Matches in SemOpenAlex for { <https://semopenalex.org/work/W2995458275> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2995458275 abstract "Myelin is a multilamellar sheath that wraps the axons in the Central Nervous System (CNS) and in the Peripheral Nervous System (PNS). It is required for normal development, axonal health and to facilitate impulse conduction. MBP, one of the major myelin proteins, is synthesised by both oligodendrocytes (OL) in CNS and Schwann cells (SC) in PNS. Its synthesis is regulated primarily at the transcriptional level. MBP transcription is activated as a part of a program of coordinate myelin gene expression during development, overlapping but specific in OL as compared to SC. The nuclear proteins that bind to the MBP promoter are largely unidentified. In vitro and in vivo studies show that activation of the MBP proximal promoter requires different DNA binding sites and proteins in OL as compared to SC. Recently, transgenic mice studies identified a SC enhancer (SCE) located 9.0Kb upstream of the transcriptional start site of MBP. In this work, we compared MBP transcription mechanisms in OL and SC. Transient transfection analyses, in primary cultures of OL and SC, confirm the importance of SCE, and suggest that SCE is an activator in both cell types, but it is an enhancer only in SC. Biochemical analyses show that there are regions differentially protected by SC and OL nuclear extracts that can account for differential function. Analysis of internal deletions and transversion mutations of SCE suggests that multiple cis-acting elements cooperate to produce activation. Analyses of stably transfected SC show that chromatin upregulates SCE activation at least three fold. Co-culture of these stably transfected SC and dorsal root ganglia neurons reveal that axons markedly activate SCE, by at least two orders of magnitude. These results describe a model in which to analyse SCE biochemistry and function and identify trans-acting signals from axons that regulate MBP transcription in SC." @default.
- W2995458275 created "2019-12-26" @default.
- W2995458275 creator A5062270935 @default.
- W2995458275 date "2002-05-17" @default.
- W2995458275 modified "2023-09-27" @default.
- W2995458275 title "Distal transcriptional activators of the myelin basic protein gene in oligodendrocytes and Schwann cells" @default.
- W2995458275 doi "https://doi.org/10.21954/ou.ro.0000fbc6" @default.
- W2995458275 hasPublicationYear "2002" @default.
- W2995458275 type Work @default.
- W2995458275 sameAs 2995458275 @default.
- W2995458275 citedByCount "0" @default.
- W2995458275 crossrefType "dissertation" @default.
- W2995458275 hasAuthorship W2995458275A5062270935 @default.
- W2995458275 hasConcept C104317684 @default.
- W2995458275 hasConcept C111936080 @default.
- W2995458275 hasConcept C153911025 @default.
- W2995458275 hasConcept C169760540 @default.
- W2995458275 hasConcept C185592680 @default.
- W2995458275 hasConcept C27153228 @default.
- W2995458275 hasConcept C2776985911 @default.
- W2995458275 hasConcept C2778609137 @default.
- W2995458275 hasConcept C2780876595 @default.
- W2995458275 hasConcept C529278444 @default.
- W2995458275 hasConcept C54009773 @default.
- W2995458275 hasConcept C54355233 @default.
- W2995458275 hasConcept C86339819 @default.
- W2995458275 hasConcept C86803240 @default.
- W2995458275 hasConcept C88045685 @default.
- W2995458275 hasConcept C95444343 @default.
- W2995458275 hasConceptScore W2995458275C104317684 @default.
- W2995458275 hasConceptScore W2995458275C111936080 @default.
- W2995458275 hasConceptScore W2995458275C153911025 @default.
- W2995458275 hasConceptScore W2995458275C169760540 @default.
- W2995458275 hasConceptScore W2995458275C185592680 @default.
- W2995458275 hasConceptScore W2995458275C27153228 @default.
- W2995458275 hasConceptScore W2995458275C2776985911 @default.
- W2995458275 hasConceptScore W2995458275C2778609137 @default.
- W2995458275 hasConceptScore W2995458275C2780876595 @default.
- W2995458275 hasConceptScore W2995458275C529278444 @default.
- W2995458275 hasConceptScore W2995458275C54009773 @default.
- W2995458275 hasConceptScore W2995458275C54355233 @default.
- W2995458275 hasConceptScore W2995458275C86339819 @default.
- W2995458275 hasConceptScore W2995458275C86803240 @default.
- W2995458275 hasConceptScore W2995458275C88045685 @default.
- W2995458275 hasConceptScore W2995458275C95444343 @default.
- W2995458275 hasLocation W29954582751 @default.
- W2995458275 hasOpenAccess W2995458275 @default.
- W2995458275 hasPrimaryLocation W29954582751 @default.
- W2995458275 hasRelatedWork W1867441323 @default.
- W2995458275 hasRelatedWork W1887043131 @default.
- W2995458275 hasRelatedWork W1906187142 @default.
- W2995458275 hasRelatedWork W2007854851 @default.
- W2995458275 hasRelatedWork W2013602440 @default.
- W2995458275 hasRelatedWork W2018041050 @default.
- W2995458275 hasRelatedWork W2031618644 @default.
- W2995458275 hasRelatedWork W2032014984 @default.
- W2995458275 hasRelatedWork W2032382958 @default.
- W2995458275 hasRelatedWork W2047400201 @default.
- W2995458275 hasRelatedWork W2050072301 @default.
- W2995458275 hasRelatedWork W2073133756 @default.
- W2995458275 hasRelatedWork W2083761511 @default.
- W2995458275 hasRelatedWork W2090536060 @default.
- W2995458275 hasRelatedWork W2108627571 @default.
- W2995458275 hasRelatedWork W2127016580 @default.
- W2995458275 hasRelatedWork W2134559572 @default.
- W2995458275 hasRelatedWork W2142848335 @default.
- W2995458275 hasRelatedWork W2167982422 @default.
- W2995458275 hasRelatedWork W2400548961 @default.
- W2995458275 isParatext "false" @default.
- W2995458275 isRetracted "false" @default.
- W2995458275 magId "2995458275" @default.
- W2995458275 workType "dissertation" @default.