Matches in SemOpenAlex for { <https://semopenalex.org/work/W2997157247> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2997157247 endingPage "203" @default.
- W2997157247 startingPage "199" @default.
- W2997157247 abstract "Stevia rebaudiana plants produce non-caloric sweeteners that can be used as an alternative to sugar and also synthetic sweetening agents. Under natural conditions, seed germination is poor, and propagation through stem cuttings has limitations due to poor rooting. In the present study, a protocol was developed to obtain healthy plants of S. rebaudiana through organogenesis via callus cultures. Freshly collected seeds were surface sterilized using 0.2% Carbendazim with 5 minutes exposure time and 10% Clorox with 10 minutes exposure time. Seeds were then cultured on MS medium supplemented with 3.0 mgL-1 GA3 to obtain to In-Vitro germinated seedlings. Leaf discs (1cm2) and nodal explants (1 cm) were taken from 2 weeks old seedlings and cultured on MS medium supplemented with different concentrations and combinations of BAP and NAA. MS medium supplemented with 2.0 mgL-1 BAP and 1.0 mgL-1NAA was found to be the best medium for callus induction. To determine the best explant source, leaf discs, intermodal and nodal segments from seedlings were cultured on best callus induction medium, and leaf disc was found to be the best explants type for callus initiation. Leaf derived calli were transferred into shoot induction media and MS medium supplemented with 0.5 mgL-1 NAA and 2.0 mgL-1BAP was found to be the best medium for shoot induction. Shoots more than 5.0 cm in length were transferred into root induction media and half-strength MS medium supplemented with 0.5 mgL-1 IBA was found to be the best medium for root induction." @default.
- W2997157247 created "2020-01-10" @default.
- W2997157247 creator A5046710230 @default.
- W2997157247 creator A5048694816 @default.
- W2997157247 date "2019-07-01" @default.
- W2997157247 modified "2023-09-24" @default.
- W2997157247 title "In vitro plant regeneration of Stevia rebaudiana through indirect organogenesis" @default.
- W2997157247 hasPublicationYear "2019" @default.
- W2997157247 type Work @default.
- W2997157247 sameAs 2997157247 @default.
- W2997157247 citedByCount "0" @default.
- W2997157247 crossrefType "journal-article" @default.
- W2997157247 hasAuthorship W2997157247A5046710230 @default.
- W2997157247 hasAuthorship W2997157247A5048694816 @default.
- W2997157247 hasConcept C100701293 @default.
- W2997157247 hasConcept C104317684 @default.
- W2997157247 hasConcept C144027150 @default.
- W2997157247 hasConcept C157890192 @default.
- W2997157247 hasConcept C187528503 @default.
- W2997157247 hasConcept C200806820 @default.
- W2997157247 hasConcept C202751555 @default.
- W2997157247 hasConcept C21410773 @default.
- W2997157247 hasConcept C2780822416 @default.
- W2997157247 hasConcept C2992134379 @default.
- W2997157247 hasConcept C30503417 @default.
- W2997157247 hasConcept C55493867 @default.
- W2997157247 hasConcept C59822182 @default.
- W2997157247 hasConcept C86803240 @default.
- W2997157247 hasConcept C9549007 @default.
- W2997157247 hasConceptScore W2997157247C100701293 @default.
- W2997157247 hasConceptScore W2997157247C104317684 @default.
- W2997157247 hasConceptScore W2997157247C144027150 @default.
- W2997157247 hasConceptScore W2997157247C157890192 @default.
- W2997157247 hasConceptScore W2997157247C187528503 @default.
- W2997157247 hasConceptScore W2997157247C200806820 @default.
- W2997157247 hasConceptScore W2997157247C202751555 @default.
- W2997157247 hasConceptScore W2997157247C21410773 @default.
- W2997157247 hasConceptScore W2997157247C2780822416 @default.
- W2997157247 hasConceptScore W2997157247C2992134379 @default.
- W2997157247 hasConceptScore W2997157247C30503417 @default.
- W2997157247 hasConceptScore W2997157247C55493867 @default.
- W2997157247 hasConceptScore W2997157247C59822182 @default.
- W2997157247 hasConceptScore W2997157247C86803240 @default.
- W2997157247 hasConceptScore W2997157247C9549007 @default.
- W2997157247 hasIssue "4" @default.
- W2997157247 hasLocation W29971572471 @default.
- W2997157247 hasOpenAccess W2997157247 @default.
- W2997157247 hasPrimaryLocation W29971572471 @default.
- W2997157247 hasRelatedWork W1485760767 @default.
- W2997157247 hasRelatedWork W1578427814 @default.
- W2997157247 hasRelatedWork W2013361486 @default.
- W2997157247 hasRelatedWork W2111831733 @default.
- W2997157247 hasRelatedWork W2116916173 @default.
- W2997157247 hasRelatedWork W2182896241 @default.
- W2997157247 hasRelatedWork W2188853148 @default.
- W2997157247 hasRelatedWork W2241977010 @default.
- W2997157247 hasRelatedWork W2307516561 @default.
- W2997157247 hasRelatedWork W2620910096 @default.
- W2997157247 hasRelatedWork W2760373824 @default.
- W2997157247 hasRelatedWork W2893885369 @default.
- W2997157247 hasRelatedWork W2991637912 @default.
- W2997157247 hasRelatedWork W2991951427 @default.
- W2997157247 hasRelatedWork W3038423818 @default.
- W2997157247 hasRelatedWork W3198641874 @default.
- W2997157247 hasRelatedWork W54659226 @default.
- W2997157247 hasRelatedWork W2141010365 @default.
- W2997157247 hasRelatedWork W2182345374 @default.
- W2997157247 hasRelatedWork W2334437682 @default.
- W2997157247 hasVolume "4" @default.
- W2997157247 isParatext "false" @default.
- W2997157247 isRetracted "false" @default.
- W2997157247 magId "2997157247" @default.
- W2997157247 workType "article" @default.