FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2996894063> ?p ?o ?g. }

• W2996894063 endingPage "235" @default.
• W2996894063 startingPage "224" @default.
• W2996894063 abstract "Immunotoxicity analysis receives a strong interest in environmental a priori and a posteriori risk assessment procedures considering the direct involvement of the immune system in the health status of organisms, populations and thus ecosystems. The freshwater mussel Dreissena polymorpha is an invasive species widely used in ecotoxicology studies and biomonitoring surveys to evaluate the impacts of contaminants on aquatic fauna. Bivalve hemocytes are the immunocompetent cells circulating in the open circulatory system of the organism. However, there is nowadays no consensus on a protocol to evaluate the immunocompetent state of this particular cell type using flow cytometry. Wild species such as D. polymorpha present several technical barriers complicating their analyze including (i) the quality and the purity of the hemolymph sample, (ii) the controversial characterization of hemocyte subpopulations and their diversity, (iii) the quantity of biological material, and (iv) the high inter-individual variability of hemocyte responses. The present work proposes several technical and analytical improvements to control the above-mentioned issues. The inclusion of sedimentation and cell detachment steps in the pre-analytical phase of the protocol substantially ameliorate the quality of the hemolymph sample as well as the accuracy of the cytometric measurements, by selecting the analyzed cells on their adhesion ability and by increasing the concentration of the analyzed events. The development of an effective triple-labeling procedure including the cellular probe Hoechst® 33342, the membrane impermeant dye propidium iodide and yellow-green fluorescent microspheres allowed the simultaneous analysis of cytotoxicity and phagocytosis activity in hemocytes. It also significantly enhanced the accuracy of hemocyte endpoint measurements by eliminating non-target events from the analysis and allowing relevant gating strategies. Finally, the use of pooled samples of hemolymph noticeably reduced inter-sample variability while providing more plasticity in the experimental design and improving the discriminating potency between treatments. The developed protocol is suitable for ex vivo exposure of hemocyte in a chemical/environmental toxicity assessment as well as for in vivo exposure in the laboratory or in situ biomonitoring surveys with few adaptations." @default.
• W2996894063 created "2020-01-10" @default.
• W2996894063 creator A5069355022 @default.
• W2996894063 creator A5081390012 @default.
• W2996894063 creator A5081620782 @default.
• W2996894063 creator A5090500826 @default.
• W2996894063 date "2020-03-01" @default.
• W2996894063 modified "2023-10-18" @default.
• W2996894063 title "A new protocol for the simultaneous flow cytometric analysis of cytotoxicity and immunotoxicity on zebra mussel (Dreissena polymorpha) hemocytes" @default.
• W2996894063 cites W1916871370 @default.
• W2996894063 cites W1968948521 @default.
• W2996894063 cites W1970346499 @default.
• W2996894063 cites W1978109821 @default.
• W2996894063 cites W1979235420 @default.
• W2996894063 cites W1989203097 @default.
• W2996894063 cites W1995027175 @default.
• W2996894063 cites W2011611780 @default.
• W2996894063 cites W2017961881 @default.
• W2996894063 cites W2027732306 @default.
• W2996894063 cites W2027979370 @default.
• W2996894063 cites W2028480207 @default.
• W2996894063 cites W2030063816 @default.
• W2996894063 cites W2044152887 @default.
• W2996894063 cites W2058232893 @default.
• W2996894063 cites W2067865038 @default.
• W2996894063 cites W2076494603 @default.
• W2996894063 cites W2080709353 @default.
• W2996894063 cites W2089599568 @default.
• W2996894063 cites W2094182473 @default.
• W2996894063 cites W2095173950 @default.
• W2996894063 cites W2114219560 @default.
• W2996894063 cites W2124195770 @default.
• W2996894063 cites W2156726495 @default.
• W2996894063 cites W2191818139 @default.
• W2996894063 cites W2289065530 @default.
• W2996894063 cites W2460096600 @default.
• W2996894063 cites W2561232481 @default.
• W2996894063 cites W2791445599 @default.
• W2996894063 cites W2795444750 @default.
• W2996894063 cites W2801641880 @default.
• W2996894063 cites W2938993835 @default.
• W2996894063 doi "https://doi.org/10.1016/j.fsi.2019.12.092" @default.
• W2996894063 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31899358" @default.
• W2996894063 hasPublicationYear "2020" @default.
• W2996894063 type Work @default.
• W2996894063 sameAs 2996894063 @default.
• W2996894063 citedByCount "12" @default.
• W2996894063 countsByYear W29968940632020 @default.
• W2996894063 countsByYear W29968940632021 @default.
• W2996894063 countsByYear W29968940632022 @default.
• W2996894063 countsByYear W29968940632023 @default.
• W2996894063 crossrefType "journal-article" @default.
• W2996894063 hasAuthorship W2996894063A5069355022 @default.
• W2996894063 hasAuthorship W2996894063A5081390012 @default.
• W2996894063 hasAuthorship W2996894063A5081620782 @default.
• W2996894063 hasAuthorship W2996894063A5090500826 @default.
• W2996894063 hasBestOaLocation W29968940631 @default.
• W2996894063 hasConcept C100068826 @default.
• W2996894063 hasConcept C109051061 @default.
• W2996894063 hasConcept C109316439 @default.
• W2996894063 hasConcept C18903297 @default.
• W2996894063 hasConcept C190283241 @default.
• W2996894063 hasConcept C202751555 @default.
• W2996894063 hasConcept C203014093 @default.
• W2996894063 hasConcept C2775934118 @default.
• W2996894063 hasConcept C2776249982 @default.
• W2996894063 hasConcept C2776846509 @default.
• W2996894063 hasConcept C2779492349 @default.
• W2996894063 hasConcept C2779586492 @default.
• W2996894063 hasConcept C2779987062 @default.
• W2996894063 hasConcept C31573885 @default.
• W2996894063 hasConcept C553184892 @default.
• W2996894063 hasConcept C55493867 @default.
• W2996894063 hasConcept C86803240 @default.
• W2996894063 hasConceptScore W2996894063C100068826 @default.
• W2996894063 hasConceptScore W2996894063C109051061 @default.
• W2996894063 hasConceptScore W2996894063C109316439 @default.
• W2996894063 hasConceptScore W2996894063C18903297 @default.
• W2996894063 hasConceptScore W2996894063C190283241 @default.
• W2996894063 hasConceptScore W2996894063C202751555 @default.
• W2996894063 hasConceptScore W2996894063C203014093 @default.
• W2996894063 hasConceptScore W2996894063C2775934118 @default.
• W2996894063 hasConceptScore W2996894063C2776249982 @default.
• W2996894063 hasConceptScore W2996894063C2776846509 @default.
• W2996894063 hasConceptScore W2996894063C2779492349 @default.
• W2996894063 hasConceptScore W2996894063C2779586492 @default.
• W2996894063 hasConceptScore W2996894063C2779987062 @default.
• W2996894063 hasConceptScore W2996894063C31573885 @default.
• W2996894063 hasConceptScore W2996894063C553184892 @default.
• W2996894063 hasConceptScore W2996894063C55493867 @default.
• W2996894063 hasConceptScore W2996894063C86803240 @default.
• W2996894063 hasFunder F4320335322 @default.
• W2996894063 hasLocation W29968940631 @default.
• W2996894063 hasLocation W29968940632 @default.
• W2996894063 hasLocation W29968940633 @default.
• W2996894063 hasLocation W29968940634 @default.
• W2996894063 hasLocation W29968940635 @default.
• W2996894063 hasLocation W29968940636 @default.

• next