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• W2995536064 abstract "Abstract Background We report an improved assembly and scaffolding of the European pear (Pyrus communis L.) genome (referred to as BartlettDHv2.0), obtained using a combination of Pacific Biosciences RSII long-read sequencing, Bionano optical mapping, chromatin interaction capture (Hi-C), and genetic mapping. The sample selected for sequencing is a double haploid derived from the same “Bartlett” reference pear that was previously sequenced. Sequencing of di-haploid plants makes assembly more tractable in highly heterozygous species such as P. communis. Findings A total of 496.9 Mb corresponding to 97% of the estimated genome size were assembled into 494 scaffolds. Hi-C data and a high-density genetic map allowed us to anchor and orient 87% of the sequence on the 17 pear chromosomes. Approximately 50% (247 Mb) of the genome consists of repetitive sequences. Gene annotation confirmed the presence of 37,445 protein-coding genes, which is 13% fewer than previously predicted. Conclusions We showed that the use of a doubled-haploid plant is an effective solution to the problems presented by high levels of heterozygosity and duplication for the generation of high-quality genome assemblies. We present a high-quality chromosome-scale assembly of the European pear Pyrus communis and demostrate its high degree of synteny with the genomes of Malus x Domestica and Pyrus x bretschneideri." @default.
• W2995536064 created "2019-12-26" @default.
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• W2995536064 date "2019-12-01" @default.
• W2995536064 modified "2023-10-18" @default.
• W2995536064 title "Pseudo-chromosome–length genome assembly of a double haploid “Bartlett” pear (Pyrus communis L.)" @default.
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• W2995536064 doi "https://doi.org/10.1093/gigascience/giz138" @default.
• W2995536064 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6901071" @default.
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