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• W2995062022 abstract "Advances in biosensor engineering have enabled the design of programmable molecular systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer and field-test a biosensor for fluoride, a major groundwater contaminant of global concern. The sensor consists of a cell-free system containing a DNA template that encodes a fluoride-responsive riboswitch regulating genes that produce a fluorescent or colorimetric output. Individual reactions can be lyophilized for long-term storage and detect fluoride at levels above 2 ppm, the Environmental Protection Agency’s most stringent regulatory standard, in both laboratory and field conditions. Through onsite detection of fluoride in a real-world water source, this work provides a critical proof-of-principle for the future engineering of riboswitches and other biosensors to address challenges for global health and the environment." @default.
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• W2995062022 date "2019-12-12" @default.
• W2995062022 modified "2023-10-17" @default.
• W2995062022 title "Point-of-Use Detection of Environmental Fluoride <i>via</i> a Cell-Free Riboswitch-Based Biosensor" @default.
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• W2995062022 doi "https://doi.org/10.1021/acssynbio.9b00347" @default.
• W2995062022 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7412506" @default.
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