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• W2995989117 endingPage "127560" @default.
• W2995989117 startingPage "127560" @default.
• W2995989117 abstract "The aberrant expression levels of reactive oxygen and nitrogen species (ROS and RNS) in human cells are related with many diseases, including cancer. In recent years, the primary RNS, peroxynitrite anion (ONOO−), has aroused immense research interest in the biological and biomedical fields. Herein, we build a novel sensing platform by the hollow tubular organic conjugated microporous polymer (HTCMPs) modified glassy carbon electrode (GCE) for directly monitoring of ONOO−. Specifically, the HTCMP was used to develop an electrochemical sensor for the first time. This as-prepared amperometric sensor showed an excellent activity toward the oxidation of ONOO− with a wide range of four orders of magnitude and a super low detection limit reached 1.54 ± 0.5 nM (S/N = 3). With these properties, the sensor was used for measuring the ONOO− released from glioma cells (U87) during oxidative stress induced by biochemical reagent stimulation. For the more, a new strategy to verify antioxidant protection was developed by stimulating cells at low or high doses of stimulus in a vitro simulation environment. The results showed that reduced glutathione (GSH) had great protective effect on cells in the case of oxidative stress. Last but not least, based on a battery of control tests, the concentrations of two key ROS/RNS (H2O2, ONOO−) in the cells were determined. This study essentially offers a reproducible platform for assessing the oxidative stress metabolites (ROS/RNS) in a vivo assays." @default.
• W2995989117 created "2019-12-26" @default.
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• W2995989117 date "2020-03-01" @default.
• W2995989117 modified "2023-09-30" @default.
• W2995989117 title "Direct sensing of peroxynitrite anion at sensitive hollow tubular organic conjugated microporous polymers modified electrode. Application to selective analysis of ROS and RNS in cells" @default.
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• W2995989117 doi "https://doi.org/10.1016/j.snb.2019.127560" @default.
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