in microbat IFITM3 impairs restriction of representatives of four different virus families that enter cells via endosomes. This mutant shows altered subcellular localization and reduced S-palmitoylation, a phenotype copied by mutation of conserved cysteine residues in microbat IFITM3. Furthermore, we show that microbat IFITM3 is S-palmitoylated on cysteine residues C71, C72, and C105, mutation of each cysteine individually impairs virus restriction, and a triple C71A-C72A-C105A mutant loses all restriction activity, concomitant with subcellular re-localization of microbat IFITM3 to Golgi-associated sites. Thus, we propose that S-palmitoylation is critical for Chiropteran IFITM3 function and identify a key molecular determinant of IFITM3 S-palmitoylation." @default.
- W2994876269 created "2019-12-26" @default.
- W2994876269 creator A5001533319 @default.
- W2994876269 creator A5013838021 @default.
- W2994876269 creator A5016529322 @default.
- W2994876269 creator A5021686242 @default.
- W2994876269 creator A5038049864 @default.
- W2994876269 creator A5065105540 @default.
- W2994876269 creator A5067304304 @default.
- W2994876269 creator A5068652062 @default.
- W2994876269 creator A5079491369 @default.
- W2994876269 creator A5084170505 @default.
- W2994876269 creator A5085736091 @default.
- W2994876269 date "2019-12-11" @default.
- W2994876269 modified "2023-10-12" @default.
- W2994876269 title "Bat IFITM3 restriction depends on S-palmitoylation and a polymorphic site within the CD225 domain" @default.
- W2994876269 cites W1553217569 @default.
- W2994876269 cites W1845946429 @default.
- W2994876269 cites W1869567261 @default.
- W2994876269 cites W1963957860 @default.
- W2994876269 cites W1965606791 @default.
- W2994876269 cites W1979358876 @default.
- W2994876269 cites W1982698840 @default.
- W2994876269 cites W1983304589 @default.
- W2994876269 cites W1990581210 @default.
- W2994876269 cites W1995684348 @default.
- W2994876269 cites W1998351815 @default.
- W2994876269 cites W1998917031 @default.
- W2994876269 cites W2001353863 @default.
- W2994876269 cites W2007539090 @default.
- W2994876269 cites W2014098988 @default.
- W2994876269 cites W2014758253 @default.
- W2994876269 cites W2015985276 @default.
- W2994876269 cites W2017388217 @default.
- W2994876269 cites W2018925580 @default.
- W2994876269 cites W2024284897 @default.
- W2994876269 cites W2028170369 @default.
- W2994876269 cites W2029134836 @default.
- W2994876269 cites W2032304369 @default.
- W2994876269 cites W2034324831 @default.
- W2994876269 cites W2042594196 @default.
- W2994876269 cites W2048816591 @default.
- W2994876269 cites W2052775071 @default.
- W2994876269 cites W2055677723 @default.
- W2994876269 cites W2062491810 @default.
- W2994876269 cites W2068946828 @default.
- W2994876269 cites W2069831575 @default.
- W2994876269 cites W2073944948 @default.
- W2994876269 cites W2074303266 @default.
- W2994876269 cites W2076617993 @default.
- W2994876269 cites W2078063242 @default.
- W2994876269 cites W2078896168 @default.
- W2994876269 cites W2082703775 @default.
- W2994876269 cites W2087482145 @default.
- W2994876269 cites W2089422286 @default.
- W2994876269 cites W2094323269 @default.
- W2994876269 cites W2103546861 @default.
- W2994876269 cites W2108512869 @default.
- W2994876269 cites W2111937747 @default.
- W2994876269 cites W2114421191 @default.
- W2994876269 cites W2128880918 @default.
- W2994876269 cites W2129285015 @default.
- W2994876269 cites W2135264287 @default.
- W2994876269 cites W2143314736 @default.
- W2994876269 cites W2147710356 @default.
- W2994876269 cites W2159375800 @default.
- W2994876269 cites W2159957100 @default.
- W2994876269 cites W2164084422 @default.
- W2994876269 cites W2166964088 @default.
- W2994876269 cites W2287025468 @default.
- W2994876269 cites W2293880967 @default.
- W2994876269 cites W2319428081 @default.
- W2994876269 cites W2320452086 @default.
- W2994876269 cites W2399769146 @default.
- W2994876269 cites W2412098581 @default.
- W2994876269 cites W2417724913 @default.
- W2994876269 cites W2471419727 @default.
- W2994876269 cites W2487259150 @default.
- W2994876269 cites W2517466029 @default.
- W2994876269 cites W2626734936 @default.
- W2994876269 cites W2700382303 @default.
- W2994876269 cites W2745504864 @default.
- W2994876269 cites W2754064883 @default.
- W2994876269 cites W2765258268 @default.
- W2994876269 cites W2768463336 @default.
- W2994876269 cites W2770032478 @default.
- W2994876269 cites W2778151539 @default.
- W2994876269 cites W2779096414 @default.
- W2994876269 cites W2783458468 @default.
- W2994876269 cites W2787348393 @default.
- W2994876269 cites W2788713245 @default.
- W2994876269 cites W2792034759 @default.
- W2994876269 cites W2799839529 @default.
- W2994876269 cites W2885775156 @default.
- W2994876269 cites W2890126170 @default.
- W2994876269 cites W2906507079 @default.
- W2994876269 cites W2906753872 @default.
- W2994876269 cites W2910275920 @default.