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- W2994920782 abstract "Actin dynamics generate forces to deform the membrane and overcome the cell’s high turgor pressure during clathrin-mediated endocytosis (CME) in yeast, but precise molecular details are still unresolved. Our previous models predicted that actin filaments of the endocytic meshwork continually polymerize and disassemble, turning over multiple times during an endocytic event, similar to other actin systems. We applied single-molecule speckle tracking in live fission yeast to directly measure molecular turnover within CME sites for the first time. In contrast with the overall ~20 s lifetimes of actin and actin-associated proteins in endocytic patches, we detected single-molecule residence times around 1 to 2 s, and similarly high turnover rates of membrane-associated proteins in CME. Furthermore, we find heterogeneous behaviors in many proteins’ motions. These results indicate that endocytic proteins turn over up to five times during the formation of an endocytic vesicle, and suggest revising quantitative models of force production." @default.
- W2994920782 created "2019-12-26" @default.
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- W2994920782 creator A5061088743 @default.
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- W2994920782 date "2019-12-19" @default.
- W2994920782 modified "2023-10-16" @default.
- W2994920782 title "Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis" @default.
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- W2994920782 doi "https://doi.org/10.7554/elife.52355" @default.
- W2994920782 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6977972" @default.
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