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- W2995514573 abstract "Primordial germ cells (PGCs) are precursors of eggs and sperm. How the PGCs epigenetically reprogram during early embryonic development in fish is currently unknown. Here we generated a series of PGC methylomes using whole genome bisulfite sequencing across key stages from 8 days post fertilization (dpf) to 25 dpf coinciding with germ cell sex determination and gonadal sex differentiation in medaka (Oryzias latipes) to elucidate the dynamics of DNA methylation during epigenetic reprogramming in germ cells. Our high-resolution DNA methylome maps show a global demethylation taking place in medaka PGCs in a two-step strategy. The first step occurs between the blastula and 8-dpf stages, and the second step occurs between the 10-dpf and 12-dpf stages. Both demethylation processes are global, except for CGI promoters which remain hypomethylated throughout the stage of PGC specification. De novo methylation proceeded at 25-dpf stage with the process in male germ cells superseding female germ cells. Gene expression analysis showed that tet2 maintains high levels of expression during the demethylation stage, while dnmt3ba expression increases during the de novo methylation stage during sexual fate determination in germ cells. The present results suggest that medaka PGCs undergo a bi-phasic epigenetic reprogramming process. Global erasure of DNA methylation marks peaks at 15-dpf and de novo methylation in male germ cells takes precedence over female germ cells at 25 dpf. Results also provide important insights into the developmental window of susceptibility to environmental stressors for multi- and trans-generational health outcomes in fish." @default.
- W2995514573 created "2019-12-26" @default.
- W2995514573 creator A5036929190 @default.
- W2995514573 creator A5067315031 @default.
- W2995514573 date "2019-12-18" @default.
- W2995514573 modified "2023-09-23" @default.
- W2995514573 title "The dynamics of DNA methylation during epigenetic reprogramming of primordial germ cells in medaka (<i>Oryzias latipes</i>)" @default.
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- W2995514573 doi "https://doi.org/10.1080/15592294.2019.1695341" @default.
- W2995514573 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7188396" @default.
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- W2995514573 hasPublicationYear "2019" @default.
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