Matches in SemOpenAlex for { <https://semopenalex.org/work/W2995488398> ?p ?o ?g. }
- W2995488398 endingPage "1183" @default.
- W2995488398 startingPage "1177" @default.
- W2995488398 abstract "The heterologous biosynthesis of complex natural products has enabled access to polyketide, nonribosomal peptide, isoprenoid, and other compounds with wide-spanning societal value. Though several surrogate host systems exist, Escherichia coli is often a preferred choice due to its rapid growth kinetics and extensive molecular biology protocols. However, a persistent challenge to the utilization of E. coli has been the successful in vivo reconstitution of type II polyketide synthase (PKS) systems. In particular, gene expression of the ketosynthase (KS) components of the minimal PKS has consistently yielded insoluble protein products. In the following report, two type II PKS systems were functionally reconstituted in E. coli. The approach to do so relied upon the utilization of the native transcriptional coupling between the dimeric KS subunits, leading to soluble recombinant protein products and successful polyketide biosynthesis. Resulting strains produced 10 mg/L TW95c and 25 mg/L dehydrorabelomycin. Hence, the strategy offers a new option in the biosynthetic engineering efforts for the heterologous production of type II polyketide products using E. coli." @default.
- W2995488398 created "2019-12-26" @default.
- W2995488398 creator A5014929343 @default.
- W2995488398 creator A5015625878 @default.
- W2995488398 creator A5018863416 @default.
- W2995488398 creator A5026396535 @default.
- W2995488398 creator A5043446520 @default.
- W2995488398 creator A5044227760 @default.
- W2995488398 creator A5056384718 @default.
- W2995488398 creator A5079480513 @default.
- W2995488398 creator A5089035316 @default.
- W2995488398 date "2019-12-11" @default.
- W2995488398 modified "2023-10-16" @default.
- W2995488398 title "Heterologous Biosynthesis of Type II Polyketide Products Using <i>E. coli</i>" @default.
- W2995488398 cites W1970247177 @default.
- W2995488398 cites W1988643982 @default.
- W2995488398 cites W1995071127 @default.
- W2995488398 cites W2003000734 @default.
- W2995488398 cites W2003706451 @default.
- W2995488398 cites W2017271290 @default.
- W2995488398 cites W2035378670 @default.
- W2995488398 cites W2036080658 @default.
- W2995488398 cites W2043165163 @default.
- W2995488398 cites W2053878100 @default.
- W2995488398 cites W2055509346 @default.
- W2995488398 cites W2056877619 @default.
- W2995488398 cites W2057256243 @default.
- W2995488398 cites W2063212143 @default.
- W2995488398 cites W2071446702 @default.
- W2995488398 cites W2073327466 @default.
- W2995488398 cites W2078610664 @default.
- W2995488398 cites W2101989580 @default.
- W2995488398 cites W2122091356 @default.
- W2995488398 cites W2159489517 @default.
- W2995488398 cites W2194023039 @default.
- W2995488398 cites W2214273798 @default.
- W2995488398 cites W2224175912 @default.
- W2995488398 cites W2256742438 @default.
- W2995488398 cites W2467031173 @default.
- W2995488398 cites W2476925648 @default.
- W2995488398 cites W2547783069 @default.
- W2995488398 cites W2745396386 @default.
- W2995488398 cites W2759658772 @default.
- W2995488398 cites W2783434395 @default.
- W2995488398 cites W2800771128 @default.
- W2995488398 cites W2904790785 @default.
- W2995488398 cites W2909909436 @default.
- W2995488398 cites W2955715715 @default.
- W2995488398 cites W2970940594 @default.
- W2995488398 cites W64124356 @default.
- W2995488398 doi "https://doi.org/10.1021/acschembio.9b00827" @default.
- W2995488398 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31825590" @default.
- W2995488398 hasPublicationYear "2019" @default.
- W2995488398 type Work @default.
- W2995488398 sameAs 2995488398 @default.
- W2995488398 citedByCount "27" @default.
- W2995488398 countsByYear W29954883982020 @default.
- W2995488398 countsByYear W29954883982021 @default.
- W2995488398 countsByYear W29954883982022 @default.
- W2995488398 countsByYear W29954883982023 @default.
- W2995488398 crossrefType "journal-article" @default.
- W2995488398 hasAuthorship W2995488398A5014929343 @default.
- W2995488398 hasAuthorship W2995488398A5015625878 @default.
- W2995488398 hasAuthorship W2995488398A5018863416 @default.
- W2995488398 hasAuthorship W2995488398A5026396535 @default.
- W2995488398 hasAuthorship W2995488398A5043446520 @default.
- W2995488398 hasAuthorship W2995488398A5044227760 @default.
- W2995488398 hasAuthorship W2995488398A5056384718 @default.
- W2995488398 hasAuthorship W2995488398A5079480513 @default.
- W2995488398 hasAuthorship W2995488398A5089035316 @default.
- W2995488398 hasConcept C104317684 @default.
- W2995488398 hasConcept C185592680 @default.
- W2995488398 hasConcept C2778635478 @default.
- W2995488398 hasConcept C2780115692 @default.
- W2995488398 hasConcept C547475151 @default.
- W2995488398 hasConcept C553450214 @default.
- W2995488398 hasConcept C55493867 @default.
- W2995488398 hasConcept C71240020 @default.
- W2995488398 hasConcept C86803240 @default.
- W2995488398 hasConceptScore W2995488398C104317684 @default.
- W2995488398 hasConceptScore W2995488398C185592680 @default.
- W2995488398 hasConceptScore W2995488398C2778635478 @default.
- W2995488398 hasConceptScore W2995488398C2780115692 @default.
- W2995488398 hasConceptScore W2995488398C547475151 @default.
- W2995488398 hasConceptScore W2995488398C553450214 @default.
- W2995488398 hasConceptScore W2995488398C55493867 @default.
- W2995488398 hasConceptScore W2995488398C71240020 @default.
- W2995488398 hasConceptScore W2995488398C86803240 @default.
- W2995488398 hasFunder F4320321001 @default.
- W2995488398 hasFunder F4320321540 @default.
- W2995488398 hasFunder F4320327100 @default.
- W2995488398 hasIssue "5" @default.
- W2995488398 hasLocation W29954883981 @default.
- W2995488398 hasOpenAccess W2995488398 @default.
- W2995488398 hasPrimaryLocation W29954883981 @default.
- W2995488398 hasRelatedWork W1494491945 @default.
- W2995488398 hasRelatedWork W1978047260 @default.
- W2995488398 hasRelatedWork W1994361647 @default.