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• W2996708013 abstract "During molecular cloning, screening bacterial transformants is a time-consuming and labor-intensive process; however, tractable tools that can be applied to various vectors for visual confirmation of desired colonies are limited. Recently, we reported that translational enhancement by a Dictyostelium gene sequence (TED) boosted protein expression even without an expression inducer in Escherichia coli. Here, we demonstrate a generally applicable molecular tool using the expression of green fluorescent protein enhanced by TED. By inserting a module related to TED into the cloning site in advance, we effectively screened E. coli colonies harboring the desired plasmid functions in a prokaryote ( Magnetospirillum gryphiswaldense) or eukaryote ( Dictyostelium discoideum). Thus, our system represents a user-friendly technique for cloning." @default.
• W2996708013 created "2019-12-26" @default.
• W2996708013 creator A5016389989 @default.
• W2996708013 creator A5048870494 @default.
• W2996708013 date "2020-02-01" @default.
• W2996708013 modified "2023-10-14" @default.
• W2996708013 title "An improved molecular tool for screening bacterial colonies using GFP expression enhanced by a <i>Dictyostelium</i> sequence" @default.
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• W2996708013 doi "https://doi.org/10.2144/btn-2019-0127" @default.
• W2996708013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31825246" @default.
• W2996708013 hasPublicationYear "2020" @default.
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