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• W2995415982 abstract "The excited state lifetime of a fluorophore together with its fluorescence emission spectrum provide information that can yield valuable insights into the nature of a fluorophore and its microenvironment. However, it is difficult to obtain both channels of information in a conventional scheme as detectors are typically configured either for spectral or lifetime detection. We present a fiber-based method to obtain spectral information from a multiphoton fluorescence lifetime imaging (FLIM) system. This is made possible using the time delay introduced in the fluorescence emission path by a dispersive optical fiber coupled to a detector operating in time-correlated single-photon counting mode. This add-on spectral implementation requires only a few simple modifications to any existing FLIM system and is considerably more cost-efficient compared to currently available spectral detectors." @default.
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• W2995415982 date "2019-12-12" @default.
• W2995415982 modified "2023-10-06" @default.
• W2995415982 title "Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems" @default.
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• W2995415982 doi "https://doi.org/10.1117/1.jbo.25.1.014506" @default.
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