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• W2996388632 abstract "OZET Amac: Hucre ici kalsiyum (Ca2+) sinyali; hucre proliferasyonu ve farklilasmasi, gen transkripsiyonu, apoptozis gibi bircok hucresel surecte rol oynar. Yapilan calismalarda Ca2+ sinyalinin kanser ile iliskili oldugu gosterilmistir. Transient receptor potential vanilloid 4 (TRPV4) kanallari hucre zarinda bulunan Ca2+ gecirgen non-selekif katyon kanalidir. TRPV4 kanallari vucutta bircok dokuda yaygin olarak eksprese edilmektedir. Hucre ici Ca2+ miktarinin duzenlenmesinde etkisi olan TRPV4 kanallarinin kanser hucrelerinin proliferasyonu, apoptozis, migrasyonu ve tumor anjiogenezi ile ilgili onemli rolleri oldugu gorulmustur. Biz bu calismada TRPV4 antagonisti olan RN 1734’un, insan prostat (PC-3) ve insan meme (MCF-7) kanseri hucre hatlarinda hucre canliligi ve DNA hasari uzerine etkilerini arastirdik. Gerec ve Yontemler: Calismamizda insan prostat (PC-3) ve insan meme (MCF-7) kanseri hucre hatlari kullanildi. RN 1734 1, 5, 25, 50 ve 100 µM’lik konsantrasyonlarda, 24 saatlik PC-3 ve MCF-7 hucre canliligina olan etkisi 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromid (MTT) yontemiyle olculdu. RN 1734’un DNA hasarina etkisi Comet yontemine gore belirlendi. Bulgular: 24 saat sureyle RN 1734’un ozellikle 50 ve 100 µM’lik konsantrasyonlariyla inkube edilen PC-3 ve MCF-7 hucrelerde canlilik duzeyi onemli olcude azaldi (p<0.05). Ayrica 100 µM’lik konsantrasyonda RN 1734 uygulamasi sonrasi hucre DNA hasari duzeyinde anlamli artisin oldugu goruldu (p<0.05). Sonuc: Biz bu calismada TRPV4 antagonisti olan RN 1734'un, PC-3 ve MCF-7 hucre hatlarinda hucre canliligini azalttigini ve DNA hasarina neden oldugunu bulduk. Bizim bulgularimiz, TRPV4 antagonisti uygulanmasinin kanser hucrelerine karsi yeni bir tedavi yaklasimi olabilecegini dusundurmektedir. Anahtar kelimeler: RN 1734; TRPV4; Kanser; PC-3; MCF-7; Comet yontemi; MTT ABSTRACT Aim: Intracellular calcium (Ca2+) signal pathway has a role in cell proliferation and differentiation, gene transcription and apoptosis. Studies have shown that Ca2+ signal is related to cancer. Transient receptor potential vanilloid 4 channels are Ca2+ permeable non-selective cation channels in the cell membrane. TRPV4 channels are commonly expressed in many tissues in the body. TRPV4 channels, which have an effect on the regulation of intracellular Ca2+ levels, have been shown to have important roles in the proliferation, apoptosis, migration and tumor angiogenesis of cancer cells. In this study, we investigated the effects of RN 1734, TRPV4 antagonist, on cell viability and DNA damage in human prostate (PC-3) and human breast (MCF-7) cancer cell lines. Materials and Methods: Human prostate (PC-3) and human breast (MCF-7) cancer cell lines were used in our study. The effect of RN 1734 on 1, 5, 25, 50 and 100 µM concentrations on 24-hour PC-3 and MCF-7 cell viability was determined by 3- (4,5-dimethylthiazol-2-yl) -2,5- diphenyltetrazolium bromide (MTT) method. The effect of RN 1734 on DNA damage was determined according to Comet Assay method. Results: The viability of PC-3 and MCF-7 cells, which were incubated for 24 hours with RN 1734, especially at concentrations of 50 and 100 µM, was significantly reduced (p<0.05). In addition, there was a significant increase in cell DNA damage level after RN 1734 100 µM concentration application (p <0.05). Conclusions: In this study, we found that RN 1734, TRPV4 antagonist, reduces cell viability and causes DNA damage in PC-3 and MCF-7 cell lines. Our findings suggest that administration of TRPV4 antagonist may be a new therapeutic approach to cancer cells. Keywords: RN 1734; TRPV4; Cancer; PC-3; MCF-7; Comet assay; MTT" @default.
• W2996388632 created "2019-12-26" @default.
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• W2996388632 date "2019-09-16" @default.
• W2996388632 modified "2023-09-27" @default.
• W2996388632 title "MCF-7 VE PC-3 HÜCRE HATLARINDA, HÜCRE CANLILIĞI VE DNA HASARI ÜZERİNE TRPV4 ANTAGONİSTİ RN 1734'ÜN ETKİLERİ Effect of TRPV4 Antagonist RN 1734 on DNA Damage and Cell Viability in MCF-7 and PC-3 Cell Lines" @default.
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