FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2997084734> ?p ?o ?g. }

• W2997084734 endingPage "1215" @default.
• W2997084734 startingPage "1207" @default.
• W2997084734 abstract "Ankyrin-G (AnkG), a highly enriched scaffold protein in the axon initial segment (AIS) of neurons, functions to maintain axonal polarity and the integrity of the AIS. At the AIS, AnkG regulates selective intracellular cargo trafficking between soma and axons via interaction with the dynein regulator protein Ndel1, but the molecular mechanism underlying this binding remains elusive. Here we report that Ndel1’s C-terminal coiled-coil region (CT-CC) binds to giant neuron-specific insertion regions present in both AnkG and AnkB with 2:1 stoichiometry. The high-resolution crystal structure of AnkB in complex with Ndel1 CT-CC revealed the detailed molecular basis governing the AnkB/Ndel1 complex formation. Mechanistically, AnkB binds with Ndel1 by forming a stable 5-helix bundle dominated by hydrophobic interactions spread across 6 distinct interaction layers. Moreover, we found that AnkG is essential for Ndel1 accumulation at the AIS. Finally, we found that cargo sorting at the AIS can be disrupted by blocking the AnkG/Ndel1 complex formation using a peptide designed based on our structural data. Collectively, the atomic structure of the AnkB/Ndel1 complex together with studies of cargo sorting through the AIS establish the mechanistic basis for AnkG/Ndel1 complex formation and for the maintenance of axonal polarity. Our study will also be valuable for future studies of the interaction between AnkB and Ndel1 perhaps at distal axonal cargo transport." @default.
• W2997084734 created "2020-01-10" @default.
• W2997084734 creator A5021163747 @default.
• W2997084734 creator A5024385167 @default.
• W2997084734 creator A5075922236 @default.
• W2997084734 creator A5080407757 @default.
• W2997084734 creator A5084588183 @default.
• W2997084734 creator A5086664284 @default.
• W2997084734 date "2019-12-30" @default.
• W2997084734 modified "2023-10-09" @default.
• W2997084734 title "Mechanistic insights into the interactions of dynein regulator Ndel1 with neuronal ankyrins and implications in polarity maintenance" @default.
• W2997084734 cites W1652102492 @default.
• W2997084734 cites W1887408233 @default.
• W2997084734 cites W1963764308 @default.
• W2997084734 cites W1985485809 @default.
• W2997084734 cites W1989809732 @default.
• W2997084734 cites W1996616735 @default.
• W2997084734 cites W2004304118 @default.
• W2997084734 cites W2004368634 @default.
• W2997084734 cites W2012961395 @default.
• W2997084734 cites W2013655553 @default.
• W2997084734 cites W2026750744 @default.
• W2997084734 cites W2036623899 @default.
• W2997084734 cites W2037835429 @default.
• W2997084734 cites W2039469316 @default.
• W2997084734 cites W2039919932 @default.
• W2997084734 cites W2043174693 @default.
• W2997084734 cites W2044098590 @default.
• W2997084734 cites W2055764374 @default.
• W2997084734 cites W2066032422 @default.
• W2997084734 cites W2067890396 @default.
• W2997084734 cites W2085393352 @default.
• W2997084734 cites W2099647795 @default.
• W2997084734 cites W2103705255 @default.
• W2997084734 cites W2108590463 @default.
• W2997084734 cites W2108653140 @default.
• W2997084734 cites W2109004041 @default.
• W2997084734 cites W2120713647 @default.
• W2997084734 cites W2121978205 @default.
• W2997084734 cites W2123342169 @default.
• W2997084734 cites W2124276310 @default.
• W2997084734 cites W2124303196 @default.
• W2997084734 cites W2125760448 @default.
• W2997084734 cites W2131255875 @default.
• W2997084734 cites W2150038825 @default.
• W2997084734 cites W2161458853 @default.
• W2997084734 cites W2165073216 @default.
• W2997084734 cites W2198355387 @default.
• W2997084734 cites W2263074113 @default.
• W2997084734 cites W2415591668 @default.
• W2997084734 cites W2415595698 @default.
• W2997084734 cites W2508219669 @default.
• W2997084734 cites W2530097218 @default.
• W2997084734 cites W2558469082 @default.
• W2997084734 cites W2562253178 @default.
• W2997084734 cites W2747117709 @default.
• W2997084734 cites W2752756088 @default.
• W2997084734 cites W2758801732 @default.
• W2997084734 cites W2767175217 @default.
• W2997084734 cites W2798508824 @default.
• W2997084734 cites W2799700836 @default.
• W2997084734 cites W2804756885 @default.
• W2997084734 cites W2949873165 @default.
• W2997084734 cites W2961212325 @default.
• W2997084734 cites W2970469701 @default.
• W2997084734 doi "https://doi.org/10.1073/pnas.1916987117" @default.
• W2997084734 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6969496" @default.
• W2997084734 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31889000" @default.
• W2997084734 hasPublicationYear "2019" @default.
• W2997084734 type Work @default.
• W2997084734 sameAs 2997084734 @default.
• W2997084734 citedByCount "14" @default.
• W2997084734 countsByYear W29970847342020 @default.
• W2997084734 countsByYear W29970847342021 @default.
• W2997084734 countsByYear W29970847342022 @default.
• W2997084734 countsByYear W29970847342023 @default.
• W2997084734 crossrefType "journal-article" @default.
• W2997084734 hasAuthorship W2997084734A5021163747 @default.
• W2997084734 hasAuthorship W2997084734A5024385167 @default.
• W2997084734 hasAuthorship W2997084734A5075922236 @default.
• W2997084734 hasAuthorship W2997084734A5080407757 @default.
• W2997084734 hasAuthorship W2997084734A5084588183 @default.
• W2997084734 hasAuthorship W2997084734A5086664284 @default.
• W2997084734 hasBestOaLocation W29970847342 @default.
• W2997084734 hasConcept C104317684 @default.
• W2997084734 hasConcept C106987784 @default.
• W2997084734 hasConcept C113246987 @default.
• W2997084734 hasConcept C182800266 @default.
• W2997084734 hasConcept C202751555 @default.
• W2997084734 hasConcept C20418707 @default.
• W2997084734 hasConcept C53645450 @default.
• W2997084734 hasConcept C55493867 @default.
• W2997084734 hasConcept C62478195 @default.
• W2997084734 hasConcept C86803240 @default.
• W2997084734 hasConcept C95444343 @default.
• W2997084734 hasConceptScore W2997084734C104317684 @default.
• W2997084734 hasConceptScore W2997084734C106987784 @default.
• W2997084734 hasConceptScore W2997084734C113246987 @default.

• next