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• W2995122020 abstract "Abstract Modeling age-related macular degeneration (AMD) is challenging, because it is a multifactorial disease. To focus on interactions between the retinal pigment epithelium (RPE) and Bruch's membrane, we generated RPE from AMD patients and used an altered extracellular matrix (ECM) that models aged Bruch's membrane. Induced pluripotent stem cells (iPSCs) were generated from fibroblasts isolated from AMD patients or age-matched (normal) controls. RPE derived from iPSCs were analyzed by morphology, marker expression, transepithelial electrical resistance (TER), and phagocytosis of rod photoreceptor outer segments. Cell attachment and viability was tested on nitrite-modified ECM, a typical modification of aged Bruch's membrane. DNA microarrays with hierarchical clustering and analysis of mitochondrial function were used to elucidate possible mechanisms for the observed phenotypes. Differentiated RPE displayed cell-specific morphology and markers. The TER and phagocytic capacity were similar among iPSC-derived RPE cultures. However, distinct clusters were found for the transcriptomes of AMD and control iPSC-derived RPE. AMD-derived iPSC-RPE downregulated genes responsible for metabolic-related pathways and cell attachment. AMD-derived iPSC-RPE exhibited reduced mitochondrial respiration and ability to attach and survive on nitrite-modified ECM. Cells that did attach induced the expression of complement genes. Despite reprogramming, iPSC derived from AMD patients yielded RPE with a transcriptome that is distinct from that of age-matched controls. When challenged with an AMD-like modification of Bruch's membrane, AMD-derived iPSC-RPE activated the complement immune system. Significance statement Age-related macular degeneration (AMD) is a multifactorial disease of primates, which confounds efforts to model the disease in laboratory animals or in culture. The present culture model mimics aspects of the disease, as demonstrated by using stem cells derived from patients. The disease involves interactions between the retinal pigment epithelium, a layer of cells necessary for the function of photoreceptors, and the extracellular matrix upon which the epithelium sits. The differences observed between epithelia derived from normal and AMD patients were exacerbated when cells were cultured on a mimetic for diseased matrix. This model provides a robust platform for developing therapeutics." @default.
• W2995122020 created "2019-12-26" @default.
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• W2995122020 date "2019-12-16" @default.
• W2995122020 modified "2023-10-02" @default.
• W2995122020 title "Stem cell-derived retinal pigment epithelium from patients with age-related macular degeneration exhibit reduced metabolism and matrix interactions" @default.
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• W2995122020 doi "https://doi.org/10.1002/sctm.19-0321" @default.
• W2995122020 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7031648" @default.
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