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• W2996571879 abstract "Cryogenic soft X-ray tomography (Cryo-SXT) is ideally suitable to image the 3D sub-cellular architecture and organization of cells with high resolution in the near-native preservation state. Cryogenic fluorescence microscopy (Cryo-FM) can determine the location of a molecule of interest that has been labeled with a fluorescent tag, thus revealing the function of the cells. To understand the relations between the sub-cellular architecture and the function of cells, correlative Cryo-SXT and Cryo-FM was applied. This method required the matching of images of different modalities, and the accuracy of the matching is important. Here, a precise correlative method of Cryo-SXT and Cryo-FM is introduced. The capability of matching images of different modalities with high resolution was verified by simulations and practical experiments, and the method was used to identify vacuoles and mitochondria." @default.
• W2996571879 created "2019-12-26" @default.
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• W2996571879 date "2020-01-01" @default.
• W2996571879 modified "2023-09-24" @default.
• W2996571879 title "Precise correlative method of Cryo-SXT and Cryo-FM for organelle identification" @default.
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• W2996571879 doi "https://doi.org/10.1107/s1600577519015194" @default.
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