Matches in SemOpenAlex for { <https://semopenalex.org/work/W2996635610> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2996635610 abstract "Abstract Background Precise targeting of patient’s tumor can be facilitated through T cells recognizing specific pMHC complexes on these tumors. Once such antigen landscape is defined, an additional challenge relates to the ability to mount a functional T cell response specific to such antigens. Current T Cell expansion techniques provide no efficient strategy for antigen-specific stimulation and results largely in exhausted T cells. Here we present a new technology for ex-vivo peptide-MHC directed expansion and functional enhancement of T cells, using artificial antigen-presenting scaffolds (Ag-scaffolds). Methods Dextran backbone conjugated with streptavidin was used for Ag-scaffold assembly. Such backbone was incubated together with biotinylated MHC class I molecules holding a given peptide (pMHC) and biotinylated human cytokines in various ratios. PBMCs from healthy donors with known virus epitopes and from melanoma patients with known epitopes were expanded for 2 weeks with Ag-scaffold, added when the cell cultures were initiated and twice per week for two weeks. The expansion of antigen-specific CD8 T cells was monitored and analyzed by flow cytometry. Results Here we demonstrate the value of the antigen-scaffold based expansion, by the expansion of virus-CD8 T cells from peripheral blood, and tumor-specific T cells from both peripheral blood and tumor site. We can favorably expand both neo- and shared antigen specific T cell populations. The resulting T cell product exhibit a multifunctional cytokine profile upon antigen challenge, high levels of CD28 expression, and reduced levels of PD-1 expression. Importantly, numerous different pMHC-specific T-cell populations can be stimulated in a single culture, and we can obtain better tumor killing properties than conventional TIL-ACT products. Conclusion This study presents a new technology for expanding functionally enhanced antigen-specific CD8 T cells suited for implementation to ACT strategies. T cell phenotype and functionality are known to be important parameters in successful adoptive T cell transfer, and hence it´s plausible that antigen scaffold based expanded T cell product may improve treatment outcome compared to conventional expansion strategies. Legal entity responsible for the study The authors. Funding Innovation Fund Denmark. Disclosure All authors have declared no conflicts of interest." @default.
- W2996635610 created "2019-12-26" @default.
- W2996635610 creator A5004823373 @default.
- W2996635610 creator A5025825017 @default.
- W2996635610 creator A5033417797 @default.
- W2996635610 creator A5033440492 @default.
- W2996635610 creator A5037539046 @default.
- W2996635610 creator A5040970798 @default.
- W2996635610 creator A5042840044 @default.
- W2996635610 creator A5053692443 @default.
- W2996635610 creator A5073488854 @default.
- W2996635610 creator A5073926805 @default.
- W2996635610 creator A5083713436 @default.
- W2996635610 creator A5052545546 @default.
- W2996635610 date "2019-12-01" @default.
- W2996635610 modified "2023-09-27" @default.
- W2996635610 title "TCR engaging antigen-scaffolds for targeted expansion of functionally improved T cells for adoptive cell therapy" @default.
- W2996635610 doi "https://doi.org/10.1093/annonc/mdz448.004" @default.
- W2996635610 hasPublicationYear "2019" @default.
- W2996635610 type Work @default.
- W2996635610 sameAs 2996635610 @default.
- W2996635610 citedByCount "0" @default.
- W2996635610 crossrefType "journal-article" @default.
- W2996635610 hasAuthorship W2996635610A5004823373 @default.
- W2996635610 hasAuthorship W2996635610A5025825017 @default.
- W2996635610 hasAuthorship W2996635610A5033417797 @default.
- W2996635610 hasAuthorship W2996635610A5033440492 @default.
- W2996635610 hasAuthorship W2996635610A5037539046 @default.
- W2996635610 hasAuthorship W2996635610A5040970798 @default.
- W2996635610 hasAuthorship W2996635610A5042840044 @default.
- W2996635610 hasAuthorship W2996635610A5052545546 @default.
- W2996635610 hasAuthorship W2996635610A5053692443 @default.
- W2996635610 hasAuthorship W2996635610A5073488854 @default.
- W2996635610 hasAuthorship W2996635610A5073926805 @default.
- W2996635610 hasAuthorship W2996635610A5083713436 @default.
- W2996635610 hasBestOaLocation W29966356101 @default.
- W2996635610 hasConcept C147483822 @default.
- W2996635610 hasConcept C148125776 @default.
- W2996635610 hasConcept C153911025 @default.
- W2996635610 hasConcept C154317977 @default.
- W2996635610 hasConcept C167672396 @default.
- W2996635610 hasConcept C195616568 @default.
- W2996635610 hasConcept C202751555 @default.
- W2996635610 hasConcept C203014093 @default.
- W2996635610 hasConcept C204628709 @default.
- W2996635610 hasConcept C2776090121 @default.
- W2996635610 hasConcept C55493867 @default.
- W2996635610 hasConcept C67662055 @default.
- W2996635610 hasConcept C71924100 @default.
- W2996635610 hasConcept C86803240 @default.
- W2996635610 hasConcept C8840205 @default.
- W2996635610 hasConcept C8891405 @default.
- W2996635610 hasConcept C95444343 @default.
- W2996635610 hasConceptScore W2996635610C147483822 @default.
- W2996635610 hasConceptScore W2996635610C148125776 @default.
- W2996635610 hasConceptScore W2996635610C153911025 @default.
- W2996635610 hasConceptScore W2996635610C154317977 @default.
- W2996635610 hasConceptScore W2996635610C167672396 @default.
- W2996635610 hasConceptScore W2996635610C195616568 @default.
- W2996635610 hasConceptScore W2996635610C202751555 @default.
- W2996635610 hasConceptScore W2996635610C203014093 @default.
- W2996635610 hasConceptScore W2996635610C204628709 @default.
- W2996635610 hasConceptScore W2996635610C2776090121 @default.
- W2996635610 hasConceptScore W2996635610C55493867 @default.
- W2996635610 hasConceptScore W2996635610C67662055 @default.
- W2996635610 hasConceptScore W2996635610C71924100 @default.
- W2996635610 hasConceptScore W2996635610C86803240 @default.
- W2996635610 hasConceptScore W2996635610C8840205 @default.
- W2996635610 hasConceptScore W2996635610C8891405 @default.
- W2996635610 hasConceptScore W2996635610C95444343 @default.
- W2996635610 hasLocation W29966356101 @default.
- W2996635610 hasOpenAccess W2996635610 @default.
- W2996635610 hasPrimaryLocation W29966356101 @default.
- W2996635610 hasRelatedWork W11113079 @default.
- W2996635610 hasRelatedWork W13366478 @default.
- W2996635610 hasRelatedWork W15718139 @default.
- W2996635610 hasRelatedWork W16608652 @default.
- W2996635610 hasRelatedWork W18724991 @default.
- W2996635610 hasRelatedWork W20133129 @default.
- W2996635610 hasRelatedWork W20203410 @default.
- W2996635610 hasRelatedWork W20285363 @default.
- W2996635610 hasRelatedWork W4628118 @default.
- W2996635610 hasRelatedWork W5305617 @default.
- W2996635610 isParatext "false" @default.
- W2996635610 isRetracted "false" @default.
- W2996635610 magId "2996635610" @default.
- W2996635610 workType "article" @default.