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- W2995472912 endingPage "102772" @default.
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- W2995472912 abstract "Topoisomerase poisons are known to stabilize covalent enzyme-DNA intermediates forming covalent cleavage complexes, which are highly cytotoxic especially for dividing cells and thus, make topoisomerases targets for cancer therapy. Topoisomerases have been extensively studied in mammalian model systems, whereas in other vertebrate models including zebrafish, they still remain less characterized. Here we show similarities in the genotoxic effects of zebrafish and mammalian systems towards topoisomerase I (Top1) poisons and PARP inhibitor – olaparib. On the other hand we observed that topoisomerase II (Top2) poisons (doxorubicin and etoposide) did not affect 1 day post fertilization embryo viability, however in cells isolated from Top2 drug treated embryos the formation of DNA cleavage complexes was observed by comet assay. We explain this by cellular drug uptake limitation in live zebrafish embryos versus unimpeded drug influx in cells isolated from Top2 poisons pre-treated embryos. We also demonstrate that EDTA facilitates the extraction of Top2 from zebrafish nuclei and recovers both, basal and Top2 poison induced DNA damage." @default.
- W2995472912 created "2019-12-26" @default.
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- W2995472912 date "2020-03-01" @default.
- W2995472912 modified "2023-09-23" @default.
- W2995472912 title "Genotoxic effects of topoisomerase poisoning and PARP inhibition on zebrafish embryos" @default.
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- W2995472912 doi "https://doi.org/10.1016/j.dnarep.2019.102772" @default.
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