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- W2996755561 abstract "Abstract Genotoxicity testing is critical for predicting adverse effects of pharmaceutical, industrial, and environmental chemicals. The alkaline comet assay is an established method for detecting DNA strand breaks, however, the assay does not detect potentially carcinogenic bulky adducts that can arise when metabolic enzymes convert pro-carcinogens into a highly DNA reactive products. To overcome this, we use DNA synthesis inhibitors (hydroxyurea and 1-β-d-arabinofuranosyl cytosine) to trap single strand breaks that are formed during nucleotide excision repair, which primarily removes bulky lesions. In this way, comet-undetectable bulky lesions are converted into comet-detectable single strand breaks. Moreover, we use HepaRG™ cells to recapitulate in vivo metabolic capacity, and leverage the CometChip platform (a higher throughput more sensitive comet assay) to create the ‘HepaCometChip’, enabling the detection of bulky genotoxic lesions that are missed by current genotoxicity screens. The HepaCometChip thus provides a broadly effective approach for detection of bulky DNA adducts." @default.
- W2996755561 created "2019-12-26" @default.
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- W2996755561 date "2019-12-11" @default.
- W2996755561 modified "2023-10-17" @default.
- W2996755561 title "Sensitive CometChip assay for screening potentially carcinogenic DNA adducts by trapping DNA repair intermediates" @default.
- W2996755561 cites W1487243507 @default.
- W2996755561 cites W1500217783 @default.
- W2996755561 cites W1514335015 @default.
- W2996755561 cites W1545611675 @default.
- W2996755561 cites W1571583248 @default.
- W2996755561 cites W1604405497 @default.
- W2996755561 cites W1607158544 @default.
- W2996755561 cites W1667328849 @default.
- W2996755561 cites W1746765074 @default.
- W2996755561 cites W1780228573 @default.
- W2996755561 cites W1821122251 @default.
- W2996755561 cites W1839220209 @default.
- W2996755561 cites W1870158705 @default.
- W2996755561 cites W1886853229 @default.
- W2996755561 cites W1904241335 @default.
- W2996755561 cites W1963838195 @default.
- W2996755561 cites W1965819376 @default.
- W2996755561 cites W1968499584 @default.
- W2996755561 cites W1972081789 @default.
- W2996755561 cites W1972094156 @default.
- W2996755561 cites W1974691766 @default.
- W2996755561 cites W1974993420 @default.
- W2996755561 cites W1979123830 @default.
- W2996755561 cites W1980455299 @default.
- W2996755561 cites W1980657599 @default.
- W2996755561 cites W1987194415 @default.
- W2996755561 cites W1989208996 @default.
- W2996755561 cites W1991127974 @default.
- W2996755561 cites W1992093074 @default.
- W2996755561 cites W1996613294 @default.
- W2996755561 cites W2003606716 @default.
- W2996755561 cites W2004125597 @default.
- W2996755561 cites W2004326614 @default.
- W2996755561 cites W2005239815 @default.
- W2996755561 cites W2006850981 @default.
- W2996755561 cites W2007771316 @default.
- W2996755561 cites W2009649574 @default.
- W2996755561 cites W2011079789 @default.
- W2996755561 cites W2013992515 @default.
- W2996755561 cites W2015221530 @default.
- W2996755561 cites W2018801161 @default.
- W2996755561 cites W2020391615 @default.
- W2996755561 cites W2021372862 @default.
- W2996755561 cites W2027254129 @default.
- W2996755561 cites W2027905068 @default.
- W2996755561 cites W2028603080 @default.
- W2996755561 cites W2029938020 @default.
- W2996755561 cites W2030459989 @default.
- W2996755561 cites W2030873172 @default.
- W2996755561 cites W2031816170 @default.
- W2996755561 cites W2034485370 @default.
- W2996755561 cites W2034562422 @default.
- W2996755561 cites W2038329078 @default.
- W2996755561 cites W2040150820 @default.
- W2996755561 cites W2042699531 @default.
- W2996755561 cites W2043610941 @default.
- W2996755561 cites W2048458737 @default.
- W2996755561 cites W2050028436 @default.
- W2996755561 cites W2059151331 @default.
- W2996755561 cites W2059340538 @default.
- W2996755561 cites W2060071482 @default.
- W2996755561 cites W2060371289 @default.
- W2996755561 cites W2061508611 @default.
- W2996755561 cites W2068076189 @default.
- W2996755561 cites W2071417758 @default.
- W2996755561 cites W2072145718 @default.
- W2996755561 cites W2072415705 @default.
- W2996755561 cites W2076110072 @default.
- W2996755561 cites W2078540764 @default.
- W2996755561 cites W2084369408 @default.
- W2996755561 cites W2086210951 @default.
- W2996755561 cites W2088909697 @default.
- W2996755561 cites W2090161761 @default.
- W2996755561 cites W2090445795 @default.
- W2996755561 cites W2092089811 @default.
- W2996755561 cites W2094219598 @default.
- W2996755561 cites W2094764447 @default.
- W2996755561 cites W2094932400 @default.
- W2996755561 cites W2095112842 @default.
- W2996755561 cites W2095901035 @default.
- W2996755561 cites W2096136751 @default.
- W2996755561 cites W2097124161 @default.